Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,100,276	T→G	A344A (GCA→GCC)	SAUSA300_RS10590 ←	phage tail tape measure protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,100,276	0	T	G	100.0%	13.6 / NA	7	A344A (GCA→GCC)	SAUSA300_RS10590	phage tail tape measure protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (2/5);  total (2/5)

CGTCTTTTCAATTTCTGCTAATGTCTTCTTAAATTCTTCTCTTGGGTTTTTACCAGCTTTACCCCAATTTGATATAGCTTTTTTCAAACCACTGAATGCTATTTCAGTATTAACGCCTGACTTTTCCCATTGAGAGAATAAAGCAATTGATTCTTTCATCTCAAAGCCCATAGCTCTCATTGGAGC  >  NC_007793/2100180‑2100365                                                                                                 |                                                                                          atctttttCAATTTCTGCTAATGTCTTCTTAAATTCTTCTCTTGGATTTTTACCAGCTTTACCCCAATTGGATATAGCTTTTTTCAAACCACTGAAGGcc                                                                                        <  1:5487678/96‑2 (MQ=255)                    aaTGTCTTCTTAAATTCTTCTCTTGGATTTTTACCAGCTTTACCCCAATTGGATATAGCTTTTTTCAAACCACTGAAGGCTATTTCAGTATTAACACCTg                                                                     <  1:3695985/100‑1 (MQ=255)                       gTCTTCTTAAATTCTTCTCTTGGATTTTTACCAGCTTTACCCCAATTGGATATAGCTTTTTTCAAACCACTGAAGGCTATTTCAGTATTAACACCTGatt                                                                  >  1:5253933/1‑98 (MQ=255)                                                ttttACCAGCTTTACCCCAATTGGATACAGCTTTTTTCAAACCACTGAAGGCTATTTCAGTATTAACACCTGATTTCTCCCATTGAGAGAATAAAGcgag                                         <  1:5146678/100‑4 (MQ=255)                                                            tACCCCAATTGGATATAGCTTTTTTCAAACCACTGAAGGCTATTTCAGTATTAACACCTGATTTCTCCCATTGAGAGAATAAAGCGATTGATTCTTTCAt                             >  1:2111466/1‑100 (MQ=255)                                                                                    tCAAACCACTGAAGGCTATTTCAGTATTAACACCTGATTTCTCCCATTGAGAGAATAAAGCGATTGATTCTTTCATCTCAAAGCCCATAGCCCTCATTgg     <  1:1484825/100‑1 (MQ=255)                                                                                       aaCCACTGAAGGCTATTTCAGTATTAACACCTGATTTCTCCCATTGAGAGAATAAAGCGATTGATTCTTTCATCTCAAAGCCCATAGCCCTCATTGGAgc  <  1:5570728/100‑1 (MQ=255)                                                                                                 |                                                                                          CGTCTTTTCAATTTCTGCTAATGTCTTCTTAAATTCTTCTCTTGGGTTTTTACCAGCTTTACCCCAATTTGATATAGCTTTTTTCAAACCACTGAATGCTATTTCAGTATTAACGCCTGACTTTTCCCATTGAGAGAATAAAGCAATTGATTCTTTCATCTCAAAGCCCATAGCTCTCATTGGAGC  >  NC_007793/2100180‑2100365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑