Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,097,411	A→G	S1299S (AGT→AGC)	SAUSA300_RS10590 ←	phage tail tape measure protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,097,411	0	A	G	100.0%	10.5 / NA	6	S1299S (AGT→AGC)	SAUSA300_RS10590	phage tail tape measure protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (1/5);  total (1/5)

CTAAAAGTTGAGCCGATGATTTGGAATAATCCTCTTGATGGGTCTCCTCTTTGAGCATTAATATCCCAATTATTCACTGCATTTGATTGATAGTTACTTTCACGCTTTGCAACTCGCATCATCTGGTCATGAATCCACTTACCTTTATAACGTCCTCCTAAAATACTTTGCGCT  >  NC_007793/2097316‑2097489                                                                                                |                                                                               ctAAAAGTTGAGCCGATAATTTGGAATAATCCTCTAGACGGGTCTCCTCTTTGAGCATTAATATCCCAATTATTCACTGCATTTGATTGATAGTTGCttt                                                                            <  1:821139/100‑1 (MQ=255)                  aatTTGGAATAATCCTCTAGACGGGTCTCCTCTTTGAGCATTAATATCCCAATTATTCACTGCATTTGATTGATAGTTGCTTTCACGCTTTGCAACTCGc                                                           <  1:1397482/99‑1 (MQ=255)                              tCCTCTAGACGGGTCTCCTCTTTGAGCATTAATATCCCAATTATTCACTGCATTTGATTGATAGTTGCTTTCACGCTTTGCAACTCGCATCATCTGGTCa                                               <  1:3297791/100‑1 (MQ=255)                               ccTCTAGACGGGTCTCCTCTTTGAGCATTAATATCCCAATTATTCACTGCATTTGATTGATAGTTGCTTTCACGCTTTGCAACTCGCATCATCTGGTCAt                                              <  1:3028951/100‑1 (MQ=255)                                ctctAGACGGGTCTCCTCTTTGAGCATTAATATCCCAATTATTCACTGCATTTGATTGATAGTTGCTTTCACGCTTTGCAACTCGCATCATCTGGTCATg                                             <  1:4720701/100‑1 (MQ=255)                                                                           cACTGCATTTGATTGATAGTTGCTTTCACGCTTTGCAACTCGCATCATCTGGTCATGAATCCACTTACCTTTATAACGTCCTCCTAAAATACTTTGCGCt  >  1:341821/1‑100 (MQ=255)                                                                                                |                                                                               CTAAAAGTTGAGCCGATGATTTGGAATAATCCTCTTGATGGGTCTCCTCTTTGAGCATTAATATCCCAATTATTCACTGCATTTGATTGATAGTTACTTTCACGCTTTGCAACTCGCATCATCTGGTCATGAATCCACTTACCTTTATAACGTCCTCCTAAAATACTTTGCGCT  >  NC_007793/2097316‑2097489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑