Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,100,540	G→A	F256F (TTC→TTT)	SAUSA300_RS10590 ←	phage tail tape measure protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,100,540	0	G	A	100.0%	10.9 / NA	6	F256F (TTC→TTT)	SAUSA300_RS10590	phage tail tape measure protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (2/4);  total (2/4)

CCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTCAAGTTCTTTACCTGTAAAACCTAATCTTGTATTAACTTCTCCTAAAACTCCACCAACAGTTTCAGC  >  NC_007793/2100482‑2100635                                                           |                                                                                                ccATTGCACGCGTAATTAATTGAACGGCTTGTACGCCTTCAGAACCTGTTATGTGACTAAATTTCAAGAATGACTCTGTGGCACTCTCAAGTTCTTTGcc                                                        <  1:2939674/100‑1 (MQ=255)     tGCACGCGTAATTAATTGAACGGCTTGTACGCCTTCAGAACCTGTTATGTGACTAAATTTCAAGAATGACTCTGTGGCACTCTCAAGTTCTTTGCCAGtg                                                    >  1:5082703/1‑99 (MQ=255)                                   gccttCAGAACCTGTTATGTGACTAAATTTCAAGAATGACTCTGTGGCACTCTCAAGTTCTTTGCCAGTGAAACCTAACCTTGTGTTAACTTCCCCTaaa                      <  1:1465880/96‑1 (MQ=255)                                        cAGAACCTGTTATGTGACTAAATTTCAAGAATGACTCTGTGGCACTCTCAAGTTCTTTGCCAGTGAAACCTAACCTTGTGTTAACTTCCCCTAAAACAcc                 >  1:5734136/1‑100 (MQ=255)                                            aCCTGTTATGTGACTAAATTTCAAGAATGACTCTGTGGCACTCTCAAGTTCTTTGCCAGTGAAACCTAACCTTGTGTTAACTTCCCCTAAAACAccgcct             <  1:4886600/100‑2 (MQ=255)                                                       gACTAAATTTCAAGAATGACTCTGTGGCACTCTCAAGTTCTTTGCCAGTGAAACCTAACCTTGTGTTAACTTCCCCTAAAACACCGCCTACAGTCTCAgg  <  1:3756826/100‑2 (MQ=255)                                                           |                                                                                                CCATTGCACGGGTAATTAACTGTACGGCTTGCACACCGTCAGAACCTGTTATATGACTGAATTTCAAGAATGACTCTGTGGCATTTTCAAGTTCTTTACCTGTAAAACCTAATCTTGTATTAACTTCTCCTAAAACTCCACCAACAGTTTCAGC  >  NC_007793/2100482‑2100635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑