Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,063,322	A→G	Y238H (TAT→CAT)	SAUSA300_RS10385 ←	sodium‑dependent dicarboxylate transporter SdcS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,063,322	0	A	G	100.0%	86.6 / NA	34	Y238H (TAT→CAT)	SAUSA300_RS10385	sodium‑dependent dicarboxylate transporter SdcS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/29);  total (5/29)

TAAACGCAACATATCTTAAATAGAGCCAAGTAATACCTAACAAAACAATGACCGTTGGAATCCCTACAATCATCCATTTAGCAAAACTAATTTCATATCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGTTCCGATTAATGTACCCAAGCCACCAATCGTACCTGCATAGCCAAT  >  NC_007793/2063226‑2063415                                                                                                 |                                                                                              tAAACGCAACATATCTTAAATAGAGCCAAGTAATACCTAACAAAACAATGACCGTTGGAATCCCTACAATCATCCATTTAGCAAATCTAATTTCATGTcc                                                                                            <  1:5659805/100‑1 (MQ=255)   aaCGCAACATATCTTAAATAGAGCCAAGTAATACCTAACAAAACAATGACCGTTGGAATCCCTACAATCATCCATTTAGCAAAACTAATTTCATGTCCag                                                                                          <  1:449427/100‑2 (MQ=255)           atatCTTAAATAGAGCCAAGTAATACCTAACAAAACAATGACCGTTGGAATCCCTACAATCATCCATTTAGCAAAACTAATTTCATGTCCAAAATGTTGc                                                                                  <  1:511599/100‑1 (MQ=255)                 tAAATAGAGCCAAGTAATACCTAACAAAACAATGACCGTTGGAATCCCTACAATCATCCATTTAGCAAAACTAATTTCATGTCCAAAATGTTGCATGTAt                                                                            >  1:5778218/1‑100 (MQ=255)                   aaTAGAGCCAAGTAATACCTAACAAAACAATGACCGTTGGAATCCCTACAATCATCCATTTAGCAAAACTAATTTCATGTCCAAAATGTTGCATGTATTg                                                                          <  1:2343607/100‑1 (MQ=255)                      agagCCAAGTAATACCTAACAAAACAATGACCGTTGGAATCCCTACAATCATCCA‑TTAGCAAAACTAATTTCATGTCCAAAATGTTGCATGTATTGTccc                                                                      <  1:127554/100‑2 (MQ=255)                                    ccTAACAAAACAATGACCGTTGGAATCCCTACAATCATCCATTTAGCAAAACTAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAAtaatta                                                         <  1:5177907/100‑1 (MQ=255)                                    ccTAACAAAACAATGACCGTTGGAATCCCTACAATCATCCATTTAGCAAAACTAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAAtaatta                                                         <  1:1166221/100‑1 (MQ=255)                                           aaacaaTGACCGTTGGAATCCCTACAATCATCCATTTAGCAAAACTAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGcaa                                                  <  1:3400835/100‑3 (MQ=255)                                           aaacaaTGACCGTTGGAATCCCTACAATCATCCATTTAGCAAAACTAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCgg                                                  <  1:1951451/100‑1 (MQ=255)                                           aaacaaTGACCGTTGGAATCCCTACAATCATCCATTTAGCAAAACTAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCgg                                                  <  1:3079233/100‑1 (MQ=255)                                             ccaaTGACCGTTGGAATCCCTACAATCATCCATTTAGCAAAACTAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCgggg                                                >  1:1425066/2‑100 (MQ=255)                                                       ttGGAATCCCTACAATCATCCATTTAGCAAAACTAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGGTCCGATTaa                                      >  1:4983771/1‑100 (MQ=255)                                                           aaTCCCTACAATCATCCATTTAGCAAAACTAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGTTcccctttattta                                  >  1:2549739/1‑90 (MQ=255)                                                                  aCAATCATCCATTTAGCAAAACTAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGGTCCGATTTATTTAccccaac                           >  1:1024273/1‑96 (MQ=255)                                                                               tAGCAAAACTAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGTTCCGATTAATGTACCCAAGCCACCAATccacac              <  1:5552262/100‑6 (MQ=255)                                                                                 gCAAAACTAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGTTCCGATTAATGTACCCAAGCCACCAATCGTACCTg            <  1:354132/100‑1 (MQ=255)                                                                                  cAAAACTAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGTTCCGATTAATGTACCCAAGCCACCAATCGTACCTGc           <  1:2297236/100‑1 (MQ=255)                                                                                  cAAAACTAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGTTCCGATTAATGTACCCAAGCCACCAATCGTACCTGc           <  1:5155464/100‑1 (MQ=255)                                                                                  cAAAACTAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGTTCCGATTAATGTACCCAAGCCACCAATCGTACCTGc           <  1:3209132/100‑1 (MQ=255)                                                                                  cAAAACTAATTTCATGTCCAAAATGTTGCATGTATTGTCCTGTTAAAATAATTAATGGCGGGGTTGCGATTAATGTACCCAAGCCACCAATCGTACCTGc           <  1:749521/100‑1 (MQ=255)                                                                                    aaaCTAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGTTCCGATTAATGTACCCAAGCCACCAATCGTACCTGCAt         <  1:4410230/100‑1 (MQ=255)                                                                                    aaaCTAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGTTCCGATTAATGTACCCAAGCCACCAATCGTACCTGCAt         <  1:4181871/100‑1 (MQ=255)                                                                                    aaaCTAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGTTCCGATTAATGTACCCAAGCCACCAATCGTACCTGCAt         <  1:2559885/100‑1 (MQ=255)                                                                                    aaaCTAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGTTCCGATTAATGTACCCAAGCCACCAATCGTACCTGCAt         <  1:108179/100‑1 (MQ=255)                                                                                    aaaCTAATTTCATGTCCAAAATGTTGCATGTATTGCCCTTTTAAAATAATTAATGGCGGGGTTCCGATTAATGTACCCAAGCCACCAATCGTACCTGCAt         <  1:2885657/100‑1 (MQ=255)                                                                                       cTAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGTTCCGATTAATGTACCCAAGCCACCAATCGTACCTGCaggca      <  1:452511/100‑5 (MQ=255)                                                                                        tAATTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGTTCCGATTAATGTACCCAAGCCACCAATCGTACCTGCATAGcc     <  1:711739/100‑1 (MQ=255)                                                                                         aaTTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGTTCCGATTAATGTACCCAAGCCACCAATCGTACCTGCATAGCCa    <  1:1702310/100‑1 (MQ=255)                                                                                         aaTTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGTTCCGATTAATGTACCCAAGCCACCAATCGTACCTGCATAGCCa    <  1:1198019/100‑1 (MQ=255)                                                                                         aaTTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGTTCCGATTAATGTACCCAAGCCACCAATCGTACCTGCATAGCCa    <  1:1171397/100‑1 (MQ=255)                                                                                          aTTTCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGTTCCGATTAATGTACCCAAGCCACCAATCGTACCTGCATAGCCaa   <  1:4376914/100‑1 (MQ=255)                                                                                           tttCATGTCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGTTCCGATTAATGTACCCAAGCCACCAATCGTACCTGCATAGCCaag  <  1:4973432/100‑2 (MQ=255)                                                                                           tttCATGTCCAAAATGTTGCATGTATTGTCCTTTAAAAATAATTAATGGCGGGGTTCCGATTAATGTACCCAAGCCACCAATCGTACCTGCATAGCCAAt  <  1:5330593/100‑1 (MQ=255)                                                                                                 |                                                                                              TAAACGCAACATATCTTAAATAGAGCCAAGTAATACCTAACAAAACAATGACCGTTGGAATCCCTACAATCATCCATTTAGCAAAACTAATTTCATATCCAAAATGTTGCATGTATTGTCCTTTTAAAATAATTAATGGCGGGGTTCCGATTAATGTACCCAAGCCACCAATCGTACCTGCATAGCCAAT  >  NC_007793/2063226‑2063415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑