Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,099,139	G→A	D723D (GAC→GAT)	SAUSA300_RS10590 ←	phage tail tape measure protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,099,139	0	G	A	100.0%	18.9 / NA	8	D723D (GAC→GAT)	SAUSA300_RS10590	phage tail tape measure protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/3);  total (5/3)

AAGTACCTAACAACGCCAAGTATTTTGCCTACAAACCATAATTGAATTAAATTCCATATTAATTGAACGACTCCTTTAAGAATCATAATAATCGCGTCCCAAACTCCTCGCCAATCTCCAGTAAATAAACTTGAGAAGAACTTAATTAAACCTAGTATGATATTTAAAGTTCCTTGTATCACACCT  >  NC_007793/2099044‑2099229                                                                                                |                                                                                           aaGTACCTAACAACGCCAAGTATTTTGCCTACAAACCATAATTGAATTAAATTCCATATTAATTGAACGACTCCTTTAAGAATCATAACAATCGCATccc                                                                                        >  1:923580/1‑100 (MQ=255)  aGTACCTAACAACGCCAAGTATTTTGCCTACAAACCATAATTGAATTAAATTCCATATTAATTGAACGACTCCTTTAAGAATCATAACAATCGCATCCCa                                                                                       <  1:3993873/100‑1 (MQ=255)  aGTACCTAACAACGCCAAGTATTTTGCCTACAAACCATAATTGAATTAAATTCCATATTAATTGAACGACTCCTTTAAGAATCATAACAATCGCATCCCa                                                                                       <  1:4501465/100‑1 (MQ=255)                               aCAAACCATAATTGAATTAAATTCCATATTAATTGAACGACTCCTTTAAGAATCATAACAATCGCATCCCAAACTCCTCGCCAATCTCCAGTAAATAAAc                                                          >  1:5693982/1‑100 (MQ=255)                                                 aaaTTCCATATTAATTGAACGACTCCTTTAAGAATCATAACAATCGCATCCCAAACTCCTCGCCAATCTCCAGTAAATAAACTTGAGAAGAACttaatta                                        >  1:264446/1‑100 (MQ=255)                                                              ttttccgatcTCCTTTAAGAATCATAACAATCGCATCCCAAACTCCTCGCCAATCTCCAGTAAATAAACTTGAGAAGAACTTAATTAAACCTAGTATGat                           <  1:2394033/91‑1 (MQ=255)                                                                    ggACTCCTTTAAGAATCATAACAATCGCATCCCAAACTCCTCGCCAATCTCCAGTAAATAAACTTGAGAAGAACTTAATTAAACCTAGTATGATATTTaa                     >  1:682001/2‑100 (MQ=255)                                                                                       aacaatCGCATCCCAAACTCCTCGCCAATCTCCAGTAAATAAACTTGAGAAGAACTTAATTAAACCTAGTATGATATTTAAAGCTCCTTGTATCACACCt  >  1:4784814/4‑100 (MQ=255)                                                                                                |                                                                                           AAGTACCTAACAACGCCAAGTATTTTGCCTACAAACCATAATTGAATTAAATTCCATATTAATTGAACGACTCCTTTAAGAATCATAATAATCGCGTCCCAAACTCCTCGCCAATCTCCAGTAAATAAACTTGAGAAGAACTTAATTAAACCTAGTATGATATTTAAAGTTCCTTGTATCACACCT  >  NC_007793/2099044‑2099229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑