Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,877,205	A→G	H228R (CAC→CGC)	SAUSA300_RS09290 →	NAD(FAD)‑utilizing dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,877,205	0	A	G	100.0%	52.1 / NA	21	H228R (CAC→CGC)	SAUSA300_RS09290	NAD(FAD)‑utilizing dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/7);  total (14/7)

CATCAGCTGAACCTTTCATCAAATCCAATCGTCTAAAAGGTTTAAGTTTAAAAGATGTTGAATTGTCAGTACTTAAGAAAAATGGTAAAAAACACATCAGTCATCAAATGGATATGTTATTTACTCATTTTGGTATCAGTGGTCCAGCTGCATTAAGATGTAGTCAGTTTGTTTATAAAGAACAAAAAAA  >  NC_007793/1877112‑1877301                                                                                              |                                                                                                 cATCAGCTGAACCTTTCATCAAATCCAATCGTCTAAAAGGTTTAAGTTTAAATGATGTTGAATTGTCAGTACTTAAGAAAAATGGCAAAAAACGCATCAg                                                                                            >  1:2238793/1‑100 (MQ=255)    cAGCTGAACCTTTCATCAAATCCAATCGTCTAAAAGGTTTAAGTTTAAAAGATGTTGAATTGTCAGTACTTAAGAAAAATGGTAAAAACCGCATCAgatc                                                                                         >  1:1137582/1‑97 (MQ=255)          aaCCTTTCATCAAATCCAATCGTCTAAAAGGTTTAAGTTTAAAAGATGTTGAATTGTCAGTACTTAAGAAAAATGGTAAAAAACGCATCAGTCATCAAAt                                                                                   <  1:2519870/100‑1 (MQ=255)                                   aaaaGGTTTAAGTTTAAAAGATGTTGAATTGTCAGTACTTAAGAAAAATGGTAAAAAACGCATCAGTCATCAAATGGATATGTTATTTACTCATTTTGGt                                                          >  1:4487038/1‑100 (MQ=255)                                   aaaaGGTTTAAGTTTAAAAGATGTTGAATTGTCAGTACTTAAGAAAAATGGTAAAAAACGCATCAGTCATCAAATGGATATGTTATTTACTCATTTTGGt                                                          >  1:2184984/1‑100 (MQ=255)                                            aagtttaaAAGATGTTGAATTGTCAGTACTTAAGAAAAATGGTAAAAAACGCATCAGTCATCAAATGGATATGTTATTTACTCATTTTGGTATCAGTGGt                                                 >  1:3471659/1‑100 (MQ=255)                                              gtttaaAAGATGTTGAATTGTCAGTACTTAAGAAAAATGGTAAAAAACGCATCAGTCATCAAATGGATATGCTATTTACTCATTTTGGTATCAGGGGTcc                                               <  1:5670691/100‑1 (MQ=255)                                                  aaaaGATGTTGAATTGTCAGTACTTAAGAAAAATGGTAAAAAACGCATCAGTCATCAAATGGATATGTTATTTACTCATTTTGGTATCAGTGGTCCAGca                                           <  1:3104200/100‑2 (MQ=255)                                                       aTGTTGAATTGTCAGTACTTAAGAAAAATGGTGAAAAACGCATCAGTCATCAAATGGATATGTTATTTACTCATTTTGGTATCGGTGGTCCAGCTGCAtt                                      <  1:3179573/100‑1 (MQ=255)                                                       aTGTTGAATTGTCAGTACTTAAGAAAAATGGTAAAAAACGCATCAGTCATCAAATGGATATGTTATTTACTCATTTTGGTATCAGTGGTCCAGCTGCAtt                                      <  1:2684323/100‑1 (MQ=255)                                                        tGTTGAATTGTCAGTACTTAAGAAAAATGGTAAAAAACGCATCAGTCATCAAATGGATATGTTATTTACTCATTTTGGTATCAGTGGTCCAGCTGCATTa                                     <  1:4427925/100‑1 (MQ=255)                                                        tGTTGAATTGTCAGTACTTAAGAAAAATGGTAAAAAACGCATCAGTCATCAAATGGATATGTTATTTACTCATTTTGGTATCAGTGGTCCAGCTGCATTa                                     <  1:1148506/100‑1 (MQ=255)                                                          ctGAATTGTCAGTACTTAAGAAAAATGGTAAAAAACGCATCAGTCATCAAATGGATATGTTATTTACTCATTTTGGTATCAGTGGTCCAGCTGCATTAAg                                   >  1:2283933/2‑100 (MQ=255)                                                                   cAGTACTTAAGAAAAATGGTAAAAAACGCATCAGTCATCAAATGGATATGTTATTTACTCATTTTGGTATCAGTGGTCCAGCTGCATTAAGATGTAGTCa                          >  1:363431/1‑100 (MQ=255)                                                                         ctAAGAAAAATGGTAAAAAACGCATCAGTCATCAAATGGATATGTTATTTACTCATTTTGGTATCAGTGGTCCAGCTGCATTAAGATGTAGTCAgtttgt                    >  1:406733/2‑100 (MQ=255)                                                                                  ctGGTAAAAAACGCATCAGTCATCAAATGGATATGTTATTTACTCATTTTGGTATCAGTGGTCCAGCTGCATTAAGATGTAGTCAGTTTGTTTATAAAGa           >  1:1880154/2‑100 (MQ=255)                                                                                    ggTAAAAAACGCATCAGTCATCAAATGGATATGTTATTTACTCATTTTGGTATCAGTGGTCCAGCTGCATTAAGATGTAGTCAGTTTGTTTATAAAGAAc         >  1:1831857/1‑100 (MQ=255)                                                                                    ggTAAAAAACGCATCAGTCATCAAATGGATATGTTATTTACTCATTTTGGTATCAGTGGTCCAGCTGCATTAAGATGTAGTCAGTTTGTTTATAAAGAAc         >  1:5352876/1‑100 (MQ=255)                                                                                           aaCGCATCAGTCATCAAATGGATATGTTATTTACTCATTTTGGTATCAGTGGTCCAGCTGCATTAAGATGTAGTCAGTTTGTTTATAAAGAACaaaaaaa  >  1:4078509/1‑100 (MQ=255)                                                                                           aaCGCATCAGTCATCAAATGGATATGTTATTTACTCATTTTGGTATCAGTGGTCCAGCTGCATTAAGATGTAGTCAGTTTGTTTATAAAGAACaaaaaaa  >  1:1404061/1‑100 (MQ=255)                                                                                           aaCGCATCAGTCATCAAATGGATATGTTATTTACTCATTTTGGTATCAGTGGTCCAGCTGCATTAAGATGTAGTCAGTTTGTTTATAAAGAACaaaaaaa  >  1:5261771/1‑100 (MQ=255)                                                                                              |                                                                                                 CATCAGCTGAACCTTTCATCAAATCCAATCGTCTAAAAGGTTTAAGTTTAAAAGATGTTGAATTGTCAGTACTTAAGAAAAATGGTAAAAAACACATCAGTCATCAAATGGATATGTTATTTACTCATTTTGGTATCAGTGGTCCAGCTGCATTAAGATGTAGTCAGTTTGTTTATAAAGAACAAAAAAA  >  NC_007793/1877112‑1877301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑