Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,098,550	G→A	L920L (CTG→TTG)	SAUSA300_RS10590 ←	phage tail tape measure protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,098,550	0	G	A	100.0%	56.7 / NA	21	L920L (CTG→TTG)	SAUSA300_RS10590	phage tail tape measure protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/17);  total (4/17)

CCGCGCAATCTTACCGTTCTTAACTAATCTTGTAGTAGTATGTGTGTGTTCTGTACCAGTGTGTAACTTAGGTATTTTATCCATTCCCAGCTTACCACCGACCCAGTTTAAACCTTCAATTAATTTATTAAGTCCTTTTTTAATAGCATCTACCATACCGCCGATATGACTTTTAATTTTGCTGAT  >  NC_007793/2098461‑2098646                                                                                          |                                                                                                 tcttccgaTCTTACCGTTCTTAACTAATCTTGTAGTAGTATGTGTGTGCTCTGTACCAGTATGTAACTTAGGTATTTTATCCATGCCCAACTTACCACCg                                                                                        <  1:604771/93‑1 (MQ=255)     tgtgctcttccGATCTTAACCAATCTTGTAGTAGTATGTGTGTGCTCTGTACCAGTATGTAACTTAGGTATTTTATCCATGCCCAACTTACCACCGAccc                                                                                    <  1:140138/91‑1 (MQ=255)     gcAATCTTACCGTTCTTAACTAATCTTGTAGTAGTATGTGTGTGCTCTGTACCAGTATGTAACTTAGGTATTTTATCCATGCCCAACTTACCACCGAccc                                                                                    <  1:151106/100‑1 (MQ=255)     gcAATCTTACCGTTCTTAACTAATCTTGTAGTAGTATGTGTGTGCTCTGTACCAGTATGTAACTTAGGTATTTTATCCATGCCCAACTTACCACCGAccc                                                                                    <  1:2644946/100‑1 (MQ=255)      cAATCTTACCGTTCTTAACTAATCTTGTAGTAGTATGTGTGTGCTCTGTACCAGTATGTAACTTAGGTATTTTATCCATGCCCAACTTACCACCGACCCa                                                                                   <  1:267046/100‑1 (MQ=255)         ctcttccGATCTTATTTAATCTTGTAGTAGTATGTGTGTGCTCTGTACCAGTATGTAACTTAGGTATTTTATCCATGCCCAACTTACCACCGACCCAGtc                                                                                <  1:919333/95‑2 (MQ=255)         ctcttccGATCTTAACTTATCTTGTAGTAGTATGTGTGTGCTCTGTACCAGTATGTAACTTAGGTATTTTATCCATGCCCAACTTACCACCGACCCAgct                                                                                <  1:3562694/95‑3 (MQ=255)          cTTACCGTTCTTAACTAATCTTGTAGTAGTATGTGTGTGCTCTGTACCAGTATGTAACTTAGGTATTTTATCCATGCCCAACTTACCACCGACCCAGttt                                                                               <  1:2216768/100‑1 (MQ=255)                     tAACTAATCTTGTAGTAGTATGTGTGTGCTCTGTACCAGTATGTAACTTAGGTATTTTATCCATGCCCAACTTACCACCGACCCAGTTTAAACCGTCgat                                                                    >  1:652432/1‑100 (MQ=255)                                  agtagtaTGTGTGTGCTCTGTACCAGTATGTAACTTAGGTATTTTATCCATGCCCAACTTACCACCGACCCAGTTTAAACCGTCGATTAATTTATTAAGt                                                       >  1:4791657/1‑100 (MQ=255)                                     agtaTGTGTGTGCTCTGTACCAGTATGTAACTTAGGTATTTTATCCATGCCCAACTTACCACCGACCCAGTTTAAACCGTCGATTAATTTATTAAGTCCt                                                    >  1:2673312/1‑100 (MQ=255)                                                  tCTGTGCCAGTATGTAACTTAGGTATTTTATCCATGCCCAACTTACCACCGACCCAGTTTAAACCGTCGATTAATTTATTAAGTCCTTTTTTAATAGCag                                       <  1:1437857/100‑2 (MQ=255)                                                  tCTGTACCAGTATGTAACTTAGGTATTTTATCCATGCCCAACTTACCACCGACCCAGTTTAAACCGTCGATTAATTTATTAAGTCCTTTTTTAATAGCAt                                       <  1:2163489/100‑1 (MQ=255)                                                   cTGTACCAGTATGTAACTTAGGTATTTTATCCATGCCCAACTTACCACCGACCCAGTTTAAACCGTCGATTAATTTATTAAGTCCTTTTTTAATAGCAtg                                      <  1:5730399/100‑2 (MQ=255)                                                       aCCAGTATGTAACTTAGGTATTTTATCCATGCCCAACTTACCACCGACCCAGTTTAAACCGTCGATTAATTTATTAAGTCCTTTTTTAATAGCATCTAcc                                  <  1:702576/100‑1 (MQ=255)                                                        ccAGTATGTAACTTAGGCATTTTATCCATGCCCAACTTACCACCGACCCAGTTTAAACCGTCGATTAATTTATTAAGTCCTTTTTTAATAGCATCTACCa                                 <  1:3354450/100‑1 (MQ=255)                                                                tAACTTAGGTATTTTATCCATGCCCAACTTACCACCGACCCAGTTTAAACCGTCGATTAATTTATTAAGTCCTTTTTTAATAGCATCTACCATAccgccg                         <  1:1089214/100‑1 (MQ=255)                                                                 aaCTTAGGTATTTTATCCATGCCCAACTTACCACCGACCCAGTTTAAACCGTCGATTAATTTATTAAGTCCTTTTTTAATAGCATCTACCAtcaccaaaa                        <  1:4845513/100‑9 (MQ=255)                                                                           ttcgaTCTATGCCCAACTTACCACCGACCCAGTTTAACCCGTCGATTAATTTATTAAGTCCTTTTTTAATAGCATCTCCCATACCGCCGATATGACtttt              <  1:4818241/96‑1 (MQ=255)                                                                                     gcccAACTTACCACCGACCCAGTTTAAACCGTCGATTAATTTATTAAGTCCTTTTTTAATAGCATCTACCATACCGCCGATATGACTTTTAATTTTGCTg    >  1:3981688/2‑100 (MQ=255)                                                                                       ccAACTTACCACCGACCCAGTTTAAACCGTCGATTAATTTATTAAGTCCTTTTTTAATAGCACCTACCATACCGCCGATATGACTTTTAATTTTGCTGat  <  1:2975132/100‑1 (MQ=255)                                                                                          |                                                                                                 CCGCGCAATCTTACCGTTCTTAACTAATCTTGTAGTAGTATGTGTGTGTTCTGTACCAGTGTGTAACTTAGGTATTTTATCCATTCCCAGCTTACCACCGACCCAGTTTAAACCTTCAATTAATTTATTAAGTCCTTTTTTAATAGCATCTACCATACCGCCGATATGACTTTTAATTTTGCTGAT  >  NC_007793/2098461‑2098646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑