Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,099,132	T→C	I726V (ATT→GTT)	SAUSA300_RS10590 ←	phage tail tape measure protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,099,132	0	T	C	100.0%	13.2 / NA	7	I726V (ATT→GTT)	SAUSA300_RS10590	phage tail tape measure protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/3);  total (4/3)

AAGTACCTAACAACGCCAAGTATTTTGCCTACAAACCATAATTGAATTAAATTCCATATTAATTGAACGACTCCTTTAAGAATCATAATAATCGCGTCCCAAACTCCTCGCCAATCTCCAGTAAATAAACTTGAGAAGAACTTAATTAAACCTAGTATGATATTTAA  >  NC_007793/2099044‑2099210                                                                                         |                                                                               aaGTACCTAACAACGCCAAGTATTTTGCCTACAAACCATAATTGAATTAAATTCCATATTAATTGAACGACTCCTTTAAGAATCATAACAATCGCATccc                                                                     >  1:923580/1‑100 (MQ=255)  aGTACCTAACAACGCCAAGTATTTTGCCTACAAACCATAATTGAATTAAATTCCATATTAATTGAACGACTCCTTTAAGAATCATAACAATCGCATCCCa                                                                    <  1:3993873/100‑1 (MQ=255)  aGTACCTAACAACGCCAAGTATTTTGCCTACAAACCATAATTGAATTAAATTCCATATTAATTGAACGACTCCTTTAAGAATCATAACAATCGCATCCCa                                                                    <  1:4501465/100‑1 (MQ=255)                               aCAAACCATAATTGAATTAAATTCCATATTAATTGAACGACTCCTTTAAGAATCATAACAATCGCATCCCAAACTCCTCGCCAATCTCCAGTAAATAAAc                                       >  1:5693982/1‑100 (MQ=255)                                                 aaaTTCCATATTAATTGAACGACTCCTTTAAGAATCATAACAATCGCATCCCAAACTCCTCGCCAATCTCCAGTAAATAAACTTGAGAAGAACttaatta                     >  1:264446/1‑100 (MQ=255)                                                              ttttccgatcTCCTTTAAGAATCATAACAATCGCATCCCAAACTCCTCGCCAATCTCCAGTAAATAAACTTGAGAAGAACTTAATTAAACCTAGTATGat        <  1:2394033/91‑1 (MQ=255)                                                                    ggACTCCTTTAAGAATCATAACAATCGCATCCCAAACTCCTCGCCAATCTCCAGTAAATAAACTTGAGAAGAACTTAATTAAACCTAGTATGATATTTaa  >  1:682001/2‑100 (MQ=255)                                                                                         |                                                                               AAGTACCTAACAACGCCAAGTATTTTGCCTACAAACCATAATTGAATTAAATTCCATATTAATTGAACGACTCCTTTAAGAATCATAATAATCGCGTCCCAAACTCCTCGCCAATCTCCAGTAAATAAACTTGAGAAGAACTTAATTAAACCTAGTATGATATTTAA  >  NC_007793/2099044‑2099210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑