Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,099,213	T→C	T699A (ACT→GCT)	SAUSA300_RS10590 ←	phage tail tape measure protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,099,213	0	T	C	100.0%	89.4 / NA	36	T699A (ACT→GCT)	SAUSA300_RS10590	phage tail tape measure protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/18);  total (18/18)

AATAATCGCGTCCCAAACTCCTCGCCAATCTCCAGTAAATAAACTTGAGAAGAACTTAATTAAACCTAGTATGATATTTAAAGTTCCTTGTATCACACCTTTTATATTCTCCCAAGTACTGACAATCAAGGCTTTAACCGCCGGCCAAATAAATTGCATCACTTGCCAG  >  NC_007793/2099130‑2099298                                                                                    |                                                                                      aacaatCGCATCCCAAACTCCTCGCCAATCTCCAGTAAATAAACTTGAGAAGAACTTAATTAAACCTAGTATGATATTTAAAGCTCCTTGTATCACACCt                                                                       >  1:4784814/4‑100 (MQ=255)         catCCCAAACTCCTCGCCAATCTCCAGTAAATAAACTTGAGAAGAACTTAATTAAACCTAGTATGATATTTAAAGCTCCTTGTATCACACCTTTTATAtt                                                               >  1:3767459/3‑100 (MQ=255)                   tcttcGCTAATCTCCAGTAAATAAACTTGAGAAGAACTTAATTAAACCTAGTATGATATTTAAAGCTCCTTGTATCACACCTTTTATATTCTCCCagcgg                                                     <  1:378595/97‑5 (MQ=255)                    tcccGCCAATCTCCAGTAAATAAACTTGAGAAGAACTTAATTAAACCTAGTATGATATTTAAAGCTCCTTGTATCACACCTTTTATATTCTCCCAAGTAc                                                    <  1:3365057/97‑1 (MQ=255)                      tcGCCAATCTCCAGTAAATAAACTTGAGAAGAACTTAATTAAACCTAGTATGATATTTAAAGCTCCTTGTATCACACCTTTTATATTCTCCCAAGTACTg                                                  <  1:2761202/100‑1 (MQ=255)                               tcCAGTAAATAAACTTGAGAAGAACTTAATTAAACCTAGTATGATATTTAAAGCTCCTTGTATCACACCTTTTATATTCTCCCAAGTACTGACAATTaaa                                         >  1:1341591/1‑99 (MQ=255)                                ccAGTAAATAAACTTGAGAAGAACTTAATTAAACCTAGTATGATATTTAAAGCTCCTTGTATCACACCTTTTATATTCTCCCAAGTACTGACAATTaaag                                        >  1:1556305/1‑98 (MQ=255)                                ccAGTAAATAAACTTGAGAAGAACTTAATTAAACCTAGTATGATATTTAAAGCTCCTTGTATCACACCTTTTATATTCTCCCAAGTACTGACAATTaaag                                        >  1:5381681/1‑98 (MQ=255)                                ccAGTAAATAAACTTGAGAAGAACTTAATTAAACCTAGTATGATATTTAAAGCTCCTTGTATCACACCTTTTATATTCTCCCAAGTACTGACAATTaaag                                        >  1:1135083/1‑98 (MQ=255)                                ccAGTAAATAAACTTGAGAAGAACTTAATTAAACCTAGTATGATATTTAAAGCTCCTTGTATCACACCTTTTATATTCTCCCAAGTACTGACAATTaaag                                        >  1:4600414/1‑98 (MQ=255)                                ccAGTAAATAAACTTGAGAAGAACTTAATTAAACCTAGTATGATATTTAAAGCTCCTTGTATCACACCTTTTATATTCTCCCAAGTACTGACAATTaaag                                        >  1:5574394/1‑98 (MQ=255)                                ccAGTAAATAAACTTGAGAAGAACTTAATTAAACCTAGTATGATATTTAAAGCTCCTTGTATCACACCTTTTATATTCTCCCAAGTACTGACAATTaaag                                        >  1:4311291/1‑98 (MQ=255)                                ccAGTAAATAAACTTGAGAAGAACTTAATTAAACCTAGTATGATATTTAAAGCTCCTTGTATCACACCTTTTATATTCTCCCAAGTACTGACAATTaaag                                        >  1:1473503/1‑98 (MQ=255)                                ccAGTAAATAAACTTGAGAAGAACTTAATTAAACCTAGTATGATATTTAAAGCTCCTTGTATCACACCTTTTATATTCTCCCAAGTACTGACAATTaaag                                        >  1:3528310/1‑98 (MQ=255)                                ccAGTAAATAAACTTGAGAAGAACTTAATTAAACCTAGTATGATATTTAAAGCTCCTTGTATCACACCTTTTATATTCTCCCAAGTACTGACAATTaaag                                        >  1:1758991/1‑98 (MQ=255)                                ccAGTAAATAAACTTGAGAAGAACTTAATTAAACCTAGTATGATATTTAAAGCTCCTTGTATCACACCTTTTATATTCTCCCAAGTACTGACAATTaaag                                        >  1:273980/1‑98 (MQ=255)                                ccAGTAAATAAACTTGAGAAGAACTTAATTAAACCTAGTATGATATTTAAAGCTCCTTGTATCACACCTTTTATATTCTCCCAAGTACTGACAATTaaag                                        >  1:84574/1‑98 (MQ=255)                                ccAGTAAATAAACTTGAGAAGAACTTAATTAAACCTAGTATGATATTTAAAGCTCCTTGTATCACACCTTTTATATTCTCCCAAGTACTGACAATTaaag                                        >  1:2561875/1‑98 (MQ=255)                                ccAGTAAATAAACTTGAGAAGAACTTAATTAAACCTAGTATGATATTTAAAGCTCCTTGTATCACACCTTTTATATTCTCCCAAGTACTGACAATTaaag                                        >  1:2232645/1‑98 (MQ=255)                                ccAGTAAATAAACTTGAGAAGAACTTAATTAAACCTAGTATGATATTTAAAGCTCCTTGTATCACACCTTTTATATTCTCCCAAGTACTGACAATTaaag                                        >  1:222272/1‑98 (MQ=255)                                ccAGTAAATAAACTTGAGAAGAACTTAATTAAACCTAGTATGATATTTAAAGCTCCTTGTATCACACCTTTTATATTCTCCCAAGTACTGACAATTaaag                                        >  1:857295/1‑98 (MQ=255)                                                agaagaACTTAATTAAACCTAGTATGATTTTTAAAGCTCCTTGTATCACCCCTTTTATATTCTCCCAAGTACTGACAATTAAAGCTTTAACCGCCGGCCa                        <  1:404795/100‑1 (MQ=255)                                                agaagaACTTAATTAAACCTAGTATGATATTTAAAGCTCCTTGTATCACCCCTTTTATATTCTCCCAAGTACTGACAATTAAAGCTTTAACCGCCGGCCa                        <  1:4558191/100‑1 (MQ=255)                                                agaagaACTTAATTAAACCTAGTATGATATTTAAAGCTCCTTGTATCACACCTTTTATATTCTCCCAAGTACTGACAATTAAAGCTTTAACCGCCGGCCa                        <  1:692348/100‑1 (MQ=255)                                                agaagaACTTAATTAAACCTAGTATGATATTTAAAGCTCCTTGTATCACACCTTTTATATTCTCCCAAGTACTGACAATTAAAGCTTTAACCGCCGGCCa                        <  1:2100570/100‑1 (MQ=255)                                                agaagaACTTAATTAAACCTAGTATGATATTTAAAGCTCCTTGTATCACACCTTTTATATTCTCCCAAGTACTGACAATTAAAGCTTTAACCGCCGGCCa                        <  1:5442746/100‑1 (MQ=255)                                                          aattaaACCTAGTATGATATTTAAAGCTCCTTGTATCACACCTTTTATATTCTCCCAAGTACTGACAATTAAAGCTTTAACCGCCGGCCAAATAAATTgg              <  1:59831/100‑2 (MQ=255)                                                          aattaaACCTAGTATGATATTTAAAGCTCCTGGTATCACCCCTTTTATATTCCCCCAAGTACTGACAATTAAAGCTTTAACCGCCGGCCAAATAAATTGc              <  1:4338327/100‑1 (MQ=255)                                                             taaACCTAGTATGATATTTAAAGCTCCTTGTATCACCCCTTTTATATTCTCCCAAGTACTGACAATTAAAGCTTTAACCGCCGGCCAAATAAATTGCATc           <  1:4969312/100‑1 (MQ=255)                                                             aaagccTAGTATGATATTTAAAGCCCCTTGTATCACCCCTTTTATATTCTCCCAAGTACGGAAAATTAAAGCTTTAACCGCCGGCCAAATAAATTGCATc           <  1:2119454/96‑1 (MQ=255)                                                                    aGTTTGATATTTAAAGCTCCTTGTTTCACCCCTTTTATATTTGCCCAAGTACTGACAATTAAAGCTTTAACCGCCGGCCAAATAAATTGCATCACGTGcc    <  1:2865104/100‑1 (MQ=255)                                                                    aGTATGATATTTAAAGCTCCTTGTATCACCCCTTTTATATTCTCCCAAGTACTGACAATAAAAGCTTTAACCGCCGGCCAAATAAATTGCATCACTTGcc    <  1:5736643/100‑1 (MQ=255)                                                                    aGTATGATATTTAAAGCTCCTTGTATCACACCTTTTATATTCTCCCAAGTACTGACAATTAAAGCTTTAACCGCCGGCCAAATAAATTGCATCACTTGcc    <  1:2594895/100‑1 (MQ=255)                                                                    aGTATGATATTTAAAGCTCCTTGAATCACACCGTTTATATTCTCCCAATTACTGACAGTTAACCCTTTAACCGCCGGCCAAATAAATTGCATCACTTGcc    <  1:4555894/100‑1 (MQ=255)                                                                     gTATGATTTTTAAAGCTCCTGGTATCACACCTTTTAGATTCTCCCAAGTTCGGACAATTAAGCCTTAAACCGCCGGCCAAATAAATTGCATCAATTGACa   <  1:206351/100‑1 (MQ=255)                                                                      tATGATATTAAAAGCCCCGTATGCCACTCCTTTTTTATTCCCCCAATTACTGACAATTAAAGCTTAAACCGCCGGCCAAATAAATGGCATCACTTGCCaa  <  1:4796665/100‑2 (MQ=255)                                                                                    |                                                                                      AATAATCGCGTCCCAAACTCCTCGCCAATCTCCAGTAAATAAACTTGAGAAGAACTTAATTAAACCTAGTATGATATTTAAAGTTCCTTGTATCACACCTTTTATATTCTCCCAAGTACTGACAATCAAGGCTTTAACCGCCGGCCAAATAAATTGCATCACTTGCCAG  >  NC_007793/2099130‑2099298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑