Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007793 2,004,460 C→A L166L (CTG→CTT)  SAUSA300_RS10065 ← 2‑hydroxyacid dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077932,004,4600CA99.4% 502.0 / ‑4.6 168L166L (CTG→CTT) SAUSA300_RS100652‑hydroxyacid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (3/164);  total (3/165)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01

Only 100 of 168 total aligned reads displayed.

AAGCATTTATAATAATGTCAGCATTTGGTAATGTGCTTTCAAGTGATTCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTCAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAATTGCACCTGTACCTAAAAATAAAACTGTTTGTCCTGATAGG  >  NC_007793/2004363‑2004556
                                                                                                 |                                                                                                
aaGCATTTATAATAATGTCAGCATTTGGTAATGTGCTTTCAAGTGATTCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAg                                                                                                <  1:4694960/100‑1 (MQ=255)
aaGCATTTATAATAATGTCAGCATTTGGTAATGTGCTTTCAAGTGATTCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAg                                                                                                <  1:349032/100‑1 (MQ=255)
aaGCATTTATAATAATGTCAGCATTTGGTAATGTGCTTTCAAGTGATTCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAg                                                                                                <  1:1817252/100‑1 (MQ=255)
   cATTTATAATAATGTCAGCATTTGGTAATGTGCTTTCAAGTGATTCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGAcc                                                                                             <  1:2372567/100‑1 (MQ=255)
      ttATAATAATGTCAGCATTTGGTAATGTGCTTTCAAGTGATTCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGAccggt                                                                                          <  1:4561274/100‑4 (MQ=255)
      ttATAATAATGTCAGCATTTGGTAATGTGCTTTCAAGTGATTCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCagt                                                                                          <  1:344200/100‑3 (MQ=255)
                             aaTGTGCTTTCAAGTGATTCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCttgt                                                                   <  1:2168110/100‑3 (MQ=255)
                             aaTGTGCTTTCAAGTGATTCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTg                                                                   <  1:408026/100‑1 (MQ=255)
                              aTGTGCTTTCAAGTGATTCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTgt                                                                  <  1:4212835/100‑2 (MQ=255)
                               tgtgCTTTCAAGTGATTCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCt                                                                 <  1:2073606/100‑1 (MQ=255)
                                gtgCTTTCAAGTGATTCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTa                                                                <  1:1061304/100‑1 (MQ=255)
                                gtgCTTTCAAGTGATTCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTa                                                                <  1:5706239/100‑1 (MQ=255)
                                gtgCTTTCAAGTGATTCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTa                                                                <  1:4332873/100‑1 (MQ=255)
                                       cAAGTGATTCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGc                                                         <  1:3510741/100‑1 (MQ=255)
                                       cAAGTGATTCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGc                                                         <  1:2347944/100‑1 (MQ=255)
                                       cAAGTGATTCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGc                                                         <  1:2402415/100‑1 (MQ=255)
                                       cAAGTGATTCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGc                                                         <  1:2690704/100‑1 (MQ=255)
                                       cAAGTGATTCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGc                                                         <  1:2955198/100‑1 (MQ=255)
                                       cAAGTGATTCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGc                                                         <  1:830404/100‑1 (MQ=255)
                                       cAAGTGATTCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGc                                                         <  1:4561199/100‑1 (MQ=255)
                                       cAAGTGATTCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGc                                                         <  1:4877371/100‑1 (MQ=255)
                                       cAAGTGATTCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGc                                                         <  1:727513/100‑1 (MQ=255)
                                       cAAGTGATTCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGc                                                         <  1:1721035/100‑1 (MQ=255)
                                       cAAGTGATTCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGc                                                         <  1:1170247/100‑1 (MQ=255)
                                       cAAGTGATTCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCCTTGCTAACTTCGc                                                         <  1:4523080/100‑1 (MQ=255)
                                       cAAGTGATTCGATAGTATATACCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGc                                                         <  1:231415/100‑1 (MQ=255)
                                        aaGTGATTCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCa                                                        <  1:4405383/100‑1 (MQ=255)
                                         aGTGATTCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAg                                                       <  1:5366681/100‑1 (MQ=255)
                                         aGTGATTCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCGGACTTGCTAAGACCAAAAAAATTCATATTAAAAGCCTTTGCTAACTTCGCAg                                                       <  1:1867752/100‑1 (MQ=255)
                                          gTGATTCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAgg                                                      <  1:5170397/100‑2 (MQ=255)
                                          gTGATTCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGt                                                      <  1:1313967/100‑1 (MQ=255)
                                           gatctCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGtt                                                     <  1:1456229/96‑1 (MQ=255)
                                             tctCGGTAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCt                                                   <  1:5686935/98‑1 (MQ=255)
                                             aTTCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAGTTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCt                                                   <  1:1927452/100‑1 (MQ=255)
                                             aTTCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGttgg                                                   <  1:3257721/100‑3 (MQ=255)
                                             aTTCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCt                                                   <  1:2651105/100‑1 (MQ=255)
                                             aTTCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCt                                                   <  1:5301651/100‑1 (MQ=255)
                                             aTTCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCt                                                   <  1:5734537/100‑1 (MQ=255)
                                             aTTCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACATGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCt                                                   <  1:697689/100‑1 (MQ=255)
                                             aTTCGATAGTATATATCTCGTCAAACTCATCATTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCt                                                   <  1:4582526/100‑1 (MQ=255)
                                              ttCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCta                                                  <  1:1693333/100‑1 (MQ=255)
                                                cGATAGTATATATCTCGTCAAACTCGTCTTTGTTCTGACCTTGAGTGATAAGACAAATTAAAGTCATAGTAAAAGCCTTTGCGAACTTCGCAGTTCtagt                                                <  1:3926945/100‑1 (MQ=255)
                                                cGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGGCCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCtagt                                                <  1:2839196/100‑1 (MQ=255)
                                                cGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCtagt                                                <  1:4158781/100‑1 (MQ=255)
                                                cGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCtagt                                                <  1:4861765/100‑1 (MQ=255)
                                                cGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCtagt                                                <  1:476633/100‑1 (MQ=255)
                                                cGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCtagt                                                <  1:5766172/100‑1 (MQ=255)
                                                cGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCtagt                                                <  1:5779545/100‑1 (MQ=255)
                                                cGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCtagt                                                <  1:3587848/100‑1 (MQ=255)
                                                cGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCtagt                                                <  1:1011805/100‑1 (MQ=255)
                                                cGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCtagt                                                <  1:1732314/100‑1 (MQ=255)
                                                cGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCtagt                                                <  1:3374516/100‑1 (MQ=255)
                                                cGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCtagt                                                <  1:2125567/100‑1 (MQ=255)
                                                cGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCtagt                                                <  1:2399947/100‑1 (MQ=255)
                                                cGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCtagt                                                <  1:176156/100‑1 (MQ=255)
                                                cGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCtagg                                                <  1:2804013/100‑2 (MQ=255)
                                                cGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTCCtagt                                                <  1:5569771/100‑1 (MQ=255)
                                                 aaTAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCtagta                                               >  1:2900543/2‑100 (MQ=255)
                                                   tAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGc                                             <  1:374541/100‑1 (MQ=255)
                                                    aGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCtacccttg                                            <  1:4611013/100‑7 (MQ=255)
                                                     gTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCCTTGCTAACTTCGCAGTTCTAGTAGCag                                           <  1:5133772/100‑2 (MQ=255)
                                                      tatataTCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAAt                                          <  1:814711/100‑1 (MQ=255)
                                                       atatatCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCaagg                                         <  1:189951/100‑3 (MQ=255)
                                                             ctcGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAATTGCAcca                                   <  1:2959873/100‑2 (MQ=255)
                                                              tcGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAATTGCACCTg                                  <  1:2102830/100‑1 (MQ=255)
                                                              tcGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAATTGCACCTg                                  <  1:1338461/100‑1 (MQ=255)
                                                               cGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAATTGCACCTGt                                 <  1:1527919/100‑1 (MQ=255)
                                                               cGTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAATTGCACCTGt                                 <  1:4641670/100‑1 (MQ=255)
                                                                gTCAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAATTGCACCTGTa                                <  1:4271496/100‑1 (MQ=255)
                                                                  cAAACTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAATTGCACCTGTAcc                              <  1:2521076/100‑1 (MQ=255)
                                                                   aaaCTCATCTTTGTTCTGACCTGACTTGCTAAGGCCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAATTGCACCTGTACCt                             <  1:5720089/100‑1 (MQ=255)
                                                                   aaaCTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAATTGCACCTGTACCt                             <  1:5208192/100‑1 (MQ=255)
                                                                   aaaCTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAATTGCACCTGTACCt                             <  1:3936400/100‑1 (MQ=255)
                                                                   aaaCTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAATTGCACCTGTACCt                             <  1:5017965/100‑1 (MQ=255)
                                                                   aaaCTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAATTGCACCTGTACCt                             <  1:3982013/100‑1 (MQ=255)
                                                                    aaCTCATCTTTGTTCTGACCTGGCTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAATTGCACCTGTACCTa                            <  1:2211196/100‑1 (MQ=255)
                                                                    aaCTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAATTGCACCTGTACCTa                            <  1:637806/100‑1 (MQ=255)
                                                                    aaCTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAATTGCACCTGTACCTa                            <  1:1287904/100‑1 (MQ=255)
                                                                    aaCTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAATTGCACCTGTACCTa                            <  1:2906758/100‑1 (MQ=255)
                                                                    aaCTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAATTGCACCTGTACCTa                            <  1:4356827/100‑1 (MQ=255)
                                                                     aCTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAATTGCACCTGTACCtca                           <  1:4886541/100‑3 (MQ=255)
                                                                      cTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAATTGCACCTGTACCTaag                          <  1:1781266/100‑2 (MQ=255)
                                                                      cTCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAATTGCACCTGTACCTaac                          <  1:2340757/100‑2 (MQ=255)
                                                                       tCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAATTGCACCTGTACCTaaaa                         <  1:2951854/100‑1 (MQ=255)
                                                                       tCATCTTTGTTCTGACCTGACTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAATTGCACCTGTACCTaaaa                         <  1:5397955/100‑1 (MQ=255)
                                                                                  ctgacctgacTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAATTGCACCTGTACCTAAAAATAAAACTGtt              <  1:1883607/100‑1 (MQ=255)
                                                                                   tgacctgacTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAATTGCACCTGTACCTAAAAATAAAACTGttt             <  1:3676765/100‑1 (MQ=255)
                                                                                        tgacTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAATTGCACCTGTACCTAAAAATAAAACTGTTTGTCCt        <  1:2561415/100‑1 (MQ=255)
                                                                                         gacTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAATTGCACCTGTACCTAAAAATAAAACTGTTTGTcccg       <  1:3357459/100‑3 (MQ=255)
                                                                                         gacTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAATTGCACCTGTACCTAAAAATAAAACTGTTTGTCCTg       >  1:5574811/1‑100 (MQ=255)
                                                                                         gacTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAATTGCACCTGTACCTAAAAATAAAACTGTTTGTCCTg       <  1:3465287/100‑1 (MQ=255)
                                                                                          gactGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAATTGCACCTGTACCTAAAAATAAAACTGTTTGTCCTgg      <  1:1581158/97‑2 (MQ=255)
                                                                                          acTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAATTGCACCTGTACCTAAAAATAAAACTGTTTGTCCTGa      <  1:412778/100‑1 (MQ=255)
                                                                                           ctggCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAATTGCACCTGTACCTAAAAATAAAACTGTTTGTCCTGAt     <  1:1046996/97‑1 (MQ=255)
                                                                                           cctGCTAAGTCCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAATTGCACCTGTACCTAAAAATAAAACTGTTTGTCCTGAt     <  1:47051/98‑1 (MQ=255)
                                                                                           cTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAATTGCACCTGTACCTAAAAATAAAACTGTTTGTCCTGAt     <  1:2042806/100‑1 (MQ=255)
                                                                                           cTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAATTGCACCTGTACCTAAAAATAAAACTGTTTGTCCTGAt     <  1:2543652/100‑1 (MQ=255)
                                                                                           cTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAATTGCACCTGTACCTAAAAATAAAACTGTTTGTCCTGAt     <  1:1224283/100‑1 (MQ=255)
                                                                                           cTTGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAAATGCACCTGTACCTAAAAATAAAACTGTTTGTCCTGAt     <  1:4784317/100‑1 (MQ=255)
                                                                                           actGCTAAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAATTGCACCTGTACCTAAAAATAAAACTGTTTGTCCTGAt     <  1:3795950/98‑1 (MQ=255)
                                                                                                 |                                                                                                
AAGCATTTATAATAATGTCAGCATTTGGTAATGTGCTTTCAAGTGATTCGATAGTATATATCTCGTCAAACTCATCTTTGTTCTGACCTGACTTGCTCAGACCAATTAAATTCATATTAAAAGCCTTTGCTAACTTCGCAGTTCTAGTAGCAATTGCACCTGTACCTAAAAATAAAACTGTTTGTCCTGATAGG  >  NC_007793/2004363‑2004556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑