Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,097,231	G→A	Y1359Y (TAC→TAT)	SAUSA300_RS10590 ←	phage tail tape measure protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,097,231	0	G	A	100.0%	21.6 / NA	9	Y1359Y (TAC→TAT)	SAUSA300_RS10590	phage tail tape measure protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (1/8);  total (1/8)

GGATGACCGTCTTCACCTAAGTTATACCAACCATCAAAAACTTTTCCACCTGTAGCATATGCGTAATCACCAGCACGCTTAAATCCATCCCAACCGTAGCGTCTAACAATGTACTGCATTGCTGAAATACCTTGATGAACCGGATTATTAAAGTTAGTATATCCACGTTTAGCGTT  >  NC_007793/2097136‑2097311                                                                                                |                                                                                 ggATGACCGTCTTCACCTAAGTTATACCAACCATCAAAAACTTTTCCACCTGTAGCATATGCGTAATCACCAGCACGTTTAAAACCACCCCAACCATAga                                                                              <  1:2725213/100‑2 (MQ=255)             cgatcTAAGTTATACCAACCATCAAAAACTTTTCCACCTGTAGCATATGCGTAATCACCAGCACGTTTAAAACCACCCCAACCATATCGTCTAACAATGt                                                                  <  1:4183968/96‑1 (MQ=255)                  tAAGTTATACCAACCATCAAAAACTTTTCCACCTGTAGCATATGCGTAATCACCAGCACGTTTAAAACCACCCCAACCATATCGTCTAACAATGTACTGc                                                             <  1:5338847/100‑1 (MQ=255)                                            ttCCACCTGTAGCATATGCGTAATCACCAGCACGTTTAAAACCACCCCATCCATATCGTCTAACAATGTACTGCATTGCTGAGATACCTTGATGTACTgg                                   <  1:5615444/100‑1 (MQ=255)                                            ctCCACCTGTAGCATATGCTTAATCACCAGCACGTTTAAAACCACCCCAACCATATCGTCTAACAATGTACTGCATTGCTGAGATACCTTGATGTACTgg                                   >  1:5172302/2‑100 (MQ=255)                                              cgatcTGTAGCATATGCGTAATCACCAGCACGTTTAAAACCACCCCAACCATATCGTCTAACAATGTACTGCATTGCTGAGATACCTTGATGTACTGGat                                 <  1:2842782/96‑1 (MQ=255)                                                             gCGTAATCACCAGCACGTTTAAAACCAACCCAACCATACCGTCTAACAATGTACTGCATTGCTGAGATACCTTGATGTACTGGATTATTATAATTAGtgt                  <  1:50486/100‑3 (MQ=255)                                                               gTAATCACCAGCACGTTTAAA‑CCACCCCAACCATATCGTCTAACAATGTACTGCATTGCTGAGATACCTTGATGTACTGGATTATTATAATTAGtgtacc               <  1:1148183/100‑3 (MQ=255)                                                                             gtttAAAACCACCCCAACCATATCGTCTAACAATGTACTGCATTGCTGAGATACCTTGATGTACTGGATTATTATAATTAGTGTACCCTCGTTTAGCGtt  <  1:4971068/98‑1 (MQ=255)                                                                                                |                                                                                 GGATGACCGTCTTCACCTAAGTTATACCAACCATCAAAAACTTTTCCACCTGTAGCATATGCGTAATCACCAGCACGCTTAAATCCATCCCAACCGTAGCGTCTAACAATGTACTGCATTGCTGAAATACCTTGATGAACCGGATTATTAAAGTTAGTATATCCACGTTTAGCGTT  >  NC_007793/2097136‑2097311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑