Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	47,575	A→G	I439I (ATT→ATC)	SAUSA300_RS00205 ←	recombinase RecA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	47,575	0	A	G	100.0%	21.8 / NA	10	I439I (ATT→ATC)	SAUSA300_RS00205	recombinase RecA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/6);  total (4/6)

TACATAGATACCGACACGTTTTGTTTTAAGTTGTTGCATTATGTTACATCCTTTCTTCATTTATGCAATCGATGATTGCATGGTTTGATTGACAATATTGAGTGGTTCATTTTTGAAATAGATTCCTATAAGATTTTTATCTTTCGTAATGTGAATGGTTTCAATATAGGGGTACAA  >  NC_007793/47482‑47658                                                                                              |                                                                                    tACATAGATACCGACACGTTTTGTTTTAAGTTGTTGCATTATGTTACATCCTTTCTTCATTTATGCAATCGATGATTGCATGGCTTGATTGACGATATTg                                                                               <  1:1948957/100‑1 (MQ=255)  aCATAGATACCGACACGTTTTGTTTTAAGTTGTTGCATTATGTTACATCCTTTCTTCATTTATGCAATCGATGATTGCATGGCTTGATTGACGATATTGa                                                                              >  1:1535857/1‑100 (MQ=255)       gATACCGACACGTTTTGTTTTAAGTTGTTGCATTATGTTACATCCTTTCTTCATTTATGCAATCGATGATTGCATGGCTTGATTGACGATATTGAGTggg                                                                         <  1:3216682/100‑2 (MQ=255)         tACCGACACGTTTTGTTTTAAGTTGTTGCATTATGTTACATCCTTTCTTCATTTATGCAATCGATGATTGCATGGCTTGATTGACGATATTGAGTGGTTc                                                                       <  1:4013803/100‑1 (MQ=255)         cgatctcacGTTTTGTTTTAAGTTGTTGCATTATGTTACATCCTTTCTTCATTTATGCAATCGATGATTGCATGGCTTGATTGACGATATTGAGTGGTTc                                                                       <  1:1599462/94‑1 (MQ=255)                acGTTTTGTTTTAAGTTGTTGCATTATGTTACATCCTTTCTTCATTTATGCAATCGATGATTGCATGGCTTGATTGACGATATTGAGTGGTTCATTTTTg                                                                >  1:2143182/1‑100 (MQ=255)                              gttgttGCATTATGTTACATCCTTTCTTCATTTATGCAATCGATGATTGCATGGCTTGATTGACGATATTGAGTGGTTCATTTTTGAAATAGATTCCtat                                                  <  1:1941014/100‑1 (MQ=255)                                                                          gatTGCATGGCTTGATTGACGATATTGAGTGGTTCATTTTTGAAATAGATTCCTATAAGATTTTTATCTTTCGTAATGTGAATGGTTTCAATATAggggg      <  1:3696266/100‑2 (MQ=255)                                                                             tGCATGGCTTGATTGACGATATTGAGTGGTTCATTTTTGAAATAGATTCCTATAAGATTTTTATCTTTCGTAATGTGAATGGTTTCAATATAggggcaga   >  1:5104505/1‑96 (MQ=255)                                                                              gCATGGCTTGATTGACGATATTGAGTGGTTCATTTTTGAAATAGATTCCTATAAGATTTTTATCTTTCGTAATGTGAATGGTTTCAATATAGGGGTagat  >  1:4397140/1‑97 (MQ=255)                                                                                              |                                                                                    TACATAGATACCGACACGTTTTGTTTTAAGTTGTTGCATTATGTTACATCCTTTCTTCATTTATGCAATCGATGATTGCATGGTTTGATTGACAATATTGAGTGGTTCATTTTTGAAATAGATTCCTATAAGATTTTTATCTTTCGTAATGTGAATGGTTTCAATATAGGGGTACAA  >  NC_007793/47482‑47658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑