Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,096,046	A→G	S246P (TCT→CCT)	SAUSA300_RS10585 ←	phage tail protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,096,046	0	A	G	100.0%	36.2 / NA	15	S246P (TCT→CCT)	SAUSA300_RS10585	phage tail protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/11);  total (4/11)

GTTCAACAATACATTTATAGGTAATTTGAAAATCTGTCATCGCTTTAGGGAGCCCTCGTTTCGTGCCAGCACCAACCCAACCTTTTGCATCAGAAAAATTAGTTGCTTTATATCCTTCGCCAAGATTGGATATCACAAAGTCACCGCCGACTTTACCACCTAAATCATTACTTGG  >  NC_007793/2095953‑2096127                                                                                              |                                                                                  gTTCAACAATACATTTATAGGTAATTTGAAAATCTGTCATCGCTTTAGGGAGCCCTCGTTTCGTGCCAGCACCAACCCAACCTTTTGCATCAGGAAAAtt                                                                             >  1:1878927/1‑100 (MQ=255)                  taGGTAATTTGAAAATCTGTCATCGCTTTAGGGAGCCCTCGTTTCGTGCCAGCACCAACCCAACCTTTTGCATCAGGAAAATTAGTTGCTTTATATCCtt                                                            <  1:1720578/100‑1 (MQ=255)                      tAATTTGAAAATCTGTCATCGCTTTAGGGAGCCCTCGTTTCGTGCCAGCACCAACCCAACCTTTTGCATCAGGAAAATTAGTTGCTTTATATCCTTCGcc                                                        <  1:5433259/100‑1 (MQ=255)                       aaTTTGAAAATCTGTCATCGCTTTAGGGAGCCCTCGTTTCGTGCCAGCACCAACCCAACCTTTTGCATCAGGAAAATTAGTTGCTTTATATCCTTCGCCa                                                       <  1:1528738/100‑1 (MQ=255)                       aaTTTGAAAATCTGTCATCGCTTTAGGGAGCCCTCGTTTCGTGCCAGCACCAACCCAACCTTTTGCATCAGGAAAATTAGTTGCTTTATATCCTTCGCCa                                                       <  1:2957459/100‑1 (MQ=255)                       aaTTTGAAAATCTGTCATCGCTTTAGGGAGCCCTCGTTTCGTGCCAGCACCAACCCAACCTTTTGCATCAGGAAAATTAGTTGCTTTATATCCTTCGCCa                                                       <  1:3223471/100‑1 (MQ=255)                       aaTTTGAAAATCTGTCATCGCTTTAGGGAGCCCTCGTTTCGTGCCAGCACCAACCCAACCTTTTGCATCAGGAAAATTAGTTGCTTTATATCCTTCGCCa                                                       <  1:618732/100‑1 (MQ=255)                       aaTTTGAAAATCTGTCATCGCTTTAGGGAGCCCACGTTTCGTGCCAGCACCAACCCAACCTTTTGCATCAGGAAAATTAGTTGCTTTATATCCTTCGCCa                                                       <  1:1140659/100‑1 (MQ=255)                                  gtGTCATCGCTTTAGGGAGCCCTCGTGTCGTGCCAGCACCAACCCAACCTTTTGCATCAGGAAAATTAGTTGCTTTATATCCTGCGCCAAGATCGGaaga                                            >  1:5606929/2‑97 (MQ=255)                                                          gTTTCGTGCCAGCACCAACCCAACCTTTTGCATCAGGAAAATTAGTTGCTTTATATCCTTCGCCAAGATTGGATATCACAAAGTCACCGCCGACCTTacc                    <  1:4052772/100‑1 (MQ=255)                                                              cGTGCCAGCACCAACCCAACCTTTTGCATCAGGAAAATTAGTTGCTTTATATCCTTCGCCAAGATTGGATATCACAAAGTCACCGCCGACCTTaccacag                <  1:2390512/100‑3 (MQ=255)                                                              cGTGCCAGCACCAACCCAACCTTTTGCATCAGGAAAATTAGTTGCTTTATATCCTTCGCCAAGATTGGATATCACAAAGTCACCGCCGACCTTACCACCt                <  1:1196222/100‑1 (MQ=255)                                                                tGCCAGCACCAACCCAACCTTTTGCATCAGGAAAATTAGTTGCTTTATATCCCTCGCCAAGATTGGATATCACAAAGTCACCGCCGACCTTACCACCTaa              >  1:2763590/1‑100 (MQ=255)                                                                   cAGCACCAACCCAACCTTTTGCATCAGGAAAATTAGTTGCTTTATATCCTTCGCCAAGATTGGATATCACAAAGTCACCGCCGACCTTACCACCTAAGTc           >  1:4181308/1‑100 (MQ=255)                                                                            cccaaccTTTTGCATCAGGAAAATTAGTTGCTTTATATCCTTCGCCAAGATTGGATATCACAAAGTCACCGCCGACCTTACCACCTAAGTCATTACTTgg  <  1:3950930/100‑1 (MQ=255)                                                                                              |                                                                                  GTTCAACAATACATTTATAGGTAATTTGAAAATCTGTCATCGCTTTAGGGAGCCCTCGTTTCGTGCCAGCACCAACCCAACCTTTTGCATCAGAAAAATTAGTTGCTTTATATCCTTCGCCAAGATTGGATATCACAAAGTCACCGCCGACTTTACCACCTAAATCATTACTTGG  >  NC_007793/2095953‑2096127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑