Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,098,575	T→G	E911D (GAA→GAC)	SAUSA300_RS10590 ←	phage tail tape measure protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,098,575	0	T	G	100.0%	28.9 / NA	14	E911D (GAA→GAC)	SAUSA300_RS10590	phage tail tape measure protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (4/10);  total (4/10)

TAACTAATCTTGTAGTAGTATGTGTGTGTTCTGTACCAGTGTGTAACTTAGGTATTTTATCCATTCCCAGCTTACCACCGACCCAGTTTAAACCTTCAATTAATTTATTAAGTCCTTTTTTAATAGCATCTACCATACCGCCGATATGACTTTTAATTTTGCTGATAA  >  NC_007793/2098481‑2098648                                                                                               |                                                                          tAACTAATCTTGTAGTAGTATGTGTGTGCTCTGTACCAGTATGTAACTTAGGTATTTTATCCATGCCCAACTTACCACCGACCCAGTTTAAACCGTCgat                                                                      >  1:652432/1‑100 (MQ=255)              agtagtaTGTGTGTGCTCTGTACCAGTATGTAACTTAGGTATTTTATCCATGCCCAACTTACCACCGACCCAGTTTAAACCGTCGATTAATTTATTAAGt                                                         >  1:4791657/1‑100 (MQ=255)                 agtaTGTGTGTGCTCTGTACCAGTATGTAACTTAGGTATTTTATCCATGCCCAACTTACCACCGACCCAGTTTAAACCGTCGATTAATTTATTAAGTCCt                                                      >  1:2673312/1‑100 (MQ=255)                              tCTGTGCCAGTATGTAACTTAGGTATTTTATCCATGCCCAACTTACCACCGACCCAGTTTAAACCGTCGATTAATTTATTAAGTCCTTTTTTAATAGCag                                         <  1:1437857/100‑2 (MQ=255)                              tCTGTACCAGTATGTAACTTAGGTATTTTATCCATGCCCAACTTACCACCGACCCAGTTTAAACCGTCGATTAATTTATTAAGTCCTTTTTTAATAGCAt                                         <  1:2163489/100‑1 (MQ=255)                               cTGTACCAGTATGTAACTTAGGTATTTTATCCATGCCCAACTTACCACCGACCCAGTTTAAACCGTCGATTAATTTATTAAGTCCTTTTTTAATAGCAtg                                        <  1:5730399/100‑2 (MQ=255)                                   aCCAGTATGTAACTTAGGTATTTTATCCATGCCCAACTTACCACCGACCCAGTTTAAACCGTCGATTAATTTATTAAGTCCTTTTTTAATAGCATCTAcc                                    <  1:702576/100‑1 (MQ=255)                                    ccAGTATGTAACTTAGGCATTTTATCCATGCCCAACTTACCACCGACCCAGTTTAAACCGTCGATTAATTTATTAAGTCCTTTTTTAATAGCATCTACCa                                   <  1:3354450/100‑1 (MQ=255)                                            tAACTTAGGTATTTTATCCATGCCCAACTTACCACCGACCCAGTTTAAACCGTCGATTAATTTATTAAGTCCTTTTTTAATAGCATCTACCATAccgccg                           <  1:1089214/100‑1 (MQ=255)                                             aaCTTAGGTATTTTATCCATGCCCAACTTACCACCGACCCAGTTTAAACCGTCGATTAATTTATTAAGTCCTTTTTTAATAGCATCTACCAtcaccaaaa                          <  1:4845513/100‑9 (MQ=255)                                                       ttcgaTCTATGCCCAACTTACCACCGACCCAGTTTAACCCGTCGATTAATTTATTAAGTCCTTTTTTAATAGCATCTCCCATACCGCCGATATGACtttt                <  1:4818241/96‑1 (MQ=255)                                                                 gcccAACTTACCACCGACCCAGTTTAAACCGTCGATTAATTTATTAAGTCCTTTTTTAATAGCATCTACCATACCGCCGATATGACTTTTAATTTTGCTg      >  1:3981688/2‑100 (MQ=255)                                                                   ccAACTTACCACCGACCCAGTTTAAACCGTCGATTAATTTATTAAGTCCTTTTTTAATAGCACCTACCATACCGCCGATATGACTTTTAATTTTGCTGat    <  1:2975132/100‑1 (MQ=255)                                                                     aacTTACCACCGCCCCAGTTTAAACGGTCGATTAATTGATTAAGTCCTTTTAAAATAGAGTCTCCCATACCGCAGATATGACTTTTAATTTTGCTGatag  <  1:4803321/98‑2 (MQ=255)                                                                                               |                                                                          TAACTAATCTTGTAGTAGTATGTGTGTGTTCTGTACCAGTGTGTAACTTAGGTATTTTATCCATTCCCAGCTTACCACCGACCCAGTTTAAACCTTCAATTAATTTATTAAGTCCTTTTTTAATAGCATCTACCATACCGCCGATATGACTTTTAATTTTGCTGATAA  >  NC_007793/2098481‑2098648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑