Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,094,757	C→T	V175V (GTG→GTA)	SAUSA300_RS10580 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,094,757	0	C	T	100.0%	41.3 / NA	16	V175V (GTG→GTA)	SAUSA300_RS10580	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/7);  total (9/7)

GACATATCAATCTTCCTAGTTAACCCAACCAAATCTTTACCATACTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACCACATATCTACCTTTGACGGTATTAGAACTAAGCTCTATATAAAAATCCAATACCATTTTATAGGTTGTACATAATTGCT  >  NC_007793/2094668‑2094836                                                                                          |                                                                                gACATATCAATCTTCCTAGTTAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACTACATATCTAc                                                                       >  1:2474919/1‑100 (MQ=255) gACATATCAATCTTCCTAGTTAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACTACATATCTAc                                                                       >  1:3772073/1‑100 (MQ=255) gACATATCAATCTTCCTAGTTAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACTACATATCTAc                                                                       >  1:4038181/1‑100 (MQ=255) gACATATCAATCTTCCTAGTTAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACTACATATATAc                                                                       >  1:3394741/1‑100 (MQ=255)      tgtgctCTTCCGATCTAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACTACATATCTACCttat                                                                  <  1:5275690/95‑3 (MQ=255)           tCTTCCGATCTAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACTACATATCTACCTTTGACggc                                                             <  1:4979499/100‑2 (MQ=255)            tttCCTAGTTAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACTACATATCTACCTTTGACGGTa                                                            >  1:2856852/2‑100 (MQ=255)              tCCTAGTTAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACTACATATCTACCtttagatcggaa                                                          >  1:523530/1‑91 (MQ=255)              tCCTAGTTAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACTACATATCTACCTTAGatcggaag                                                          >  1:3113749/1‑93 (MQ=255)               ccTAGTTAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACTACATATCTACCTTTGACGGTATTa                                                         >  1:2901701/1‑100 (MQ=255)                     tAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACTACATATCTACCTTTGACGGTATTAGAGCTa                                                   <  1:450721/100‑1 (MQ=255)                     tAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACTACATATCTACCTTTGACGGTATTAGAGCTa                                                   >  1:4666841/1‑100 (MQ=255)                                       aCCATATTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACTACATATCTACCTTTGACGGTATTAGAGCTAAGCTCAATATAAAAATct                                 <  1:4031893/100‑2 (MQ=255)                                                               gAATAAGCTGTTTTTCTTTTTGAGTACTACATATCTACCTTTGACGGTATTAGAGCTAAGCTCAATATAAAAATCTAAAACCATTTTATAGGTTGTACAt         <  1:137892/100‑1 (MQ=255)                                                                 aTAAGCTGTTTTTCTTTTTGAGTACTACATATCTACCTTTGACGGTATTAGAGCTAAGCTCAATATAAAAATCTAAAACCATTTTATAGGTTGTACagca       <  1:4221504/100‑4 (MQ=255)                                                                      cTGTTTTTCTTTTTGAGTACTACATATCTACCTTTGACGGTATTAGAGCTAAGCTCAATATAAAAATCTAAAACCATTTTATAGGTTGTACATAATTGCt  <  1:890169/100‑1 (MQ=255)                                                                                          |                                                                                GACATATCAATCTTCCTAGTTAACCCAACCAAATCTTTACCATACTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACCACATATCTACCTTTGACGGTATTAGAACTAAGCTCTATATAAAAATCCAATACCATTTTATAGGTTGTACATAATTGCT  >  NC_007793/2094668‑2094836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑