Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,019,080	C→A	intergenic (‑3/+128)	SAUSA300_RS10140 ← / ← SAUSA300_RS10145	glutamine amidotransferase/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,019,080	0	C	A	100.0%	88.1 / NA	31	intergenic (‑3/+128)	SAUSA300_RS10140/SAUSA300_RS10145	glutamine amidotransferase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (5/26);  total (5/26)

TATTAAAGCCTGCATTCTCTAATGCCTCTTTAGGGCTTGAATATTCTATATCTTCAAATTCGTTTGCTAGAATAATTGCTACTTTTTTAGTCATTGCAAATCACCTTTCTATATATCATTGATATAATTACTATAGACAAGTAAATCAGTGATTAAACATACAAGATATAAAAAATATTAAGCGACTGT  >  NC_007793/2018984‑2019172                                                                                                 |                                                                                             tATTAAAGCCTGCATTCTCTAATGCCTCTTTAGGGCTTGAATATTCTATATCTTCAAATTCGTTTGCTAGAATAATTGCTACTTTTTTAGTCATTGaaag                                                                                           <  1:5346188/100‑2 (MQ=255)       aGCCTGCATTCTCTAATGCCTCTTTAGGGCTTGAATATTCTATATCTTCAAATTCGTTTGCTAGAATAATTGCTACTTTTTTAGTCATTGAAAATCACCt                                                                                     <  1:5406838/100‑1 (MQ=255)       aGCCTGCATTCTCTAATGCCTCTTTAGGGCTTGAATATTCTATATCTTCAAATTCGTTTGCTAGAATAATTGCTACTTTTTTAGTCATTGAAAATCACCt                                                                                     >  1:2848676/1‑100 (MQ=255)           tGCATTCTCTAATGCCTCTTTAGGGCTTGAATATTCTATATCTTCAAATTCGTTTGCTAGAATAATTGCTACTTTTTTAGTCATTGAAAATCACCTTTCt                                                                                 <  1:743001/100‑1 (MQ=255)                  tctAATGCCTCTTTAGGGCTTGAATATTCTATATCTTCAAATTCGTTTGCTCGAATAATTGCTACTTTTTTAGTCATTGAAAATCACCTTTCTATATATc                                                                          <  1:2024535/100‑1 (MQ=255)                  tctAATGCCTCTTTAGGGCTTGAATATTCTATATCTTCAAATTCGTTTGCTAGAATAATTGCTACTTTTTTAGTCATTGAAAATCACTTTTCtatatcac                                                                          <  1:782617/100‑4 (MQ=255)                  tctAATGCCTCTTTAGGGCTTGAATATTCTATATCTTCAAATTCGTTTGCTAGAATAATTGCTACTTTTTTAGTCATTGAAAATCACCTTTCTATATATc                                                                          <  1:1841768/100‑1 (MQ=255)                   ctAATGCCTCTTTAGGGCTTGAATATTCTATATCTTCAAATTCGTTTGCTAGAATAATTGCTACTTTTTTAGTCATTGAAAATCACCTTTCTATATATCa                                                                         <  1:384631/100‑1 (MQ=255)                    tAATGCCTCTTTAGGGCTTGAATATTCTATATCTTCAAATTCGTTTGCTAGAATAATTGCTACTTTTTTAGTCATTGAAAATCACCTTTCTATATATCAt                                                                        <  1:2652324/100‑1 (MQ=255)                    tAATGCCTCTTTAGGGCTTGAATATTCTATATCTTCAAATTCGTTTGCTAGAATAATTGCTACTTTTTTAGTCATTGAAAATCACCTTTCTATATATCAt                                                                        <  1:2797854/100‑1 (MQ=255)                    tAATGCCTCTTTAGGGCTTGAATATTCTATATCTTCAAATTCGTTTGCTAGAATAATTGCTACTTTTTTAGTCATTGAAAATCACCTTTCTATATATCAt                                                                        <  1:3148528/100‑1 (MQ=255)                    tAATGCCTCTTTAGGGCTTGAATATTCTATATCTTCAAATTCGTTTGCTAGAATAATTGCTACTTTTTTAGTCATTGAAAATCACCTTTCTATATATCAt                                                                        <  1:4134995/100‑1 (MQ=255)                    tAATGCCTCTTTAGGGCTTGAATATTCTATATCTTCAAATTCGTTTGCTAGAATAATTGCTACTTTTTTAGTCATTGAAAATCACCTTTCTATATATCAt                                                                        <  1:4262526/100‑1 (MQ=255)                      aTGCCTCTTTAGGGCTTGAATATTCTATATCTTCAAATTCGTTTGCTAGAATAATTGCTACTTTTTTAGTCATTGAAAATCACCTTTCTATATATCATTg                                                                      <  1:2562719/100‑1 (MQ=255)                         ccTCTTTAGGGCTTGAATATTCTATATCTTCAAATTCGTTTGCTAGAATAATTGCTACTTTTTTAGTCATTGAAAATCACCTTTCTATATATCAtgcgca                                                                   <  1:5009990/100‑6 (MQ=255)                            ctTTAGGGCTTGAATATTCTATATCTTCAAATTCGTTTGCTAGAATAATTGCTACTTTTTTAGTCATTGAAACTCACCTTTCTATATATCATTGATATaa                                                                >  1:4355497/1‑100 (MQ=255)                               tAGGGCTTGAATATTCTATATCTTCAAATTCGTTTGCTAGAATAATTGCTACTTTTTTAGTCATTGAAAATCACCTTTCTATATATCATTGatatgcaca                                                             <  1:4712058/100‑6 (MQ=255)                                aGGGCTTGAATATTCTATATCTTCAAATTCGTTTGCTAGAATAATTGCTACTTTTTTAGTCATTGAAAATCACCTTTCTATATATCATTGatatgggcat                                                            <  1:373014/100‑7 (MQ=255)                                 gggCTTGAATATTCTATATCTTCAAATTCGTTTGCTAGAATAATTGCTACTTTTTTAGTCATTGAAAATCACCTTTCTATATATCATTGATATAATTACt                                                           <  1:387507/100‑1 (MQ=255)                                   gCTTGAATATTCTATATCTTCAAATTCGTTTGCTAGAATAATTGCTACTTTTTTAGTCATTGAAAATCACCTTTCTATATATCATTGATATAATTACtat                                                         <  1:719909/100‑1 (MQ=255)                                        aaTATTCTATATCTTCAAATTCGTTTGCTAGAATAATTGCTACTTTTTTAGTCATTGAAAATCACCTTTCTATATATCATTGATATAATTACTATAGACa                                                    <  1:2792846/100‑1 (MQ=255)                                          tatTCTATATCTTCAAATTCGTTTGCTAGAATAATTGCTACTTTTTTAGTCATTGAAAATCACCTTTCTATATATCATTGATATAATTACTATAGACAAg                                                  <  1:2226377/100‑1 (MQ=255)                                           atTCTATATCTTCAAATTCGTTTGCTAGAATAATTGCTACTTTTTTAGTCATTGAAAATCACCTTTCTATATATCATTGATATAATTACTATAGACAAGt                                                 <  1:3869893/100‑1 (MQ=255)                                                      tCAAATTCGTTTGCTAGAATAATTGCTACTTTTTTAGTCATTGAAAATCACCTTTCTATATATCATTGATATAATTACTATAGACAAGTAAATCAGTGAt                                      >  1:5645580/1‑100 (MQ=255)                                                                ttGCTAGAATAATTGCTACTTTTTTAGTCATTGAAAATCACCTTTCTATATATCATTGATATAATTACTATAGACAAGTAAATCAGTGATTAAACATACa                            <  1:5362078/100‑1 (MQ=255)                                                                  gCTAGAATAATTGCTACTTTTTTAGTCATTGAAAATCACCTTTCTATATATCATTGATATAATTACTATAGACAAGTAAATCAGTGATTAAACATACAAg                          <  1:3628755/100‑1 (MQ=255)                                                                    tAGAATAATTGCTACTTTTTTAGTCATTGAAAATCACCTTTCTATATATCATTGATATAATTACTATAGACAAGTAAATCAGTGATTAAACATACAAGat                        <  1:2010601/100‑1 (MQ=255)                                                                     aGAATAATTGCTACTTTTTTAGTCATTGAAAATCACCTTTCTATATATCATTGATTTAATTACTATAGACAAGTAAATCAGTGATTAAACATACAAGata                       >  1:4420034/1‑100 (MQ=255)                                                                     aGAATAATTGCTACTTTTTTAGTCATTGAAAATCACCTTTCTATATATCATTGATATAATTACTATAGACAAGTAAATCAGTGATTAAACATACAAGata                       >  1:601147/1‑100 (MQ=255)                                                                      gAATAATTGCTACTTTTTTAGTCATTGAAAATCACCTTTCTATATATCATTGATATAATTACTATAGACAAGTAAATCAGTGATTAAACATACAAGatat                      <  1:344174/100‑1 (MQ=255)                                                                        ataatTGCTACTTTTTTAGTCATTGAAAATCACCTTTCTATATATCATTGATATAATTACTATAGACAAGTAAATCAGTGATTAAACATACAAGATATaa                    <  1:1574144/100‑1 (MQ=255)                                                                                          gTCATTGAAAATCACCTTTCTATATATCATTGATATAATTACTATAGACAAGTAAATCAGTGATTAAACATACAAGATATAAAAAATATTAAGCGACTGt  <  1:2414131/100‑1 (MQ=255)                                                                                                 |                                                                                             TATTAAAGCCTGCATTCTCTAATGCCTCTTTAGGGCTTGAATATTCTATATCTTCAAATTCGTTTGCTAGAATAATTGCTACTTTTTTAGTCATTGCAAATCACCTTTCTATATATCATTGATATAATTACTATAGACAAGTAAATCAGTGATTAAACATACAAGATATAAAAAATATTAAGCGACTGT  >  NC_007793/2018984‑2019172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑