Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	47,699	A→G	I398T (ATC→ACC)	SAUSA300_RS00205 ←	recombinase RecA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	47,699	0	A	G	100.0%	44.4 / NA	19	I398T (ATC→ACC)	SAUSA300_RS00205	recombinase RecA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (3/16);  total (3/16)

TTCCTATAAGATTTTTATCTTTCGTAATGTGAATGGTTTCAATATAGGGGTACAATATGTTTAACGTGAAACGTTTTTGAATAATATTTTGAATGATGTGTTGTATTTGATGCCCATTGATAGATGTAGTGCGTTGCGGTTGTTGACGTAATGATTGTGTTTGCTCT  >  NC_007793/47604‑47770                                                                                                |                                                                        ttCCTATAAGATTTTTATCTTTCGTAATGTGAATGGTTTCAATATAGGGGTACAATATGTTTAACGTGAAACGTTTTTGAATAATATTTTGAATGgtgtc                                                                     <  1:1164491/100‑2 (MQ=255)  tCCTATAAGATTTTTATCTTTCGTAATGTGAATGGTTTCAATATAGGGGTACAATATGTTTAACGTGAAACGTTTTTGAATAATATTTTGAATGGtgtgg                                                                    <  1:4652644/100‑2 (MQ=255)   ccTATAAGATTTTTATCTTTCGTAATGTGAATGGTTTCAATATAGGGGTACAATATGTTTAACGTGAAACGTTTTTGAATAATATTTTGAATGGTGtgtt                                                                   >  1:2084162/1‑100 (MQ=255)   ccTATAAGATTTTTATCTTTCGTAATGTGAATGGTTTCAATATAGGGGTACAATATGTTTAACGTGAAACGTTTTTGAATAATAGTTTGAATGGTGtgtt                                                                   <  1:3827638/100‑1 (MQ=255)                     ttCGTAATGTGAATGGTTTCAATATAGGGGTACAATATGTTTAACGTGAAACGTTTTTGAATAATATTTTGAATGGTGTGTTGTATTTGATGCCCAttta                                                 >  1:2227750/1‑98 (MQ=255)                      tCGTAATGTGAATGGTTTCAATATAGGGGTACAATATGTTTAACGTGAAACGTTTTTGAATAATATTTTGAATGGTGTGTTGTATTTGATGCCCATTTAt                                                <  1:1573827/100‑1 (MQ=255)                            tgtgAATGGTTTCAATATAGGGGTACAATATGTTTAACGTGAAACGTTTTTGAATAATATTTTGAATGGTGTGTTGTATTTGATGCCCATTTATAGATgc                                          <  1:4690002/100‑2 (MQ=255)                              tgAATGGTTTCAATATAGGGGTACAATATGTTTAACGTGAAACGTTTTCGAATAATATTTTGAATGGTGTGTTGTATTTGATGCCCATTTATAGATGTAg                                        <  1:5470402/100‑1 (MQ=255)                                 aTGGTTTCAATATAGGGGTACAATATGTTTAACGTGAAACGTTTTTGAATAATATTTTGAATGGTGTGTTGTATTTGATGCCCATTTATAGATGTAGTgt                                     <  1:3928103/100‑2 (MQ=255)                                    cttccgatctaGGGGTACAATATGTTTAACGTGAAACGTTTTTGAATAATATTTTGAATGGTGTGTTGTATTTGATGCCCATTTATAGATGTAGTGCGtt                                  <  1:3393525/91‑1 (MQ=255)                                     ttccgatctaGGGGTACAATATGTTTAACGTGAAACGTTTTTGAATAATATTTTGAATGGTGTGTTGTATTTGATGCCCATTTATAGATGTAGTGCGTTg                                 <  1:5421649/92‑1 (MQ=255)                                         aaTATAGGGGTACAATATGTTTAACGTGAAACGTTTTTGAATAATATTTTGAATGGTGTGTTGTATTTGATGCCCATTTATAGATGTAGTGCGTTGCGgt                             <  1:3470889/100‑1 (MQ=255)                                         aaTATAGGGGTACAATATGTTTAACGTGAAACGTTTTTGAATAATATTTTGAATGGTGTGTTGTATTTGATGCCCATTTATAGATGTAGTGCGTTGCGgt                             <  1:5107862/100‑1 (MQ=255)                                              aGGGTTACAATATGTTTAACGTGAAACGTTTTTGAATAATATTTTGAATGGTGTGTTGTATTTGATGCCCATTTATAGATGTAGTGCGTTGCGgttgtag                        <  1:5292604/100‑3 (MQ=255)                                              aGGGGTACAATATGTTTAACGTGAAACGTTTTTGAATAATATTTTGAATGGTGTGTTGTATTTGATGCCCATTTATAGATGTAGTGCGTTGCGGttgttg                        <  1:4720931/100‑1 (MQ=255)                                               tgggTACAATATGTTTAACGTGAAACGTTTTTGAATAATATTTTGAATGGTGTGTTGTATTTGATGCCCATTTATAGATGTAGTGCGTTGcagatcggaa                       >  1:1847418/2‑91 (MQ=255)                                                   tACAATATGTTTAACGTGAAACGTTTTTGAATAATATTTTGAATGGTGTGTTGTATTTGATGCCCATTTATAGATGTAGTGCGTTGCGGTTGTTGACGTa                   <  1:2284147/100‑1 (MQ=255)                                                   tACAATATGTTTAACGTGAAACGTTTTTGAATAATATTTTGAATGGTGTGTTGTATTTGATGCCCATTTATAGATGTAGTGCGTTGCGGTTGTTGACGTa                   <  1:5386802/100‑1 (MQ=255)                                                              tAACGTGAAACGTTTTTGAATAATATTTTGAATGGTGTGTTGTATTTGATGCCCATTTATAGATGTAGTGCGTTGCGGTTGTTGACGTAATGATTGCGtt        <  1:5513168/100‑1 (MQ=255)                                                                    gAAACGTTTTTGAATAATATTTTGAATGGTGTGTTGTATTTGATGCCCATTTATAGATGTAGTGCGTTGCGGTTGTTGACGTAATGATTGCGTTTGttct  <  1:3276575/100‑1 (MQ=255)                                                                                                |                                                                        TTCCTATAAGATTTTTATCTTTCGTAATGTGAATGGTTTCAATATAGGGGTACAATATGTTTAACGTGAAACGTTTTTGAATAATATTTTGAATGATGTGTTGTATTTGATGCCCATTGATAGATGTAGTGCGTTGCGGTTGTTGACGTAATGATTGTGTTTGCTCT  >  NC_007793/47604‑47770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑