Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	47,722	G→T	I390I (ATC→ATA)	SAUSA300_RS00205 ←	recombinase RecA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	47,722	0	G	T	100.0%	44.7 / NA	17	I390I (ATC→ATA)	SAUSA300_RS00205	recombinase RecA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (1/16);  total (1/16)

TCGTAATGTGAATGGTTTCAATATAGGGGTACAATATGTTTAACGTGAAACGTTTTTGAATAATATTTTGAATGATGTGTTGTATTTGATGCCCATTGATAGATGTAGTGCGTTGCGGTTGTTGACGTAATGATTGTGTTTGCTCTCTGAACGTTTCTGCATCAATGATGCCTTGTGCCA  >  NC_007793/47625‑47804                                                                                                  |                                                                                   tCGTAATGTGAATGGTTTCAATATAGGGGTACAATATGTTTAACGTGAAACGTTTTTGAATAATATTTTGAATGGTGTGTTGTATTTGATGCCCATTTAt                                                                                  <  1:1573827/100‑1 (MQ=255)       tgtgAATGGTTTCAATATAGGGGTACAATATGTTTAACGTGAAACGTTTTTGAATAATATTTTGAATGGTGTGTTGTATTTGATGCCCATTTATAGATgc                                                                            <  1:4690002/100‑2 (MQ=255)         tgAATGGTTTCAATATAGGGGTACAATATGTTTAACGTGAAACGTTTTCGAATAATATTTTGAATGGTGTGTTGTATTTGATGCCCATTTATAGATGTAg                                                                          <  1:5470402/100‑1 (MQ=255)            aTGGTTTCAATATAGGGGTACAATATGTTTAACGTGAAACGTTTTTGAATAATATTTTGAATGGTGTGTTGTATTTGATGCCCATTTATAGATGTAGTgt                                                                       <  1:3928103/100‑2 (MQ=255)               cttccgatctaGGGGTACAATATGTTTAACGTGAAACGTTTTTGAATAATATTTTGAATGGTGTGTTGTATTTGATGCCCATTTATAGATGTAGTGCGtt                                                                    <  1:3393525/91‑1 (MQ=255)                ttccgatctaGGGGTACAATATGTTTAACGTGAAACGTTTTTGAATAATATTTTGAATGGTGTGTTGTATTTGATGCCCATTTATAGATGTAGTGCGTTg                                                                   <  1:5421649/92‑1 (MQ=255)                    aaTATAGGGGTACAATATGTTTAACGTGAAACGTTTTTGAATAATATTTTGAATGGTGTGTTGTATTTGATGCCCATTTATAGATGTAGTGCGTTGCGgt                                                               <  1:3470889/100‑1 (MQ=255)                    aaTATAGGGGTACAATATGTTTAACGTGAAACGTTTTTGAATAATATTTTGAATGGTGTGTTGTATTTGATGCCCATTTATAGATGTAGTGCGTTGCGgt                                                               <  1:5107862/100‑1 (MQ=255)                         aGGGTTACAATATGTTTAACGTGAAACGTTTTTGAATAATATTTTGAATGGTGTGTTGTATTTGATGCCCATTTATAGATGTAGTGCGTTGCGgttgtag                                                          <  1:5292604/100‑3 (MQ=255)                         aGGGGTACAATATGTTTAACGTGAAACGTTTTTGAATAATATTTTGAATGGTGTGTTGTATTTGATGCCCATTTATAGATGTAGTGCGTTGCGGttgttg                                                          <  1:4720931/100‑1 (MQ=255)                          tgggTACAATATGTTTAACGTGAAACGTTTTTGAATAATATTTTGAATGGTGTGTTGTATTTGATGCCCATTTATAGATGTAGTGCGTTGcagatcggaa                                                         >  1:1847418/2‑91 (MQ=255)                              tACAATATGTTTAACGTGAAACGTTTTTGAATAATATTTTGAATGGTGTGTTGTATTTGATGCCCATTTATAGATGTAGTGCGTTGCGGTTGTTGACGTa                                                     <  1:5386802/100‑1 (MQ=255)                              tACAATATGTTTAACGTGAAACGTTTTTGAATAATATTTTGAATGGTGTGTTGTATTTGATGCCCATTTATAGATGTAGTGCGTTGCGGTTGTTGACGTa                                                     <  1:2284147/100‑1 (MQ=255)                                         tAACGTGAAACGTTTTTGAATAATATTTTGAATGGTGTGTTGTATTTGATGCCCATTTATAGATGTAGTGCGTTGCGGTTGTTGACGTAATGATTGCGtt                                          <  1:5513168/100‑1 (MQ=255)                                               gAAACGTTTTTGAATAATATTTTGAATGGTGTGTTGTATTTGATGCCCATTTATAGATGTAGTGCGTTGCGGTTGTTGACGTAATGATTGCGTTTGttct                                    <  1:3276575/100‑1 (MQ=255)                                                                                 tgtATTTGATGCCCATTTATAGATGTAGTGCGTTGCGGTTGTTGACGTAATGATTGCGTTTGTTCTCTGAACGTTTCTGCATCAATGATGCCTTGTGCCa  <  1:587932/100‑1 (MQ=255)                                                                                 cttccgatctGCCCATTTATAGATGTAGTGCGTTGCGGTTGTTGACGTAATGATTGCGTTTGTTCTCTGAACGTTTCTGCATCAATGATGCCTTGTGCCa  <  1:2574857/91‑1 (MQ=255)                                                                                                  |                                                                                   TCGTAATGTGAATGGTTTCAATATAGGGGTACAATATGTTTAACGTGAAACGTTTTTGAATAATATTTTGAATGATGTGTTGTATTTGATGCCCATTGATAGATGTAGTGCGTTGCGGTTGTTGACGTAATGATTGTGTTTGCTCTCTGAACGTTTCTGCATCAATGATGCCTTGTGCCA  >  NC_007793/47625‑47804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑