Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,862,563	T→C	E287E (GAA→GAG)	SAUSA300_RS09220 ←	cell division protein FtsK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,862,563	0	T	C	100.0%	214.3 / NA	75	E287E (GAA→GAG)	SAUSA300_RS09220	cell division protein FtsK
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/69);  total (6/69)

CTTGCTCTTCGTTTTTATGTTCTTCACGCTTTTGTTGCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCTTCAACAATTTGTGAAGCATAAATATTTTCAATTTTGATAGTATTATCAACTTTTGAATAATTAGGCATTGATTTTGTTGTTGATGATACATTTTC  >  NC_007793/1862466‑1862658                                                                                                  |                                                                                                cTTGCTCTTCGTTTTTATGTTCTTCACGCTTTTGTTGCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATttcctc                                                                                               <  1:728207/100‑1 (MQ=255) cTTGCTCTTCGTTTTTATGTTCTTCACGCTTTTGTTGCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATttcctc                                                                                               <  1:56365/100‑1 (MQ=255) cTTGCTCTTCGTTTTTATGTTCTTCACGCTTTTGTTGCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATttcctc                                                                                               <  1:5051834/100‑1 (MQ=255)  gatctctTCGTTTTTATGTTCTTCACGCTTTTGTTGCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATttcctcg                                                                                              <  1:2439885/97‑2 (MQ=255)   tGCTCTTCGTTTTTATGTTCTTCACGCTTTTGTTGCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTcaa                                                                                             >  1:733544/1‑100 (MQ=255)     ttccgatcttttATGTTCTTCACGCTTTTGTTGCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTcaaca                                                                                           <  1:5717075/92‑1 (MQ=255)     tcttccgatcttATGTCCTTCACGCTTTTGTTGCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTcaaca                                                                                           <  1:4368903/90‑1 (MQ=255)     ctctTCGTTTTTATGTTCTTCACGCTTTTGTTGCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTcatta                                                                                           <  1:1266276/100‑4 (MQ=255)     ctctTCGTTTTTATGTTCTTCACGCTTTTGTTGCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTcaaca                                                                                           <  1:3415503/100‑1 (MQ=255)       gttCGTTTTTATGTTCTTCACGCTTTTGTTGCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAAt                                                                                         <  1:2469104/99‑1 (MQ=255)        tccgatcttttGTTCTTCACGCTTTTGTTGCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAAtt                                                                                        <  1:3374303/90‑1 (MQ=255)         tCGTTTTTATGTTCTTCACGCTTTTGTTGCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAActt                                                                                       >  1:12360/1‑100 (MQ=255)         cgatctgtATGTTCTTCACGCTTTTGTTGCAACGCTTTTTTAAACCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAAttt                                                                                       <  1:2436228/93‑1 (MQ=255)          cGTTTTTATGTTCTTCACGCTTTTGTTGCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTtg                                                                                      <  1:975388/100‑1 (MQ=255)          cGTTTTTATGTTCTTCACGCTTTTGTTGCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTtg                                                                                      <  1:5440572/100‑1 (MQ=255)           ccgatcttGTTTTTCACGCTTTTGTTGCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTtgt                                                                                     <  1:4338117/93‑1 (MQ=255)           ccgatcttGTTCCTCACGCTTTTGTTGCAACGC‑TTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTtgtg                                                                                    <  1:5216354/93‑1 (MQ=255)            tttttATGTTCTTCACGCTTTTGTTGCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTtgtg                                                                                    <  1:2171681/100‑1 (MQ=255)            tctttATGTTCTTCACGCTTTTGTTGCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTtgtg                                                                                    <  1:1082246/98‑1 (MQ=255)            tctttATGTTCTTCACGCTTTTGTTGCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTtgtg                                                                                    <  1:2424340/98‑1 (MQ=255)            tctttATGCTCTTCACGCTTTTGTTGCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTtgtg                                                                                    <  1:969570/98‑1 (MQ=255)            cttttATGTTCTTCACGCTTTTGTTGCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTtgtg                                                                                    <  1:872042/99‑1 (MQ=255)            ctcttccgatcttcCCGCTTTTGTTGCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTtgtg                                                                                    <  1:1235764/91‑1 (MQ=255)            ctcttccgatctacACGCTTTTGTTGCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTtgta                                                                                    <  1:1643724/91‑2 (MQ=255)             ttttATGTTCTTCACGCTTTTGTTGCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGa                                                                                   <  1:3845838/100‑1 (MQ=255)             tcttccgatcttcGCGCTTTTGTTGCATCGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTtgtgt                                                                                   <  1:2823813/92‑2 (MQ=255)              tttATGTTCTTCACGCTTTTGTTGCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGaa                                                                                  >  1:720596/1‑100 (MQ=255)                tATGTTCTTCACGCTTTTGTTGCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGc                                                                                <  1:3330041/100‑1 (MQ=255)                  tGTTCTTCTCGCTTTTGTTGCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCAt                                                                              <  1:4997093/100‑1 (MQ=255)                  tGTTCTTCACGCTTTTGTTGCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCAt                                                                              <  1:1004835/100‑1 (MQ=255)                  tGTTCTTCACGCTTTTGTTGCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCAt                                                                              <  1:2519275/100‑1 (MQ=255)                  tGTTCTTCACGCTTTTGTTGCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCAt                                                                              <  1:5600283/100‑1 (MQ=255)                   gatcttcACGCTTTTGTTGCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCaag                                                                             <  1:5508122/98‑3 (MQ=255)                    ttcttcACGCTTTTGTTGCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCATat                                                                            <  1:4814258/100‑2 (MQ=255)                    ttcttcACGCTTTTGTTGCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCATag                                                                            <  1:409563/100‑2 (MQ=255)                         cACGCTTTTGTTGCAACGCTTTTTTAAATCGACGCTTTTGTAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCATAAATAtt                                                                       >  1:5534891/1‑100 (MQ=255)                         cACGCTTTTGTTGCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCATAAATAtt                                                                       >  1:2663210/1‑100 (MQ=255)                             cTTTTGTTGCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCATAAATATTTTCa                                                                   <  1:4740464/100‑1 (MQ=255)                              ttttGTTGCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCATAAATATTTTCaa                                                                  <  1:3561715/100‑1 (MQ=255)                               tttGTTGCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCATAAATATTTTCagt                                                                 <  1:2377316/100‑3 (MQ=255)                                     gCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCATAAATATTTTCAATTTTGAt                                                           <  1:5258743/100‑1 (MQ=255)                                     gCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCATAAATATTTTCAATTTTGAt                                                           <  1:2781297/100‑1 (MQ=255)                                     gCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCATAAATATTTTCAATTTTGAt                                                           <  1:3049078/100‑1 (MQ=255)                                       aaCGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCATAAATATTTTCAATTTTGATat                                                         <  1:5618859/100‑2 (MQ=255)                                        aCGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCATAAATATTTTCAATTTTGATAgg                                                        <  1:4077917/100‑2 (MQ=255)                                        aCGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCATAAATATTTTCAATTTTGATAGt                                                        <  1:1662626/100‑1 (MQ=255)                                        aCGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCATAAATATTTTCAATTCTGATAgg                                                        <  1:1959092/100‑2 (MQ=255)                                         cGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCATAAATATTTTCAATTTTGATAggc                                                       <  1:2549265/100‑3 (MQ=255)                                         cGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCATAAATATTTTCAATTTTGATAgga                                                       <  1:5071150/100‑3 (MQ=255)                                         cGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCATAAATATTTTCAATTTTGATAGta                                                       <  1:286066/100‑1 (MQ=255)                                           cTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCATAAATATTTTCAATTTTGATAGtatt                                                     <  1:5402265/100‑1 (MQ=255)                                           cTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCATAAATATTTTCAATTTTGATAGtatt                                                     <  1:3107060/100‑1 (MQ=255)                                             tttttAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCATAAATATTTTCAATTTTGATAGtattac                                                   <  1:5511331/100‑2 (MQ=255)                                                  aaaTCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCATAAATATTTTCAATTTTGATAGtatagtcctca                                              <  1:3934898/100‑9 (MQ=255)                                                                 aaGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCATAAATATTTTCAATTTTGATAGTATTATCAACTTTTGAATAATTAGGc                               <  1:2960884/100‑1 (MQ=255)                                                                 aaGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCATAAATATTTTCAATTTTGATAGTATTATCAACTTTTGAATAATTAGGc                               <  1:2930509/100‑1 (MQ=255)                                                                 aaGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCATAAATATTTTCAATTTTGATAGTATTATCAACTTTTGAATAATTAGGc                               <  1:892753/100‑1 (MQ=255)                                                                 aaGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCATAAATATTTTCAATTTTGATAGTATTATCAACTTTTGAATAATTAGGc                               <  1:5359569/100‑1 (MQ=255)                                                                 aaGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCATAAATATTTTCAATTTTGATAGTATTATCAACTTTTGAATAATTAGGc                               <  1:4522910/100‑1 (MQ=255)                                                                 aaGCACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCATAAATATTTTCAATTTTGATAGTATTATCAACTTTTGAATAATTAGGc                               <  1:4520186/100‑1 (MQ=255)                                                                  aGCACTTTACGTCCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCATAAATATTTTCAATTTTGATAGTATTATCAACTTTTGAATAATTAGGCa                              <  1:4422141/100‑1 (MQ=255)                                                                   gCACTTCACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCATAAATATTTTCAATTTTGATAGTATTATCAACTTTTGAATAATTAGGCAt                             <  1:3431089/100‑1 (MQ=255)                                                                    cACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCATAAATATTTTCAATTTTGATAGTATTATCAACTTTTGAATAATTAGGCAtt                            <  1:2935878/100‑1 (MQ=255)                                                                    cACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCATAAATATTTTCAATTTTGATAGTATTATCAACTTTTGAATAATTAGGCAtt                            <  1:2940925/100‑1 (MQ=255)                                                                    cACTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCATAAATATTTTCAATTTTGATAGTATTATCAACTTTTGAATAATTAGGCAtg                            <  1:3468802/100‑2 (MQ=255)                                                                     aCTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCATAAATATTTTCAATTTTGATAGTATTATCAACTTTTGAATAATTAGGCAtgt                           <  1:2910829/100‑3 (MQ=255)                                                                     aCTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCATAAATATTTTCAATTTTGATAGTATTATCAACTTTTGAATAATTAGGCATTg                           <  1:2944133/100‑1 (MQ=255)                                                                     aCTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCATAAATATTTTCAATTTTGATAGTATTATCAACTTTTGAATAATTAGGCATTg                           <  1:343350/100‑1 (MQ=255)                                                                     aCTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCATAAATATTTTCAATCTTGATAGTATTATCAACTTTTGAATAATTAGGCAtgt                           <  1:1832868/100‑3 (MQ=255)                                                                      cTTTACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCATAAATATTTTCAATTTTGATAGTATTATCAACTTTTGAATAATTAGGCAtttt                          <  1:4746457/100‑3 (MQ=255)                                                                         tACGTTCTCGTTCACGTCTAATTTCCTCAACAATTTGTGAAGCATAAACATTTTCAATTTTGATAGTATTATCAACTTTTGAATAATTAGGCATTGAttt                       <  1:3169193/100‑1 (MQ=255)                                                                                       cGTCTAATTTCCTCAACAATTTGTGAAGCATAAATATTTTCAATTTTGATAGTATTATCAACTTTTGAATAATTAGGCATTTACTTTGTTATTGataata         >  1:533697/1‑100 (MQ=255)                                                                                        gTCTAATTTCCTCAACAATTTGTGAAGCATAAATATTTTCAATTTTGATAGTATTATCAACTTTTGAATAATTAGGCATTGATTTTGTTGTTGATGATAc        <  1:5571154/100‑1 (MQ=255)                                                                                        gTCTAATTTCCTCAACAATTTGTGAAGCATAAATATTTTCAATTTTGATAGTATTATCAACTTTTGAATAATTAGGCATTGATTTTGTTGTTGATGATAc        <  1:886345/100‑1 (MQ=255)                                                                                        gTCTAATTTCCTCAACAATTTGTGAAGCATAAATATTTTCAATTTTGATAGTATTATCAACGTTTGAATAATTAGGCATTGATTTTGTTGTTGATGATAc        <  1:3516356/100‑1 (MQ=255)                                                                                          cTAATTTCCTCAACAATTTGTGAAGCATAAATATTTTCAATTTTGATAGTATTATCAACTTTTGAATAATTAGGCATTGATTTTGGTGTTGATGATACag      <  1:5519841/100‑2 (MQ=255)                                                                                           tAATTTCCTCAACAATTTGTGAAGCATAAATATTTTCAATTTTGATAGTATTATCAACTTTTGAATAATTAGGCATTGATTTTGTTGTTGATGATACAtt     <  1:4196669/100‑1 (MQ=255)                                                                                             aTTTCCTCAACAATTTGTGAAGCATAAATATTTTCAATTTTGATAGTATTATCAACTTTTGAATAATTAGGCATTGATTTTGTTGTTGATGATACAtttt   <  1:2884075/100‑1 (MQ=255)                                                                                              tttCCTCAACAATTTGTGAAGCATAAATATTTTCAATTTTGATAGTATTATCAACTTTTGAATAATTAGGCATTGATTTTGTTGTTGATGATACATTTtc  <  1:5653563/100‑1 (MQ=255)                                                                                                  |                                                                                                CTTGCTCTTCGTTTTTATGTTCTTCACGCTTTTGTTGCAACGCTTTTTTAAATCGACGCTTTTGAAGCACTTTACGTTCTCGTTCACGTCTAATTTCTTCAACAATTTGTGAAGCATAAATATTTTCAATTTTGATAGTATTATCAACTTTTGAATAATTAGGCATTGATTTTGTTGTTGATGATACATTTTC  >  NC_007793/1862466‑1862658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑