Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,099,259	G→A	A683A (GCC→GCT)	SAUSA300_RS10590 ←	phage tail tape measure protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,099,259	0	G	A	92.9%	26.9 / ‑5.4	15	A683A (GCC→GCT)	SAUSA300_RS10590	phage tail tape measure protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (0/13);  total (0/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

AGAAGAACTTAATTAAACCTAGTATGATATTTAAAGTTCCTTGTATCACACCTTTTATATTCTCCCAAGTACTGACAATCAAGGCTTTAACCGCCGGCCAAATAAATTGCATCACTTGCCAG  >  NC_007793/2099177‑2099298                                                                                   |                                        agaagaACTTAATTAAACCTAGTATGATTTTTAAAGCTCCTTGTATCACCCCTTTTATATTCTCCCAAGTACTGACAATTAAAGCTTTAACCGCCGGCCa                        <  1:404795/100‑1 (MQ=255) agaagaACTTAATTAAACCTAGTATGATATTTAAAGCTCCTTGTATCACCCCTTTTATATTCTCCCAAGTACTGACAATTAAAGCTTTAACCGCCGGCCa                        <  1:4558191/100‑1 (MQ=255) agaagaACTTAATTAAACCTAGTATGATATTTAAAGCTCCTTGTATCACACCTTTTATATTCTCCCAAGTACTGACAATTAAAGCTTTAACCGCCGGCCa                        <  1:2100570/100‑1 (MQ=255) agaagaACTTAATTAAACCTAGTATGATATTTAAAGCTCCTTGTATCACACCTTTTATATTCTCCCAAGTACTGACAATTAAAGCTTTAACCGCCGGCCa                        <  1:5442746/100‑1 (MQ=255) agaagaACTTAATTAAACCTAGTATGATATTTAAAGCTCCTTGTATCACACCTTTTATATTCTCCCAAGTACTGACAATTAAAGCTTTAACCGCCGGCCa                        <  1:692348/100‑1 (MQ=255)           aattaaACCTAGTATGATATTTAAAGCTCCTTGTATCACACCTTTTATATTCTCCCAAGTACTGACAATTAAAGCTTTAACCGCCGGCCAAATAAATTgg              <  1:59831/100‑2 (MQ=255)           aattaaACCTAGTATGATATTTAAAGCTCCTGGTATCACCCCTTTTATATTCCCCCAAGTACTGACAATTAAAGCTTTAACCGCCGGCCAAATAAATTGc              <  1:4338327/100‑1 (MQ=255)              taaACCTAGTATGATATTTAAAGCTCCTTGTATCACCCCTTTTATATTCTCCCAAGTACTGACAATTAAAGCTTTAACCGCCGGCCAAATAAATTGCATc           <  1:4969312/100‑1 (MQ=255)              aaagccTAGTATGATATTTAAAGCCCCTTGTATCACCCCTTTTATATTCTCCCAAGTACGGAAAATTAAAGCTTTAACCGCCGGCCAAATAAATTGCATc           <  1:2119454/96‑1 (MQ=255)                     aGTTTGATATTTAAAGCTCCTTGTTTCACCCCTTTTATATTTGCCCAAGTACTGACAATTAAAGCTTTAACCGCCGGCCAAATAAATTGCATCACGTGcc    <  1:2865104/100‑1 (MQ=255)                     aGTATGATATTTAAAGCTCCTTGTATCACCCCTTTTATATTCTCCCAAGTACTGACAATAAAAGCTTTAACCGCCGGCCAAATAAATTGCATCACTTGcc    <  1:5736643/100‑1 (MQ=255)                     aGTATGATATTTAAAGCTCCTTGTATCACACCTTTTATATTCTCCCAAGTACTGACAATTAAAGCTTTAACCGCCGGCCAAATAAATTGCATCACTTGcc    <  1:2594895/100‑1 (MQ=255)                     aGTATGATATTTAAAGCTCCTTGAATCACACCGTTTATATTCTCCCAATTACTGACAGTTAACCCTTTAACCGCCGGCCAAATAAATTGCATCACTTGcc    <  1:4555894/100‑1 (MQ=255)                      gTATGATTTTTAAAGCTCCTGGTATCACACCTTTTAGATTCTCCCAAGTTCGGACAATTAAGCCTTAAACCGCCGGCCAAATAAATTGCATCAATTGACa   <  1:206351/100‑1 (MQ=255)                       tATGATATTAAAAGCCCCGTATGCCACTCCTTTTTTATTCCCCCAATTACTGACAATTAAAGCTTAAACCGCCGGCCAAATAAATGGCATCACTTGCCaa  <  1:4796665/100‑2 (MQ=255)                                                                                   |                                        AGAAGAACTTAATTAAACCTAGTATGATATTTAAAGTTCCTTGTATCACACCTTTTATATTCTCCCAAGTACTGACAATCAAGGCTTTAACCGCCGGCCAAATAAATTGCATCACTTGCCAG  >  NC_007793/2099177‑2099298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑