Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,094,712	C→T	E190E (GAG→GAA)	SAUSA300_RS10580 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,094,712	0	C	T	100.0%	49.7 / NA	19	E190E (GAG→GAA)	SAUSA300_RS10580	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/6);  total (13/6)

TCCTTTGTCATTTTCGGGTCCCACAGCAATTAATGCTGTTTTGATTTCTGACATATCAATCTTCCTAGTTAACCCAACCAAATCTTTACCATACTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACCACATATCTACCTTTGACGGTATTAGAACTAAGCTCTATATAAAAATCC  >  NC_007793/2094619‑2094805                                                                                              |                                                                                              ccctacgTCATTTTCAGGTCCCACAGCAATTAATGCTGTTTTGATTTCTGACATATCAATCTTCCTAGTTAACCCGACTAAATCTTTACCATATTCAAtt                                                                                         >  1:2573124/7‑100 (MQ=255) cccTTTGTCATTTTCAGGTCCCACAGCAATTAATGCTGTTTTGATTTCTGACATATCAATCTTCCTAGTTAACCCGACTAAATCTTTACCATATTCAAtt                                                                                         >  1:3159980/2‑100 (MQ=255) cccTTTGTCATTTTCAGGTCCCACAGCAATTAATGCTGTTTTGATTTCTGACATATCAATCTTCCTAGTTAACCCGACTAAATCTTTACCATATTCAAtt                                                                                         >  1:322442/2‑100 (MQ=255) cccTTTGTCATTTTCAGGTCCCACAGCAATTAATGCTGTTTTGATTTCTGACATATCAATCTTCCTAGTTAACCCGACTAAATCTTTACCATATTCAAtt                                                                                         >  1:3566925/2‑100 (MQ=255)           ttttCAGGTCCCACAGCAATTAATGCTGTTTTGATTTCTGACATATCAATCTTCCTAGTTAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCtt                                                                               <  1:2438474/100‑1 (MQ=255)                         aGCAATTAATGCTGTTTTGATTTCTGACATATCAATCTTCCTAGTTAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCTTTGAATAAGCTGttt                                                                 <  1:4985657/100‑1 (MQ=255)                                                  gACATATCAATCTTCCTAGTTAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACTACATATCTAc                                        >  1:2474919/1‑100 (MQ=255)                                                  gACATATCAATCTTCCTAGTTAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACTACATATCTAc                                        >  1:4038181/1‑100 (MQ=255)                                                  gACATATCAATCTTCCTAGTTAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACTACATATCTAc                                        >  1:3772073/1‑100 (MQ=255)                                                  gACATATCAATCTTCCTAGTTAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACTACATATATAc                                        >  1:3394741/1‑100 (MQ=255)                                                       tgtgctCTTCCGATCTAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACTACATATCTACCttat                                   <  1:5275690/95‑3 (MQ=255)                                                            tCTTCCGATCTAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACTACATATCTACCTTTGACggc                              <  1:4979499/100‑2 (MQ=255)                                                             tttCCTAGTTAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACTACATATCTACCTTTGACGGTa                             >  1:2856852/2‑100 (MQ=255)                                                               tCCTAGTTAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACTACATATCTACCtttagatcggaa                           >  1:523530/1‑91 (MQ=255)                                                               tCCTAGTTAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACTACATATCTACCTTAGatcggaag                           >  1:3113749/1‑93 (MQ=255)                                                                ccTAGTTAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACTACATATCTACCTTTGACGGTATTa                          >  1:2901701/1‑100 (MQ=255)                                                                      tAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACTACATATCTACCTTTGACGGTATTAGAGCTa                    <  1:450721/100‑1 (MQ=255)                                                                      tAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACTACATATCTACCTTTGACGGTATTAGAGCTa                    >  1:4666841/1‑100 (MQ=255)                                                                                        aCCATATTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACTACATATCTACCTTTGACGGTATTAGAGCTAAGCTCAATATAAAAATct  <  1:4031893/100‑2 (MQ=255)                                                                                              |                                                                                              TCCTTTGTCATTTTCGGGTCCCACAGCAATTAATGCTGTTTTGATTTCTGACATATCAATCTTCCTAGTTAACCCAACCAAATCTTTACCATACTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACCACATATCTACCTTTGACGGTATTAGAACTAAGCTCTATATAAAAATCC  >  NC_007793/2094619‑2094805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑