Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	47,236	G→C	L95L (CTC→CTG)	SAUSA300_RS00200 ←	recombinase RecB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	47,236	0	G	C	100.0%	21.9 / NA	10	L95L (CTC→CTG)	SAUSA300_RS00200	recombinase RecB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (7/3);  total (7/3)

TGTAACATGAGTTTACCAGAAGAAGTATTGACTTCCATACGCTCTGACAAACTGAAAAATTCGACATTTTGTTTGTGTAAATCTTCGACAATTTTGAGAAGATCAGATGTATTACGAGCTAATCGGTTTGTTTTGTATACCATAACACAGTCGATATTGCCTTCTTTTGCATCT  >  NC_007793/47141‑47314                                                                                                |                                                                               tGTAACATGAGTTTACCAGAAGAAGTATTGACTTCCATACGCTCTGATAAACTGAAAAATTCGACATTTTGTTTATGCAAATCTTCGACGATTTTCagaa                                                                            >  1:2899233/1‑100 (MQ=255)  gTAACATGAGTTTACCAGAAGAAGTATTGACTTCCATACGCTCTGATAAACTGAAAAATTCGACATTTTGTTTATGCAAATCTTCGACGATTTTCagaag                                                                           >  1:5477560/1‑100 (MQ=255)             ctACCAGAAGAAGTATTGACTTCCATACGCTCTGATAAACTGAAAAATTCGACATTTTGTTTATGCAAATCTTCGACGATTTTCAGAAGATCCGATGTAt                                                                >  1:1868566/2‑100 (MQ=255)              tACCAGAAGAAGTATTGACTTCCATACGCTCTGATAAACTGAAAAATTCGACATTTTGTTTATGCAAATCTTCGACGATTTTCAGAAGATCCGATGTAtt                                                               >  1:2141676/1‑100 (MQ=255)               aCCAGAAGAAGTATTGACTTCCATACGCTCTGATAAACTGAAAAATTCGACATTTTGTTTATGCAAATCTTCGACGATTTTCAGAAGATCCGATGTATTa                                                              >  1:5598481/1‑100 (MQ=255)                       aagTATTGACTTCCATACGCTCTGATAAACTGAAAAATTCGACATTTTGTTTATGCAAATCTTCGACGATTTTCAGAAGATCCGATGTATTACGAGCTaa                                                      <  1:4713002/100‑1 (MQ=255)                                     gtACGCTCTGATAAACTGAAAAATTCGACATTTTGTTTATGCAAATCTTCGACGATTTTCAGAAGATCCGATGTATTACGAGCTAATCGGtttgttttgt                                        >  1:3864434/2‑100 (MQ=255)                                     aTACGCTCTGATAAACTGAAAAATTCGACATTTTGTTTATGCAAATCTTCGACGATTTTCAGAAGATCCGATGTATTACGAGCTAATCGGtttgtattgt                                        <  1:2524642/100‑1 (MQ=255)                                                                    tttgtttaTGCAAATCTTCGACGATTTTCAGAAGATCCGATGTATTACGAGCTAATCGGTTTGTTTTGTAGATCATAACACAATCTATATTGCCTTCttt         >  1:1696655/1‑100 (MQ=255)                                                                           atgcaaaTCTTCGACGATTTTCAGAAGATCCGATGTATTACGAGCTAATCGGTTTGTTTTGTAGATCATAACACAATCTATATTGCCTTCTTTTGCAtac  <  1:118557/96‑3 (MQ=255)                                                                                                |                                                                               TGTAACATGAGTTTACCAGAAGAAGTATTGACTTCCATACGCTCTGACAAACTGAAAAATTCGACATTTTGTTTGTGTAAATCTTCGACAATTTTGAGAAGATCAGATGTATTACGAGCTAATCGGTTTGTTTTGTATACCATAACACAGTCGATATTGCCTTCTTTTGCATCT  >  NC_007793/47141‑47314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑