Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,945,182	C→G	N183K (AAC→AAG)	SAUSA300_RS09630 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,945,182	0	C	G	100.0%	42.1 / NA	17	N183K (AAC→AAG)	SAUSA300_RS09630	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (6/11);  total (6/11)

TTCTCCAGTCTCGGGTAAAGTGGGAGAAGGAGTAACTAGTGGTGGCGAAAATACTCTAACTAAACGCTTCATCAACAAAATCACAATTGATTAAACCTTAAACTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCACCTAGCCCAT  >  NC_007793/1945107‑1945272                                                                            |                                                                                           atctcCAGTCTCGGGTAAAGTGGGAGAAGGAGTAACTAGTGGTGGCGAAAATACTCTAACTAAACGCTTCATCAAGAAAATCACAATTGATTAAACCTTa                                                                    >  1:2784789/2‑100 (MQ=255)  tttccgatctcGGGTAAAGTGGGAGAAGGAGTAACTAGTGGTGGCGAAAATACTCTAACTAAACGCTTCATCAAGAAAATCACAATTGATTAAACCTTaa                                                                   <  1:1306807/93‑1 (MQ=255)          ctctGGTAAAGTGGGAGAAGGAGTAACTAGTGGTGGCGAAAATACTCTAACTAAACGCTTCATCAAGAAAATCACAATTGATTAAACCTTAAACTCaagc                                                           <  1:5786009/100‑3 (MQ=255)            cGGGTAAAGTGGGAGAAGGAGTAACTAGTGGTGGCGAAAATACTCTAACTAAACGCTTCATCAAGAAAATCACAATTGATTAAACCTTAAACTCAAAttt                                                         >  1:2514976/1‑100 (MQ=255)             gggTAAAGTGGGAGAAGGAGTAACTAGTGGTGGCGAAAATACTCTAACTAAACGCTTCATCAAGAAAATCACAATTGATTAAACCTTAAACTCAAATTTc                                                        >  1:3201968/1‑100 (MQ=255)                     tGGGAGAAGGAGTAACTAGTGGTGGCGAAAATACTCTAACTAAACGCTTCATCAAGAAAATCACAATTGATTAAACCTTAAACTCAAATTTCCTTAATTc                                                >  1:3077120/1‑100 (MQ=255)                                         ggtggCGAAAATACTCTAACTAAACGCTTCATCAAGAAAATCACAATTGATTAAACCTTAAACTCAAATTTCCTTAATTCATAAAACATTAAtaactaac                            >  1:1996902/1‑100 (MQ=255)                                               gAAAATACTCTAACTAAACGCTTCATCAAGAAAATCACAATTGATTAAACCTTAAACTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTc                      <  1:3704550/100‑1 (MQ=255)                                                        ctaactaaACGCTTCATCAAGAAAATCACAATTGATTAAACCTTAAACTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAacc             <  1:1629081/100‑1 (MQ=255)                                                        ctaactaaACGCTTCATCAAGAAAATCACAATTGATTAAACCTTAAACTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAacc             >  1:3814435/1‑100 (MQ=255)                                                            ctaaACGCTTCATCAAGAAAATCACAATTGATTAAACCTTAAACTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCACCt         <  1:3004086/100‑1 (MQ=255)                                                            ctaaACGCTTCATCAAGAAAATCACAATTGATTAAACCTTAAACTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCACCt         <  1:3639746/100‑1 (MQ=255)                                                            ctaaACGCTTCATCAAGAAAATCACAATTGATTAAACCTTAAACTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCACCt         <  1:3792556/100‑1 (MQ=255)                                                              aaaCGCTTCATCAAGAAAATCACAATTGATTAAACCTTAAACTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCACCtga       <  1:3509760/100‑3 (MQ=255)                                                                 cGCTTCATCAAGAAAATCACAATTGATTAAACCTTAAACTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCACCTAGccc    <  1:5439289/100‑1 (MQ=255)                                                                   cTTCATCAAGAAAATCACAATTGATTAAACCTTAAACTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCACCTAGCCCat  <  1:1768821/100‑1 (MQ=255)                                                                   cTTCATCAAGAAAATCACAATTGATTAAACCTTAAACTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCACCTAGCCCaa  <  1:1107152/100‑2 (MQ=255)                                                                            |                                                                                           TTCTCCAGTCTCGGGTAAAGTGGGAGAAGGAGTAACTAGTGGTGGCGAAAATACTCTAACTAAACGCTTCATCAACAAAATCACAATTGATTAAACCTTAAACTCAAATTTCCTTAATTCATAAAACATTAATAACTAACCCTTTCTTACAAACCACCTAGCCCAT  >  NC_007793/1945107‑1945272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑