Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,094,694	A→G	V196V (GTT→GTC)	SAUSA300_RS10580 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,094,694	0	A	G	100.0%	52.1 / NA	21	V196V (GTT→GTC)	SAUSA300_RS10580	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/7);  total (14/7)

AAACGCTTTCCTTTGTCATTTTCGGGTCCCACAGCAATTAATGCTGTTTTGATTTCTGACATATCAATCTTCCTAGTTAACCCAACCAAATCTTTACCATACTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACCACATATCTACCTTTGACGGTATTAGAACTA  >  NC_007793/2094611‑2094787                                                                                    |                                                                                              aaaCGCTTCCCTTTGTCATTTTCAGGTCCCACAGCAATTAATGCTGTTTTGATTTCTGACATATCAATCTTCCTAGTTAACCCGACTAAATCTTTACCAt                                                                               <  1:4182152/100‑1 (MQ=255) aaaCGCTTCCCTTTGTCATTTTCAGGTCCCACAGCAATTAATGCTGTTTTGATTTCTGACATATCAATCTTCCTAGTTAACCCGACTAAATCTTTACCAt                                                                               >  1:3390532/1‑100 (MQ=255) aaaCGCTTCCCTTTGTCATTTTCAGATCCCACAGCAATTAATGCTGTTTTGATTTCTGACATATCAATCTTCCTAGTTAACCCGACTAAATCTTTACCAt                                                                               <  1:4751712/100‑1 (MQ=255)         ccctacgTCATTTTCAGGTCCCACAGCAATTAATGCTGTTTTGATTTCTGACATATCAATCTTCCTAGTTAACCCGACTAAATCTTTACCATATTCAAtt                                                                       >  1:2573124/7‑100 (MQ=255)         cccTTTGTCATTTTCAGGTCCCACAGCAATTAATGCTGTTTTGATTTCTGACATATCAATCTTCCTAGTTAACCCGACTAAATCTTTACCATATTCAAtt                                                                       >  1:3159980/2‑100 (MQ=255)         cccTTTGTCATTTTCAGGTCCCACAGCAATTAATGCTGTTTTGATTTCTGACATATCAATCTTCCTAGTTAACCCGACTAAATCTTTACCATATTCAAtt                                                                       >  1:322442/2‑100 (MQ=255)         cccTTTGTCATTTTCAGGTCCCACAGCAATTAATGCTGTTTTGATTTCTGACATATCAATCTTCCTAGTTAACCCGACTAAATCTTTACCATATTCAAtt                                                                       >  1:3566925/2‑100 (MQ=255)                   ttttCAGGTCCCACAGCAATTAATGCTGTTTTGATTTCTGACATATCAATCTTCCTAGTTAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCtt                                                             <  1:2438474/100‑1 (MQ=255)                                 aGCAATTAATGCTGTTTTGATTTCTGACATATCAATCTTCCTAGTTAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCTTTGAATAAGCTGttt                                               <  1:4985657/100‑1 (MQ=255)                                                          gACATATCAATCTTCCTAGTTAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACTACATATCTAc                      >  1:2474919/1‑100 (MQ=255)                                                          gACATATCAATCTTCCTAGTTAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACTACATATCTAc                      >  1:4038181/1‑100 (MQ=255)                                                          gACATATCAATCTTCCTAGTTAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACTACATATCTAc                      >  1:3772073/1‑100 (MQ=255)                                                          gACATATCAATCTTCCTAGTTAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACTACATATATAc                      >  1:3394741/1‑100 (MQ=255)                                                               tgtgctCTTCCGATCTAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACTACATATCTACCttat                 <  1:5275690/95‑3 (MQ=255)                                                                    tCTTCCGATCTAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACTACATATCTACCTTTGACggc            <  1:4979499/100‑2 (MQ=255)                                                                     tttCCTAGTTAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACTACATATCTACCTTTGACGGTa           >  1:2856852/2‑100 (MQ=255)                                                                       tCCTAGTTAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACTACATATCTACCtttagatcggaa         >  1:523530/1‑91 (MQ=255)                                                                       tCCTAGTTAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACTACATATCTACCTTAGatcggaag         >  1:3113749/1‑93 (MQ=255)                                                                        ccTAGTTAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACTACATATCTACCTTTGACGGTATTa        >  1:2901701/1‑100 (MQ=255)                                                                              tAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACTACATATCTACCTTTGACGGTATTAGAGCTa  <  1:450721/100‑1 (MQ=255)                                                                              tAACCCGACTAAATCTTTACCATATTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACTACATATCTACCTTTGACGGTATTAGAGCTa  >  1:4666841/1‑100 (MQ=255)                                                                                    |                                                                                              AAACGCTTTCCTTTGTCATTTTCGGGTCCCACAGCAATTAATGCTGTTTTGATTTCTGACATATCAATCTTCCTAGTTAACCCAACCAAATCTTTACCATACTCAATTTCTTTACCTTTGAATAAGCTGTTTTTCTTTTTGAGTACCACATATCTACCTTTGACGGTATTAGAACTA  >  NC_007793/2094611‑2094787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑