Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,940,234	A→G	A36A (GCT→GCC)	SAUSA300_RS09600 ←	serine protease SplE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,940,234	0	A	G	100.0%	15.8 / NA	8	A36A (GCT→GCC)	SAUSA300_RS09600	serine protease SplE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (1/7);  total (1/7)

CTATCGAAACGACACCATTGTATGGTGCTACATTAGTATTTTTGATTAGTTTCACATTATGTTCAGCTTTAGCCGTTTGTTGAATACCCTCAACCACTGTTGTGCCGACGCCAGTCACTGATG  >  NC_007793/1940170‑1940292                                                                 |                                                           tcttCGAAACGACACCATTGTATGGTGCTACATTAGTATTTTTGATTAGTTTCACATTATGTTCGGCTTTAGCCGTTTGTTGAATACCCTCAACCACtgt                         <  1:1745190/97‑1 (MQ=255) cTATCGAAACGACACCATTGTATGGTGCTACATTAGTATTTTTGATTAGTTTCACATTATGTTCGGCTTTAGCCGTTTGTTGAATACCCTCAACCACtgt                         <  1:3338137/100‑1 (MQ=255) cTATCGAAACGACACCATTGTATGGTGCTACATTAGTATTTTTGATTAGTTTCACATTATGTTCGGCTTTAGCCGTTTGTTGAATACCCTCAACCACtga                         <  1:4276819/100‑2 (MQ=255)        aaCGACACCATTGTATGGTGCTACATTAGTATTTTTGATTAGTTTCACATTATGTTCGGCTTTAGCCGTTTGTTGAATACCCTCAACCACTGTTGTGCCg                  <  1:2944563/100‑1 (MQ=255)         aCGACACCATTGTATGGTGCTACATTAGTATTTTTGATTAGTTTCACATTATGTTCGGCTTTAGCCGTTTGTTGAATACCCTCAACCACTGTTGTGCCgt                 <  1:4698277/100‑2 (MQ=255)             cacCATTGTATGGTGCTACATTAGTATTTTTGATTAGTTTCACATTATGTTCGGCTTTAGCCGTTTGTTGAATACCCTCAACCACTGTTGTGCCGACGcc             <  1:5335089/100‑1 (MQ=255)                 aTTGTATGGTGCTACATTAGTATTTTTGATTAGTTTCACATTATGTTCGGCTTTAGCCGTTTGTTGAATACCCTCAACCACTGTTGTGCCGACGCCAgag         <  1:1586996/100‑3 (MQ=255)                        ggTGCTACATTAGTATTTTTGATTAGTTTCACATTATGTTCGGCTTTAGCCGTTTGTTGAATACCCTCAACCACTGTTGTGCCGACGCCAGTCACTGATg  >  1:4924462/1‑100 (MQ=255)                                                                 |                                                           CTATCGAAACGACACCATTGTATGGTGCTACATTAGTATTTTTGATTAGTTTCACATTATGTTCAGCTTTAGCCGTTTGTTGAATACCCTCAACCACTGTTGTGCCGACGCCAGTCACTGATG  >  NC_007793/1940170‑1940292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑