Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,094,805	C→T	L159L (TTG→TTA)	SAUSA300_RS10580 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,094,805	0	C	T	100.0%	30.3 / NA	13	L159L (TTG→TTA)	SAUSA300_RS10580	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/7);  total (6/7)

GAATAAGCTGTTTTTCTTTTTGAGTACCACATATCTACCTTTGACGGTATTAGAACTAAGCTCTATATAAAAATCCAATACCATTTTATAGGTTGTACATAATTGCTTTAAAACTTCATATCTAGTTTGATAAGAAGTCCATGACGTAGTACGTAAGCCATCGTATTCG  >  NC_007793/2094730‑2094898                                                                            |                                                                                              gAATAAGCTGTTTTTCTTTTTGAGTACTACATATCTACCTTTGACGGTATTAGAGCTAAGCTCAATATAAAAATCTAAAACCATTTTATAGGTTGTACAt                                                                       <  1:137892/100‑1 (MQ=255)   aTAAGCTGTTTTTCTTTTTGAGTACTACATATCTACCTTTGACGGTATTAGAGCTAAGCTCAATATAAAAATCTAAAACCATTTTATAGGTTGTACagca                                                                     <  1:4221504/100‑4 (MQ=255)        cTGTTTTTCTTTTTGAGTACTACATATCTACCTTTGACGGTATTAGAGCTAAGCTCAATATAAAAATCTAAAACCATTTTATAGGTTGTACATAATTGCt                                                                <  1:890169/100‑1 (MQ=255)                            tacaTATCTACCTTTGACGGTATTAGAGCTAAGCTCAATATAAAAATCTAAAACCATTTTATAGGTTGTACATAATTGCTTTAAAACTTCATATCTAGtt                                            >  1:2850016/2‑100 (MQ=255)                             acaTATCTACCTTTGACGGTATTAGAGCTAAGCTCAATATAAAAATCTAAAACCATTTTATAGGTTGTACATAATTGCTTTAAAACTTCATATCTAGttt                                           >  1:3863110/1‑100 (MQ=255)                                tatCTACCTTTGACGGTATTAGAGCTAAGCTCAATATAAAAATCTAAAACCATTTTATAGGTTGTACATAATTGCTTTAAAACTTCATATCTAGTTTGAt                                        <  1:3092827/100‑1 (MQ=255)                                 atCTACCTTTGACGGTATTAGAGCTAAGCTCAATATAAAAATCTAAAACCATTTTATAGGTTGTACATAATTGCTTTAAAACTTCATATCTAGTTTGATa                                       >  1:820173/1‑100 (MQ=255)                                     aCCTTTGACGGTATTAGAGCTAAGCTCAATATAAAAATCTAAAACCATTTTATAGGTTGTACATAATTGCTTTAAAACTTCATATCTAGTTTGATaagaa                                   >  1:1834162/1‑100 (MQ=255)                                     aCCTTTGACGGTATTAGAGCTAAGCTCAATATAAAAATCTAAAACCATTTTATAGGTTGTACATAATTGCTTTAAAACTTCATATCTAGTTTGATaagaa                                   >  1:1888988/1‑100 (MQ=255)                                     aCCTTTGACGGTATTAGAGCTAAGCTCAATATAAAAATCTAAAACCATTTTATAGGTTGTACATAATTGCTTTAAAACTTCATATCTAGTTTGATaagaa                                   >  1:582749/1‑100 (MQ=255)                                                   tAGAGCTAAGCTCAATATAAAAATCTAAAACCATTTTATAGGTTGTACATAATTGCTTTAAAACTTCATATCTAGTTTGATAAGAAGTCCATGACgtagt                     <  1:398918/100‑1 (MQ=255)                                                               caatataAAAATCTAAAACCATTTTATAGGTTGTACATAATTGCTTTAAAACTTCATATCTAGTTTGATAAGAAGTCCATGACGTAGTACGTAAGCCAtg         <  1:5410517/98‑2 (MQ=255)                                                                      aaaaTCTAAAACCATTTTATAGGTTGTACATAATTGCTTTAAAACTTCATATCTAGTTTGATAAGAAGTCCATGACGTAGTACGTAAGCCATCGTATTca  <  1:4099109/100‑2 (MQ=255)                                                                            |                                                                                              GAATAAGCTGTTTTTCTTTTTGAGTACCACATATCTACCTTTGACGGTATTAGAACTAAGCTCTATATAAAAATCCAATACCATTTTATAGGTTGTACATAATTGCTTTAAAACTTCATATCTAGTTTGATAAGAAGTCCATGACGTAGTACGTAAGCCATCGTATTCG  >  NC_007793/2094730‑2094898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑