Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,924,930	T→C	intergenic (+229/‑3)	SAUSA300_RS09515 → / → SAUSA300_RS09520	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,924,930	0	T	C	100.0%	74.1 / NA	29	intergenic (+229/‑3)	SAUSA300_RS09515/SAUSA300_RS09520	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/1);  total (28/1)

GCTAATCAACATAAAGAAAATAGTAGTAAATCAAATGACACTAATAAAAAGACGCAACAAACTGACAACACTACACAGTCAAATACAGAAAAGTAAATGACACCACAAGAAGCCGAAGATATAGTTCGAAACGATTACAAAGCAAGAGGCGTTAATGAATATCAAACATTAAATTATAAAACAAATCTT  >  NC_007793/1924837‑1925025                                                                                              |                                                                                                gCTAATCAACATAAAGAAAATAGTAGTAAATCAAATGACACTAATAAAAAGACGCAACAAACTGACAACACTACACAGTCAAATACAGAAAAGCAAATGa                                                                                           >  1:4918728/1‑100 (MQ=255) gCTAATCAACATAAAGAAAATAGTAGTAAATCAAATGACACTAATAAAAAGACGCAACAAACTGACAACACTACACAGTCAAATACAGAAAAGCAAATGa                                                                                           >  1:3535293/1‑100 (MQ=255)    caTCAACATAAAGAAAATAGTAGTAAATCAAATGACACTAATAAAAAGACGCAACAAACTGACAACACTACACAGTCAAATACAGAAAAGCAAATGacac                                                                                        >  1:5392191/2‑100 (MQ=255)    aaTCAACATAAAGAAAATAGTAGTAAATCAAATGACACTAATAAAAAGACGCAACAAACTGACAACACTACACAGTCAAATACAGAAAAGCaaagatcgg                                                                                        >  1:3350631/1‑94 (MQ=255)    aaTCAACATAAAGAAAATAGTAGTAAATCAAATGACACTAATAAAAAGACGCAACAAACTGACAACACTACACAGTCAAATACAGAAAAGCAAATGacgc                                                                                        >  1:5323469/1‑98 (MQ=255)    aaTCAACATAAAGAAAATAGTAGTAAATCAAATGACACTAATAAAAAGACGCAACAAACTGACAACACTACACAGTCAAATACAGAAAAGCAAATGacac                                                                                        >  1:2508422/1‑100 (MQ=255)    aaTCAACATAAAGAAAATAGTAGTAAATCAAATGACACTAATAAAAAGACGCAACAAACTGACAACACTACACAGCCAAATACAGAAAAGCAAATGacac                                                                                        >  1:4910449/1‑100 (MQ=255)     aTCAACATAAAGAAAATAGTAGTAAATCAAATGACACTAATAAAAAGACGCAACAAACTGACAACACTACACAGTCAAATACAGAAAAGCAAATGAcacc                                                                                       >  1:2760891/1‑100 (MQ=255)       ctaCATAAAGAAAATAGTAGTAAATCAAATGACACTAATAAAAAGACGCAACAAACTGACAACACTACACAGTCAAATACAGAAAAGCAAATGAcaccac                                                                                     >  1:5468653/3‑100 (MQ=255)               aGAAAATAGTAGTAAATCAAATGACACTAATAAAAAGACGCAACAAACTGACAACACTACACAGTCAAATACAGAAAAGCAAATGACACCACaagatcga                                                                             >  1:1374329/1‑96 (MQ=255)                 aaaaTAGTAGTAAATCAAATGACACTAATAAAAAGACGCAACAAACTGACAACACTACACAGTCAAATACAGAAAAGCAAATGACACCACAAGAAGCCgg                                                                           <  1:1956669/100‑2 (MQ=255)                         agtaAATCAAATGACACTAATAAAAAGACGCAACAAACTGACAACACTACACAGTCAAATACAGAAAAGCAAATGACACCACAAGAAGCCGAAGatcgga                                                                   >  1:2164212/1‑96 (MQ=255)                                 aaaTGACACTAATAAAAAGACGCAACAAACTGACAACACTACACAGTCAAATACAGAAAAGCAAATGACACCACAAGAAGCCGAAGATATAGTTCGAAAc                                                           >  1:3470731/1‑100 (MQ=255)                                   aTGACACTAATAAAAAGACGCAACAAACTGACAACACTACACAGTCAAATACAGAAAAGCAAATGACACCACAAGAAGCCGAAGATATAGTTCGAAACGa                                                         >  1:4696513/1‑100 (MQ=255)                                     gACACTAATAAAAAGACGCAACAAACTGACAACACTACACAGTCAAATACAGAAAAGCAAATGACACCACAAGAAGCCGAAGATATAGTTCGAAACGAtt                                                       >  1:3914148/1‑100 (MQ=255)                                        ccTAATAAAAAGACGCAACAAACTGACAACACTACACAGTCAAATACAGAAAAGCAAATGACACCACAAGAAGCCGAATATATAGTTCGAAACGATTACa                                                    >  1:1023717/2‑100 (MQ=255)                                           aataaAAAGACGCAACAAACTGACAACACTACACAGTCAAATACAGAAAAGCAAATGACACCACAAGAAGCCGAAGATATAGTTCGAAACGATTACAAAg                                                 >  1:36813/1‑100 (MQ=255)                                                                gACAACACTACACAGTCAAATACAGAAAAGCAAATGACACCACAAGAAGCCGAAGATATAGTTCGAAACGATTACAAAGCAAGAGGCGTTAATGAATATc                            >  1:3328511/1‑100 (MQ=255)                                                                gACAACACTACACAGTCAAATACAGAAAAGCAAATGACACCACAAGAAGCCGAAGATATAGTTCGAAACGATTACAAAGCAAGAGGCGTTAATGAATATc                            >  1:2647070/1‑100 (MQ=255)                                                                gACAACACTACACAGTCAAATACAGAAAAGCAAATGACACCACAAGAAGCCGAAGATATAGTTCGAAACGATTACAAAGCAAGAGGCGTTAATGAATATc                            >  1:1581092/1‑100 (MQ=255)                                                                gACAACACTACACAGTCAAATACAGAAAAGCAAATGACACCACAAGAAGCCGAAGATATAGTTCGAAACGATTACAAAGCAAGAGGCGTTAATGAATATc                            >  1:4814650/1‑100 (MQ=255)                                                                 ccaacaCTACACAGTCAAATACAGAAAAGCAAATGACACCACAAGAAGCCGAAGATATAGTTCGAAACGATTACAAAGCAAGAGGCGTTAATGAATATCa                           >  1:5502163/2‑100 (MQ=255)                                                                         acacAGTCAAATACAGAAAAGCAAATGACACCACAAGAAGCCGAAGATATAGTTCGAAACGATTACAAAGCAAGAGGCGTTAATGAATATCAAACATTaa                   >  1:3459629/1‑100 (MQ=255)                                                                         acacAGTCAAATACAGAAAAGCAAATGACACCACAAGAAGCCGAAGATATAGTTCGAAACGATTACAAAGCAAGAGGCGTTAATGAATATCAAACATTaa                   >  1:2813428/1‑100 (MQ=255)                                                                          aacaGTCAAATACAGAAAAGCAAATGACACCACAAGAAGCCGAAGATATAGTTCGAAACGATTACAAAGCAAGAGGCGTTAATGAATATCAAACATTaaa                  >  1:4227090/2‑100 (MQ=255)                                                                            tataccaaTACAGAAAAGCAAATGACACCACAAGAAGCCGAAGATATAGTTCGAAACGATTACAAAGCAAGAGGCGTTAATGAATATCAAACATTAAAtt                >  1:405284/7‑100 (MQ=255)                                                                                cAAATACAGAAAAGCAAATGACACCACAAGAAGCCGAAGATATAGTTCGAAACGATTACAAAGCAAGGGGCGTTAATGAATATCAAACATTAAATTATaa            >  1:3531113/1‑100 (MQ=255)                                                                                      cAGAAAAGCAAATGACACCACAAGAAGCCGAAGATATAGTTCGAAACGATTACAAAGCAAGAGGCGTTAATGAATATCAAACATTAAATTATAAAACaaa      >  1:1859441/1‑100 (MQ=255)                                                                                          taaGCAAATGACACCACAAGAAGCCGAAGATATAGTTCGAAACGATTACAAAGCAAGAGGCGTTAATGAATATCAAACATTAAATTATAAAACAAATCtt  >  1:5122468/2‑100 (MQ=255)                                                                                              |                                                                                                GCTAATCAACATAAAGAAAATAGTAGTAAATCAAATGACACTAATAAAAAGACGCAACAAACTGACAACACTACACAGTCAAATACAGAAAAGTAAATGACACCACAAGAAGCCGAAGATATAGTTCGAAACGATTACAAAGCAAGAGGCGTTAATGAATATCAAACATTAAATTATAAAACAAATCTT  >  NC_007793/1924837‑1925025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑