Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,057,596	T→C	I20V (ATT→GTT)	SAUSA300_RS10355 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,057,596	0	T	C	100.0%	45.3 / NA	19	I20V (ATT→GTT)	SAUSA300_RS10355	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/19);  total (0/19)

AAAACTAACTGATGCAGCAATATAACGATAACCTTTCAACGTTAAAATTGTTCGCATCGTTAAAAACGTTACATAACAAATGTTAATGATAAATATAGTTAGTACCATTAACCATGGATTTTCTGTTACGAATGACATAAGGCGCCCCACTCTCTTTCAATTAC  >  NC_007793/2057516‑2057679                                                                                 |                                                                                    aaaaCTAACTGATGCAGCAATATAACGATAACCTTTCAACGTTAAAATTGTTCGCATCGTTAAAAACGTTACATAACAAACGTTAATGATAAATAtagtt                                                                  <  1:2420791/100‑1 (MQ=255)       aactGATGCAGCAATATAACGATAACCTTTCAACGTTAAAATTGTTCGCATCGTTAAAAACGTTACATAACAAACGTTAATGATAAATATAGTTAGTAcc                                                            <  1:1662480/100‑1 (MQ=255)         ctGATGCAGCAATATAACGATAACCTTTCAACGTTAAAATTGTTCGCATCGTTAAAAACGTTACATAACAAACGTTAATGATAAATATAGTTAGTACCAt                                                          <  1:5094634/100‑1 (MQ=255)                 gcaATATAACGATAACCTTTCAACGTTAAAATTGTTCGCATCGTTAAAAACGTTACATAACAAACGTTAATGATAAATATAGTTAGTACCATTAACCAta                                                  <  1:4204734/100‑2 (MQ=255)                 gcaATATAACGATAACCTTTCAACGTTAAAATTGTGCGCATCGTTAAAAACGTTACATAACAAACGTTAATGATAAATATAGTTAGTACCATTAACCAtc                                                  <  1:5539437/100‑2 (MQ=255)                  caATATAACGATAACCTTTCAACGTTAAAATTGTTCGCATCGTTAAAAACGTTACATAACAAACGTTAATGATAAATATAGTTAGTACCATTAACCATgg                                                 <  1:3710304/100‑1 (MQ=255)                    atatAACGATAACCTTTCAACGTTAAAATTGTTCGCATCGTTAAAAACGTTACATAACAAACGTTAATGATAAATATAGTTAGTACCATTAACCATGGAt                                               <  1:4149104/100‑1 (MQ=255)                     tataACGATAACCTTTCAACGTTAAAATTGTTCGCATCGTTAAAAACGTTACATAACAAACGTTAATGATAAATATAGTTAGTACCATTAACCATGGAtt                                              <  1:3122768/100‑1 (MQ=255)                                ccTTTCAACGTTAAAATTGTTCGCATCGTTAAAAACGTTACATAACAAACGTTAATGATAAATATAGTTAGTACCATTAACCATGGATTTTCTGTTAcat                                   <  1:4905209/100‑3 (MQ=255)                                   ttCAACGTTAAAATTGTTCGCATCGTTAAAAACGTTACATAACAAACGTTAATGATAAATATAGTTAGTACCATTAACCATGGATTTTCTGTTACGAATg                                <  1:3132773/100‑1 (MQ=255)                                     cAACGTTAAAATTGTTCGCATCGTTAAAAACGTTACATAACAAACGTTAATGATAAATATAGTTAGTACCATTAACCATGGATTTTCTGTTACGAATGAc                              <  1:5219310/100‑1 (MQ=255)                                              aaTTGTTCGCATCGTTAAAAACGTTACATAACAAACGTTAATGATAAATATAGTTAGTACCATTAACCATGGATTTTCTGTTACGAATGACATAAGgagc                     <  1:327922/100‑1 (MQ=255)                                               aTTGTTCGCATCGTTAAAAACGTTACATAACAAACGTTAATGATAAATATAGTTAGTACCATTAACCATGGATTTTCTGTTACGAATGACATAAGgcgct                    <  1:3974667/100‑2 (MQ=255)                                                 tGTTCGCATCGTTAAAAACGTTACATAACAAACGTTAATGATAAATATAGTTAGTACCATTAACCATGGATTTTCTGTTACGAATGACATAAGgcgctgt                  <  1:1981944/100‑4 (MQ=255)                                                 tGTTCGCATCGTTAAAAACGTTACATAACAAACGTTAATGATAAATATAGTTAGTACCATTAACCATGGATTTTCTGTTACGAATGACATAAGGCGctcc                  <  1:5637307/100‑1 (MQ=255)                                                            ttAAAAACGTTACATAACAAACGTTAATGATAAATATAGTTAGTACCATTAACCATGGATTTTCTGTTACGAATGACATAAGGCGCTCCACTCTCTTTCa       <  1:551596/100‑1 (MQ=255)                                                               aaaaCGTTACATAACAAACGTTAATGATAAATATAGTTAGTACCATTAACCATGGATTTTCTGTTACGAATGACATAAGGCGCTCCACTCTCTTTCAAtt    <  1:3649668/100‑1 (MQ=255)                                                                 aaCGTTACATAACAAACGTTAATGATAAATATAGTTAGTACCATTAACCATGGATTTTCTGTTACGAATGACATAAGGCGCTCCACTCTCTTTCAATTac  <  1:4464551/100‑1 (MQ=255)                                                                 aaCGTTACATAACAAACGTTAATGATAAATATAGTTAGTACCATTAACCATGGATTTTCTGTTACGAATGACATAAGGCGCTCCACTCTCTTTCAATTac  <  1:2889/100‑1 (MQ=255)                                                                                 |                                                                                    AAAACTAACTGATGCAGCAATATAACGATAACCTTTCAACGTTAAAATTGTTCGCATCGTTAAAAACGTTACATAACAAATGTTAATGATAAATATAGTTAGTACCATTAACCATGGATTTTCTGTTACGAATGACATAAGGCGCCCCACTCTCTTTCAATTAC  >  NC_007793/2057516‑2057679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑