Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,097,351	T→A	S1319S (TCA→TCT)	SAUSA300_RS10590 ←	phage tail tape measure protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,097,351	0	T	A	100.0%	17.5 / NA	8	S1319S (TCA→TCT)	SAUSA300_RS10590	phage tail tape measure protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (2/6);  total (2/6)

ACCGGATTATTAAAGTTAGTATATCCACGTTTAGCGTTTGCTCTAAAAGTTGAGCCGATGATTTGGAATAATCCTCTTGATGGGTCTCCTCTTTGAGCATTAATATCCCAATTATTCACTGCATTTGATTGATAGTTACTTTCACGCTTTGCAACTCGCATCATCTGGTCATG  >  NC_007793/2097274‑2097446                                                                              |                                                                                                actggATTATTATAATTAGTGTACCCTCGTTTAGCGTTAGCTCTAAAAGTTGAGCCGATAATTTGGAATAATCCTCTAGACGGGTCTCCTCTTTGAGCAt                                                                           >  1:5458535/4‑100 (MQ=255)                     gtaccctcgcttAGCGTTAGCTCTAAAAGTTGAGCCGATAATTTGGAATAATCCTCTAGACGGGTCTCCTCTTTGAGCATTAATATCCCAATTATTCACt                                                       >  1:5472776/11‑100 (MQ=255)                                  gCGTTAGCTCTAAAAGTTGAGCCGATAATTTGGAATAATCCTCTAGACGGGTCTCCTCTTTGAGCATTAATATCCCAATTATTCACTGCATTtgattgat                                          <  1:3164081/100‑1 (MQ=255)                                           ctAAAAGTTGAGCCGATAATTTGGAATAATCCTCTAGACGGGTCTCCTCTTTGAGCATTAATATCCCAATTATTCACTGCATTTGATTGATAGTTGCttt                                 <  1:821139/100‑1 (MQ=255)                                                            aatTTGGAATAATCCTCTAGACGGGTCTCCTCTTTGAGCATTAATATCCCAATTATTCACTGCATTTGATTGATAGTTGCTTTCACGCTTTGCAACTCGc                <  1:1397482/99‑1 (MQ=255)                                                                        tCCTCTAGACGGGTCTCCTCTTTGAGCATTAATATCCCAATTATTCACTGCATTTGATTGATAGTTGCTTTCACGCTTTGCAACTCGCATCATCTGGTCa    <  1:3297791/100‑1 (MQ=255)                                                                         ccTCTAGACGGGTCTCCTCTTTGAGCATTAATATCCCAATTATTCACTGCATTTGATTGATAGTTGCTTTCACGCTTTGCAACTCGCATCATCTGGTCAt   <  1:3028951/100‑1 (MQ=255)                                                                          ctctAGACGGGTCTCCTCTTTGAGCATTAATATCCCAATTATTCACTGCATTTGATTGATAGTTGCTTTCACGCTTTGCAACTCGCATCATCTGGTCATg  <  1:4720701/100‑1 (MQ=255)                                                                              |                                                                                                ACCGGATTATTAAAGTTAGTATATCCACGTTTAGCGTTTGCTCTAAAAGTTGAGCCGATGATTTGGAATAATCCTCTTGATGGGTCTCCTCTTTGAGCATTAATATCCCAATTATTCACTGCATTTGATTGATAGTTACTTTCACGCTTTGCAACTCGCATCATCTGGTCATG  >  NC_007793/2097274‑2097446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑