Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	47,278	T→G	V81F (GTA→TTC)	SAUSA300_RS00200 ←	recombinase RecB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	47,278	0	T	G	100.0%	41.6 / NA	17	V81V (GTA→GTC)	SAUSA300_RS00200	recombinase RecB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (12/5);  total (12/5)

TTTGTTTGTGTAAATCTTCGACAATTTTGAGAAGATCAGATGTATTACGAGCTAATCGGTTTGTTTTGTATACCATAACACAGTCGATATTGCCTTCTTTTGCATCTTTCAACATACGTTGGAGCTCAGGTCTGTTCATAGATTTACCTGAAATACCACGGTCAGC  >  NC_007793/47208‑47373                                                                       |                                                                                                tttgtttaTGCAAATCTTCGACGATTTTCAGAAGATCCGATGTATTACGAGCTAATCGGTTTGTTTTGTAGATCATAACACAATCTATATTGCCTTCttt                                                                    >  1:1696655/1‑100 (MQ=255)        atgcaaaTCTTCGACGATTTTCAGAAGATCCGATGTATTACGAGCTAATCGGTTTGTTTTGTAGATCATAACACAATCTATATTGCCTTCTTTTGCAtac                                                             <  1:118557/96‑3 (MQ=255)                                     ccgATGTATTACGAGCTAATCGGTTTGTTTTGTAGATCATAACACAATCTATATTGCCTTCTTTTGCATCTTTCAACATACGTTGGAGCTCAGGTCTGtt                                >  1:2573188/3‑100 (MQ=255)                                      ccaTGTATTACGAGCTAATCGGTTTGTTTTGTAGATCATAACACAATCTATATTGCCTTCTTTTTCATCTTTCAACATACGTTGGAGCTCAGGTCTGTTc                               >  1:234237/3‑100 (MQ=255)                                        ttGTATTACGAGCTAATCGGTTTGTTTTGTAGATCATAACACAATCTATATTGCCTTCTTTTGCATCTTTCAACATACGTTGGAGCTCAGGTCTGTTCAt                             >  1:3759972/2‑100 (MQ=255)                                        aTGTATTACGAGCTAATCGGTTTGTTTTGTAGATCATAACACAATCTATATTGCCTTCTTTTGCATCTTTCAACATACGTTGGAGCTCAGGTCTGTTCAt                             <  1:4991078/100‑1 (MQ=255)                                        aTGTATTACGAGCTAATCGGTTTGTTTTGTAGATCATAACACAATCTATATTGCCTTCTTTTGCATCTTTCAACATACGTTGGAGCTCAGGTCTGTTCAt                             <  1:2785869/100‑1 (MQ=255)                                            aTTACGAGCTAATCGGTTTGTTTTGTAGATCATAACACAATCTATATTGCCTTCTTTTGCATCTTTCAACATACGTTGGAGCTCAGGTCTGTTCATAGAt                         >  1:4953004/1‑100 (MQ=255)                                            aTTACGAGCTAATCGGTTTGTTTTGTAGATCATAACACAATCTATATTGCCTTCTTTTGCATCTTTCAACATACGTTGGAGCTCAGGTCTGTTCATAGAt                         >  1:4818164/1‑100 (MQ=255)                                             ccaCGAGCTAATCGGTTTGTTTTGTAGATCATAACACAATCTATATTGCCTTCTTTTGCATCTTTCAACATACGTTGGAGCTCAGGTCTGTTCATAGAtt                        >  1:1142688/3‑100 (MQ=255)                                                   gCTAATCGGTTTGTTTTGTAGATCATAACACAATCTATATTGCCTTCTTTTGCATCTTTCAACATACGTTGGAGCTCAGGTCTGTTCATAGATTTACCTg                  >  1:5069427/1‑100 (MQ=255)                                                      aaTCGGTTTGTTTTGTAGATCATAACACAATCTATATTGCCTTCTTTTGCATCTTTCAACATACGTTGGAGCTCAGGTCTGTTCATAGATTTACCTGaaa               >  1:4006212/1‑100 (MQ=255)                                                      aaTCGGTTTGTTTTGTAGATCATAACACAATCTATATTGCCTTCTTTTGCATCTTTCAACATACGTTGGAGCTCAGGTCTGTTCATAGATTTACCTGaaa               >  1:2311607/1‑100 (MQ=255)                                                         cGGTTTGTTTTGTAGATCATAACACAATCTATATTGCCTTCTTTTGCATCTTTCAACATACGTTGGAGCTCAGGTCTGTTCATAGATTTACCTGAAATAc            >  1:530363/1‑100 (MQ=255)                                                             ttgttttgtAGATCATAACACAATCTATATTGCCTTCTTTTGCATCTTTCAACATACGTTGGAGCTCAGGTCTGTTCATAGATTTACCTGAAATACCACg        >  1:1444927/1‑100 (MQ=255)                                                              tgttttgtAGATCATAACACAATCTATATTGCCTTCTTTTGCATCTTTCAACATACGCTGGAGCTCAGGTCTGTTCATAGATTTACCTGAAATACCAcca       <  1:1056822/100‑3 (MQ=255)                                                                   tgtAGATCATAACACAATCTATATTGCCTTCTTTTGCATCTTTCAACATACGTTGGAGCTCAGGTCTGTTCATAGATTTTCCTGAAATACCACGATCAGc  <  1:5582081/100‑1 (MQ=255)                                                                       |                                                                                                TTTGTTTGTGTAAATCTTCGACAATTTTGAGAAGATCAGATGTATTACGAGCTAATCGGTTTGTTTTGTATACCATAACACAGTCGATATTGCCTTCTTTTGCATCTTTCAACATACGTTGGAGCTCAGGTCTGTTCATAGATTTACCTGAAATACCACGGTCAGC  >  NC_007793/47208‑47373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑