Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	2,015,166	C→T	L287L (TTG→TTA)	SAUSA300_RS10120 ←	teichoic acid ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	2,015,166	0	C	T	100.0%	210.9 / NA	74	L287L (TTG→TTA)	SAUSA300_RS10120	teichoic acid ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (1/73);  total (1/73)

CATCGTAGCACGATTCGTAAAATCTGTTATCGAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCCAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGTATTCTTGTACGA  >  NC_007793/2015067‑2015262                                                                                                    |                                                                                                 cATCGTAGCACGATTCGTAAAATCTGTTATCGAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGcc                                                                                                  <  1:687655/100‑1 (MQ=255)   tCGTAGCACGATTCGTAAAATCTGTTATCGAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTaa                                                                                                <  1:133871/100‑1 (MQ=255)   tCGTAGCACGATTCGTAAAATCTGTTATCGAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCGGAGGCTaa                                                                                                <  1:3291538/100‑1 (MQ=255)    cGTAGCACGATTCGTAAAATCTGTTATCGAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGccgcg                                                                                               <  1:1428524/100‑4 (MQ=255)     gTAGCACGATTCGTAAAATCTGTTATCGAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAg                                                                                              <  1:68460/100‑1 (MQ=255)        gCACGATTCGTAAAATCTTTTATCGAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTcc                                                                                           <  1:5725188/100‑1 (MQ=255)         cACGATTCGTAACATCTGTTATCGAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCa                                                                                          <  1:394840/100‑1 (MQ=255)         cACGATTCGTAAAATCTGTTATCGAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCa                                                                                          <  1:5212401/100‑1 (MQ=255)         cACGATTCGTAAAATCTGTTATCGAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCa                                                                                          <  1:1675388/100‑1 (MQ=255)         cACGATTCGTAAAATCTGTTATCGAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCa                                                                                          <  1:2973131/100‑1 (MQ=255)          aCGATTCGTAAAATCTGTTATCGAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTCCCTGAGGCTAAAGTCCag                                                                                         <  1:4705111/100‑2 (MQ=255)          aCGATTCGTAAAATCTGTTATCGAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCag                                                                                         <  1:2966909/100‑2 (MQ=255)          aCGATTCGTAAAATCTGTTATCGAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCaa                                                                                         <  1:1245650/100‑1 (MQ=255)          aCGATTCGTAAAATCTGTTATCGAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCaa                                                                                         <  1:993602/100‑1 (MQ=255)          aCGATTCGTAAAATCTGTTATCGAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCaa                                                                                         <  1:3026960/100‑1 (MQ=255)          aCGATTCGTAAAATCTGTTATCGAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCaa                                                                                         <  1:1392357/100‑1 (MQ=255)           cGATTCGTAAAATCTGTTATCGAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCaag                                                                                        <  1:2822448/100‑2 (MQ=255)           cGATTCGTAAAATCTGTTATCGAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCaag                                                                                        <  1:562285/100‑2 (MQ=255)           cGATTCGTAAAATCTGTTATCAAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCagg                                                                                        <  1:3804949/100‑3 (MQ=255)                 gTAAAATCTGTTATCGAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTcccgcaagtat                                                                                  <  1:5759805/100‑10 (MQ=255)                   aaaaTCTGTTATCGAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCAca                                                                                <  1:2686263/100‑2 (MQ=255)                     aaTCTGTTATCGAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTag                                                                              <  1:1300802/100‑2 (MQ=255)                     aaTCTGTTATCGAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTag                                                                              <  1:795837/100‑2 (MQ=255)                           ttATCGAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACtt                                                                        <  1:629472/100‑1 (MQ=255)                           ttATCGAAATAATTCCTATATTATTGAAAATGAGCAACACCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTaaccggga                                                                        <  1:689238/100‑7 (MQ=255)                             aTCGAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAg                                                                      <  1:4789728/100‑1 (MQ=255)                               cGAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGc                                                                    <  1:2882639/100‑1 (MQ=255)                                  aataatTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCgga                                                                 <  1:2388977/100‑2 (MQ=255)                                  aataatTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGt                                                                 <  1:4479712/100‑1 (MQ=255)                                   ataatTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGtg                                                                <  1:824177/100‑2 (MQ=255)                                   ataatTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGtc                                                                <  1:3239837/100‑2 (MQ=255)                                             tattatTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGAtt                                                      <  1:1856531/100‑1 (MQ=255)                                               ttattGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATtat                                                    <  1:346637/100‑1 (MQ=255)                                               ttattGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATtat                                                    <  1:3065919/100‑1 (MQ=255)                                               ttattGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATtat                                                    <  1:229269/100‑1 (MQ=255)                                               ttattGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATtaa                                                    <  1:5525796/100‑2 (MQ=255)                                                   tGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAgct                                                <  1:1032220/100‑2 (MQ=255)                                                    gAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAgcgt                                               <  1:3671728/100‑2 (MQ=255)                                                             cAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGt                                      <  1:5009221/100‑1 (MQ=255)                                                              aaCATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAacaagct                                     <  1:2322139/100‑5 (MQ=255)                                                              aaCATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGtt                                     <  1:3733600/100‑1 (MQ=255)                                                              aaCATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGta                                     <  1:1830225/100‑2 (MQ=255)                                                               aCATCATCAATTCTTAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGTTTTTGCATGATTATAGCGCTCAACACGttt                                    <  1:3566045/100‑1 (MQ=255)                                                                catcatCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTg                                   <  1:1909279/100‑1 (MQ=255)                                                                catcatCAATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTg                                   <  1:2169452/100‑1 (MQ=255)                                                                  tcatcaATGCTAAGCCTAAAATAGTGCCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGaa                                 <  1:1578298/100‑1 (MQ=255)                                                                  tcatcaATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGaa                                 <  1:139088/100‑1 (MQ=255)                                                                  gcatcaATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGaa                                 <  1:3538881/99‑1 (MQ=255)                                                                    atcaATGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGaaag                               <  1:2176873/100‑2 (MQ=255)                                                                       aaTGCTAAGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATtat                            <  1:38228/100‑1 (MQ=255)                                                                            tAAGCTTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTc                       <  1:3964642/100‑1 (MQ=255)                                                                             aaGCCTAAAATAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCa                      >  1:3245921/1‑100 (MQ=255)                                                                                   aaaaTAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTg                <  1:930933/100‑1 (MQ=255)                                                                                     aaTAGTACCTGAGGCTAAAGTCCAAAAACGCACTAGAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGt              <  1:3918506/100‑1 (MQ=255)                                                                                     aaTAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGt              <  1:586126/100‑1 (MQ=255)                                                                                     aaTAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGt              <  1:4242335/100‑1 (MQ=255)                                                                                     aaTAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGt              <  1:5670941/100‑1 (MQ=255)                                                                                     aaTAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGt              <  1:5475454/100‑1 (MQ=255)                                                                                     aaTAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGt              <  1:499334/100‑1 (MQ=255)                                                                                     aaTAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGt              <  1:3409645/100‑1 (MQ=255)                                                                                     aaTAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGt              <  1:4590353/100‑1 (MQ=255)                                                                                     aaTAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGt              <  1:4406761/100‑1 (MQ=255)                                                                                     aaTAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGt              <  1:4273417/100‑1 (MQ=255)                                                                                       tAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGTag            <  1:5342635/100‑2 (MQ=255)                                                                                       tAGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGTAt            <  1:1282089/100‑1 (MQ=255)                                                                                       tAGTAACTGAGGCTAAAGTCCAAAAACGAACTAAAAAATTAGCCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGTAt            <  1:5625417/100‑1 (MQ=255)                                                                                        aGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGTaga           <  1:5282285/100‑3 (MQ=255)                                                                                        aGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGTaac           <  1:4010160/100‑3 (MQ=255)                                                                                        aGTACCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGTAtg           <  1:3525467/100‑2 (MQ=255)                                                                                           aCCTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGTATTCtt        <  1:1763593/100‑1 (MQ=255)                                                                                             cTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGTATTCTTgg      <  1:4595715/100‑2 (MQ=255)                                                                                             cTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGTATTCTTGt      <  1:4757372/100‑1 (MQ=255)                                                                                             cTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGTATTCTTGt      <  1:824100/100‑1 (MQ=255)                                                                                             cTGAGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGTATTCTTGt      <  1:3157065/100‑1 (MQ=255)                                                                                                aGGCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGTATTCTTGTACg   <  1:2252995/100‑1 (MQ=255)                                                                                                 ggCTAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGTATTCTTGTAcac  <  1:3043227/100‑3 (MQ=255)                                                                                                    |                                                                                                 CATCGTAGCACGATTCGTAAAATCTGTTATCGAAATAATTCCTATATTATTGAAAATGAGCAACATCATCAATGCTAAGCCTAAAATAGTACCTGAGGCCAAAGTCCAAAAACGCACTAAAAACTTAGGCGGTTTTGCATGATTATAGCGCTCAACACGTTTGAAATTATAGGTCATCTCTGGTATTCTTGTACGA  >  NC_007793/2015067‑2015262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑