Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,094,619	T→C	G221G (GGA→GGG)	SAUSA300_RS10580 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,094,619	0	T	C	100.0%	18.6 / NA	9	G221G (GGA→GGG)	SAUSA300_RS10580	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/5);  total (4/5)

GATTGTGGTTCATATATCCCCCAAATATAGCGCATAGGTAGGTTGAATTGACTTTGCGCTTCGTCATCTGTCACAACTAGCTCTAAACGCTTTCCTTTGTCATTTTCGGGTCCCACAGCAATTAATGCTGTTTTGATTTCTGACATATCAATCTTCCTAGTTAACCCAACCAAATCTTTACCAT  >  NC_007793/2094527‑2094710                                                                                             |                                                                                            gATTGTGGTTCATATATCCCCCAAATATAGCGCATAGGTAGGTTGAATTGACTTTGCGCTTAGTCATCTGTCACAACTAGCTCTAAACGCTTCCCTTTGt                                                                                      >  1:4779016/1‑100 (MQ=255)               tatCCCCCAAATATAGCGCATAGGTAGGTTGAATTGACTTTGCGCTTCGTCATCTGTCACAACTAGCTCTAAACGCTTCCCTTTGTCATTTTCAGGTccc                                                                        <  1:5358958/100‑1 (MQ=255)                     ccAAATATAGCGCATAGGTAGGTTGAATTGACTTTGCGCTTCGTCATCTGTCACAACTAGCTCTAAACGCTTCCCTTTGTCATTTTCAGGTCCCACAGCa                                                                  <  1:3144824/100‑1 (MQ=255)                     ccAAATATAGCGCATAGGTAGGTTGAATTGACTTTGCGCTTCGTCATCTGTCACAACTAGCTCTAAACGCTTCCCTTTGTCATTTTCAGGTCCCACAGCa                                                                  >  1:5344354/1‑100 (MQ=255)                          tcaggtgcATAGGTAGGTTGAATTGACTTTGCGCTTCGTCATCTGTCACAACTAGCTCTAAACGCTTCCCTTTGTCATTTTCAGGTCCCACAGCAATTaa                                                             >  1:2473199/7‑100 (MQ=255)                                                              cgatctCTGTCACAACTAGCTCTAAACGCTTCCCTTTGTCATTTTCAGGTCCCACAGCAATTAATGCTGTTTTGATTTCTGACATATCAATCTTCCTAGt                         <  1:5592390/95‑1 (MQ=255)                                                                                     aaaCGCTTCCCTTTGTCATTTTCAGGTCCCACAGCAATTAATGCTGTTTTGATTTCTGACATATCAATCTTCCTAGTTAACCCGACTAAATCTTTACCAt  >  1:3390532/1‑100 (MQ=255)                                                                                     aaaCGCTTCCCTTTGTCATTTTCAGGTCCCACAGCAATTAATGCTGTTTTGATTTCTGACATATCAATCTTCCTAGTTAACCCGACTAAATCTTTACCAt  <  1:4182152/100‑1 (MQ=255)                                                                                     aaaCGCTTCCCTTTGTCATTTTCAGATCCCACAGCAATTAATGCTGTTTTGATTTCTGACATATCAATCTTCCTAGTTAACCCGACTAAATCTTTACCAt  <  1:4751712/100‑1 (MQ=255)                                                                                             |                                                                                            GATTGTGGTTCATATATCCCCCAAATATAGCGCATAGGTAGGTTGAATTGACTTTGCGCTTCGTCATCTGTCACAACTAGCTCTAAACGCTTTCCTTTGTCATTTTCGGGTCCCACAGCAATTAATGCTGTTTTGATTTCTGACATATCAATCTTCCTAGTTAACCCAACCAAATCTTTACCAT  >  NC_007793/2094527‑2094710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑