Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,034,247	G→T	intergenic (‑177/‑115)	SAUSA300_RS10245 ← / → SAUSA300_RS10250	UDP‑N‑acetylmuramate‑‑alanine ligase/non‑heme ferritin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,034,247	0	G	T	100.0%	17.3 / NA	8	intergenic (‑177/‑115)	SAUSA300_RS10245/SAUSA300_RS10250	UDP‑N‑acetylmuramate‑‑alanine ligase/non‑heme ferritin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (0/8);  total (0/8)

GTGTTTCTAAATTATAATCATTCTCATTTAGATAATTAAAAATGATATTTATTCTCAATTAAATATCGCTAAATGATACATTTTCTCAATTAGAGTGCTAAAATAAGGCATTAAATTATAATTATTATTATTTACAATTGAGAATGAGTTTGTTATACTA  >  NC_007793/2034155‑2034314                                                                                             |                                                                    gtgtTTCTAAATTATAATCATTCTCATTTAGATAATTAAAAATGGTATTTATTCTCAATTAAATATCGCTAAATGATACATTTTCTCAATTATAGTGCTa                                                              <  1:3530757/100‑1 (MQ=255) gtgtTTCTAAATTATAATCATTCTCATTTAGATAATTAAAAATGATATTTATTCTCAATTAAATATCGCTAAATGATACATTTTCTCAATTATAGTGcat                                                              <  1:5624075/100‑3 (MQ=255) gtgtTTCTAAATTATAATCATTCTCATTTAGATAATTAAAAATGATATTTATTCTCAATTAAATATCGCTAAATGATACATTTTCTCAATTATAGTGCTa                                                              <  1:1401296/100‑1 (MQ=255)   gtTTCTAAATTATAATCATTCTCATTTAGATAATTAAAAATGATATTTATTCTCAATTAAATATCGCTAAATGATACATTTTCTCAATTATAGTGCTaag                                                            <  1:3270915/100‑2 (MQ=255)          aaTTATAATCATTCTCATTTAGATAATTAAAAATGATATTTATTCTCAATTAAATATCGCTAAATGATACATTTTCTCAATTATAGTGCTAAAATAAGGc                                                     <  1:4250403/100‑1 (MQ=255)            ttATAATCATTCTCATTTAGATAATTAAAAATGATATTTATTCTCAATTAAATATCGCTAAATGATACATTTTCTCAATTATAGTGCTAAAATAAGGCAt                                                   <  1:3909782/100‑1 (MQ=255)            ttATAATCATTCTCATTTAGATAATTAAAAATGATATTTATTCTCAATTAAATATCGATAAATGATACATTTTCTCAATTATAGTGCTAAAATAAGGCag                                                   <  1:3427130/100‑2 (MQ=255)                                                             aaaTATCGCTAAATGATTCATTTTCTCAATTATAGTGCTAAAATAAGGCATTAAATTATAATTATTATTATTTACAATTGAGAATGAGTTTGTTATACTa  <  1:3181250/100‑1 (MQ=255)                                                                                             |                                                                    GTGTTTCTAAATTATAATCATTCTCATTTAGATAATTAAAAATGATATTTATTCTCAATTAAATATCGCTAAATGATACATTTTCTCAATTAGAGTGCTAAAATAAGGCATTAAATTATAATTATTATTATTTACAATTGAGAATGAGTTTGTTATACTA  >  NC_007793/2034155‑2034314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑