Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,098,521	G→A	H929H (CAC→CAT)	SAUSA300_RS10590 ←	phage tail tape measure protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,098,521	0	G	A	100.0%	48.5 / NA	18	H929H (CAC→CAT)	SAUSA300_RS10590	phage tail tape measure protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/15);  total (3/15)

TCCACGTCCTTTATCCCCAACCGTAGCGAACGTATCCCGCGCAATCTTACCGTTCTTAACTAATCTTGTAGTAGTATGTGTGTGTTCTGTACCAGTGTGTAACTTAGGTATTTTATCCATTCCCAGCTTACCACCGACCCAGTTTAAACCTTCAATTAATTTATTAAGTCCTTTTTTAATAGCATCTACCA  >  NC_007793/2098425‑2098615                                                                                                 |                                                                                               tCCGCGTCCCTTATCTCCAACTGTAGCGAATGTGTCACGTGCAATCTTACCGTTCTTAACTAATCTTGTAGTAGTATGTGTGTGCTCTGTACCAgtatgt                                                                                             <  1:1354825/100‑1 (MQ=255)                      tgTAGCGAATGTGTCACGTGCAATCTTACCGTTCTTAACTAATCTTGTAGTAGTATGTGTGTGCTCTGTACCAGTATGTAACTTAGGTATTTTATCCAtg                                                                        <  1:4637946/99‑2 (MQ=255)                          gCGAATGTGTCACGTGCAATCTTACCGTTCTTAACTAATCTTGTAGTAGTATGTGTGTGCTCTGTACCAGTATGTAACTTAGGTATTTTATCCATGCCCa                                                                    <  1:4181373/100‑1 (MQ=255)                                     tcttccgaTCTTACCGTTCTTAACTAATCTTGTAGTAGTATGTGTGTGCTCTGTACCAGTATGTAACTTAGGTATTTTATCCATGCCCAACTTACCACCg                                                         <  1:604771/93‑1 (MQ=255)                                         tgtgctcttccGATCTTAACCAATCTTGTAGTAGTATGTGTGTGCTCTGTACCAGTATGTAACTTAGGTATTTTATCCATGCCCAACTTACCACCGAccc                                                     <  1:140138/91‑1 (MQ=255)                                         gcAATCTTACCGTTCTTAACTAATCTTGTAGTAGTATGTGTGTGCTCTGTACCAGTATGTAACTTAGGTATTTTATCCATGCCCAACTTACCACCGAccc                                                     <  1:151106/100‑1 (MQ=255)                                         gcAATCTTACCGTTCTTAACTAATCTTGTAGTAGTATGTGTGTGCTCTGTACCAGTATGTAACTTAGGTATTTTATCCATGCCCAACTTACCACCGAccc                                                     <  1:2644946/100‑1 (MQ=255)                                          cAATCTTACCGTTCTTAACTAATCTTGTAGTAGTATGTGTGTGCTCTGTACCAGTATGTAACTTAGGTATTTTATCCATGCCCAACTTACCACCGACCCa                                                    <  1:267046/100‑1 (MQ=255)                                             ctcttccGATCTTATTTAATCTTGTAGTAGTATGTGTGTGCTCTGTACCAGTATGTAACTTAGGTATTTTATCCATGCCCAACTTACCACCGACCCAGtc                                                 <  1:919333/95‑2 (MQ=255)                                             ctcttccGATCTTAACTTATCTTGTAGTAGTATGTGTGTGCTCTGTACCAGTATGTAACTTAGGTATTTTATCCATGCCCAACTTACCACCGACCCAgct                                                 <  1:3562694/95‑3 (MQ=255)                                              cTTACCGTTCTTAACTAATCTTGTAGTAGTATGTGTGTGCTCTGTACCAGTATGTAACTTAGGTATTTTATCCATGCCCAACTTACCACCGACCCAGttt                                                <  1:2216768/100‑1 (MQ=255)                                                         tAACTAATCTTGTAGTAGTATGTGTGTGCTCTGTACCAGTATGTAACTTAGGTATTTTATCCATGCCCAACTTACCACCGACCCAGTTTAAACCGTCgat                                     >  1:652432/1‑100 (MQ=255)                                                                      agtagtaTGTGTGTGCTCTGTACCAGTATGTAACTTAGGTATTTTATCCATGCCCAACTTACCACCGACCCAGTTTAAACCGTCGATTAATTTATTAAGt                        >  1:4791657/1‑100 (MQ=255)                                                                         agtaTGTGTGTGCTCTGTACCAGTATGTAACTTAGGTATTTTATCCATGCCCAACTTACCACCGACCCAGTTTAAACCGTCGATTAATTTATTAAGTCCt                     >  1:2673312/1‑100 (MQ=255)                                                                                      tCTGTGCCAGTATGTAACTTAGGTATTTTATCCATGCCCAACTTACCACCGACCCAGTTTAAACCGTCGATTAATTTATTAAGTCCTTTTTTAATAGCag        <  1:1437857/100‑2 (MQ=255)                                                                                      tCTGTACCAGTATGTAACTTAGGTATTTTATCCATGCCCAACTTACCACCGACCCAGTTTAAACCGTCGATTAATTTATTAAGTCCTTTTTTAATAGCAt        <  1:2163489/100‑1 (MQ=255)                                                                                       cTGTACCAGTATGTAACTTAGGTATTTTATCCATGCCCAACTTACCACCGACCCAGTTTAAACCGTCGATTAATTTATTAAGTCCTTTTTTAATAGCAtg       <  1:5730399/100‑2 (MQ=255)                                                                                           aCCAGTATGTAACTTAGGTATTTTATCCATGCCCAACTTACCACCGACCCAGTTTAAACCGTCGATTAATTTATTAAGTCCTTTTTTAATAGCATCTAcc   <  1:702576/100‑1 (MQ=255)                                                                                            ccAGTATGTAACTTAGGCATTTTATCCATGCCCAACTTACCACCGACCCAGTTTAAACCGTCGATTAATTTATTAAGTCCTTTTTTAATAGCATCTACCa  <  1:3354450/100‑1 (MQ=255)                                                                                                 |                                                                                               TCCACGTCCTTTATCCCCAACCGTAGCGAACGTATCCCGCGCAATCTTACCGTTCTTAACTAATCTTGTAGTAGTATGTGTGTGTTCTGTACCAGTGTGTAACTTAGGTATTTTATCCATTCCCAGCTTACCACCGACCCAGTTTAAACCTTCAATTAATTTATTAAGTCCTTTTTTAATAGCATCTACCA  >  NC_007793/2098425‑2098615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑