Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,090,879	A→G	N28N (AAT→AAC)	SAUSA300_RS10565 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,090,879	0	A	G	100.0%	30.6 / NA	13	N28N (AAT→AAC)	SAUSA300_RS10565	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/5);  total (8/5)

TTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACAC  >  NC_007793/2090801‑2090972                                                                               |                                                                                              ttctttTTGAATAGCATCTAAGGTTTTACCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCGTTGAGTGTGCTGAGCATTGtt                                                                          >  1:4288305/1‑100 (MQ=255)             cacATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCGTTGAGTGTGCTGAGCATTGTTTTATCATTctct                                                              >  1:305845/3‑100 (MQ=255)                                aaTTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCGTTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGcc                                           >  1:2026220/1‑100 (MQ=255)                                              ttGCTCTTGGGTTTTTTGACCTAATTTAATTTCGTTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCgtgt                             <  1:1940942/100‑1 (MQ=255)                                               tGCTCTTGGGTTTTTTGACCTAATTTAATTTCGTTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCCTTCATGTTCGTGtt                            >  1:5755899/1‑100 (MQ=255)                                               tGCTCTTGGGTTTTTTGACCTAATTTAATTTCGTTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGtt                            >  1:2921550/1‑100 (MQ=255)                                               tGCTCTTGGGTTTTTTGACCTAATTTAATTTCGTTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGtt                            >  1:3470191/1‑100 (MQ=255)                                                     tGGGTTTTTTGACCTAATTTAATTTCGTTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGa                      >  1:1921866/1‑100 (MQ=255)                                                               gACCTAATTTAATTTCGTTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCag            >  1:5324739/1‑99 (MQ=255)                                                                  cTAATTTAATTTCGTTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTc         <  1:146043/100‑1 (MQ=255)                                                                     atttaatttCGTTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGt      <  1:1781326/100‑1 (MQ=255)                                                                     atttaatttCGTTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGt      <  1:4647842/100‑1 (MQ=255)                                                                         aatttCGTTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTacag  <  1:1734815/100‑2 (MQ=255)                                                                               |                                                                                              TTCTTTTTGAATAGCATCTAAGGTTTTATCTAATTTAATGTTAACTTGCTCTTGGGTTTTTTGACCTAATTTAATTTCATTGAGTGTGCTGAGCATTGTTTTATCATTCTCTTCTAACCTTCTGATGCGCCATTCATGTTCGTGTTTTTTGAACCACCCCAATTCAGTACAC  >  NC_007793/2090801‑2090972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑