Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,094,793	T→A	I163I (ATA→ATT)	SAUSA300_RS10580 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,094,793	0	T	A	100.0%	29.9 / NA	12	I163I (ATA→ATT)	SAUSA300_RS10580	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (6/6);  total (6/6)

ACCATACTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACCACATATCTACCTTTGACGGTATTAGAACTAAGCTCTATATAAAAATCCAATACCATTTTATAGGTTGTACATAATTGCTTTAAAACTTCATATCTAGTTTGATAAGAAGTCCATGACGTAGT  >  NC_007793/2094706‑2094879                                                                                        |                                                                                       aCCATATTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACTACATATCTACCTTTGACGGTATTAGAGCTAAGCTCAATATAAAAATct                                                                            <  1:4031893/100‑2 (MQ=255)                         gAATAAGCTGTTTTTCTTTTTGAGTACTACATATCTACCTTTGACGGTATTAGAGCTAAGCTCAATATAAAAATCTAAAACCATTTTATAGGTTGTACAt                                                    <  1:137892/100‑1 (MQ=255)                           aTAAGCTGTTTTTCTTTTTGAGTACTACATATCTACCTTTGACGGTATTAGAGCTAAGCTCAATATAAAAATCTAAAACCATTTTATAGGTTGTACagca                                                  <  1:4221504/100‑4 (MQ=255)                                cTGTTTTTCTTTTTGAGTACTACATATCTACCTTTGACGGTATTAGAGCTAAGCTCAATATAAAAATCTAAAACCATTTTATAGGTTGTACATAATTGCt                                             <  1:890169/100‑1 (MQ=255)                                                    tacaTATCTACCTTTGACGGTATTAGAGCTAAGCTCAATATAAAAATCTAAAACCATTTTATAGGTTGTACATAATTGCTTTAAAACTTCATATCTAGtt                         >  1:2850016/2‑100 (MQ=255)                                                     acaTATCTACCTTTGACGGTATTAGAGCTAAGCTCAATATAAAAATCTAAAACCATTTTATAGGTTGTACATAATTGCTTTAAAACTTCATATCTAGttt                        >  1:3863110/1‑100 (MQ=255)                                                        tatCTACCTTTGACGGTATTAGAGCTAAGCTCAATATAAAAATCTAAAACCATTTTATAGGTTGTACATAATTGCTTTAAAACTTCATATCTAGTTTGAt                     <  1:3092827/100‑1 (MQ=255)                                                         atCTACCTTTGACGGTATTAGAGCTAAGCTCAATATAAAAATCTAAAACCATTTTATAGGTTGTACATAATTGCTTTAAAACTTCATATCTAGTTTGATa                    >  1:820173/1‑100 (MQ=255)                                                             aCCTTTGACGGTATTAGAGCTAAGCTCAATATAAAAATCTAAAACCATTTTATAGGTTGTACATAATTGCTTTAAAACTTCATATCTAGTTTGATaagaa                >  1:1834162/1‑100 (MQ=255)                                                             aCCTTTGACGGTATTAGAGCTAAGCTCAATATAAAAATCTAAAACCATTTTATAGGTTGTACATAATTGCTTTAAAACTTCATATCTAGTTTGATaagaa                >  1:1888988/1‑100 (MQ=255)                                                             aCCTTTGACGGTATTAGAGCTAAGCTCAATATAAAAATCTAAAACCATTTTATAGGTTGTACATAATTGCTTTAAAACTTCATATCTAGTTTGATaagaa                >  1:582749/1‑100 (MQ=255)                                                                           tAGAGCTAAGCTCAATATAAAAATCTAAAACCATTTTATAGGTTGTACATAATTGCTTTAAAACTTCATATCTAGTTTGATAAGAAGTCCATGACgtagt  <  1:398918/100‑1 (MQ=255)                                                                                        |                                                                                       ACCATACTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACCACATATCTACCTTTGACGGTATTAGAACTAAGCTCTATATAAAAATCCAATACCATTTTATAGGTTGTACATAATTGCTTTAAAACTTCATATCTAGTTTGATAAGAAGTCCATGACGTAGT  >  NC_007793/2094706‑2094879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑