Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,851,021	C→T	intergenic (‑10/‑159)	SAUSA300_RS09170 ← / → SAUSA300_RS09175	acetate‑‑CoA ligase/acetoin dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,851,021	0	C	T	100.0%	46.3 / NA	17	intergenic (‑10/‑159)	SAUSA300_RS09170/SAUSA300_RS09175	acetate‑‑CoA ligase/acetoin dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (1/16);  total (1/16)

CCAATCAAAAGTATTATATGTTTCTTCATAATCTTTAAGGTTATGTTTACCTTGCGCTCCTTTATAAACTTCGACTTTCATTTGAAACTCCCCCTTTGTTATGTGAAAACGCTTTCTTTCTCAATATTATACATAACATTTCTAATTATTCAAAATTTTTACTTTTATCCTTTTTCT  >  NC_007793/1850931‑1851107                                                                                           |                                                                                       ctagtCAAAAGTATTATATGTTTCTTCATAATCTTTAAGGTTATGTTTACCTCGCGCTCCTTTATAAACTTCGACTTTCATTTGAAACTCTCCCTTTGtt                                                                               <  1:2859620/96‑1 (MQ=255) ccATTCAAAAGTATTATATGTTTCTTCATAATCTTTAAGGTTATGTTTACCTTGCGCTCCTTTATAAACTTCGACTTTCATTTGAAACTCTCCCTTTGtt                                                                               <  1:360456/100‑1 (MQ=255) ccAATCAAAAGTATTATATGTTTCTTCATAATCTTTAAGGTTATGTTTACCTTGCGCTCCTTTATAAACTTCGACTTTCATTTGAAACTCTCCCTTTGtt                                                                               <  1:5264086/100‑1 (MQ=255) ccAATCAAAAGTATTATATGTTTCTTCATAATCTTTAAGGTTATGTTTACCTTGCGCTCCTTTATAAACTTCGACTTTCATTTGAAACTCTCCCTTTGtt                                                                               <  1:2166418/100‑1 (MQ=255)  cAATCAAAAGTATTATATGTTTCTTCATAATCTTTAAGGTTATGTTTACCTTGCGCTCCTTTATAAACTTCGACTTTCATTTGAAACTCTCCCTTTGTTa                                                                              <  1:3991322/100‑1 (MQ=255)    ctCAAAAGTATTATATGTTTCTTCATAATCTTTAAGGTTATGTTTACCTTGCGCTCCTTTATAAACTTCGACTTTCATTTGAAACTCTCCCTTTGTTAtg                                                                            <  1:2563405/99‑1 (MQ=255)    aTCAAAAGTATTATATGTTTCTTCATAATCTTTAAGGTTATGTTTACCTTGCGCTCCTTTATAAACTTCGACTTTCATTTGAAACTCTCCCTTTGTTAtg                                                                            <  1:5097414/100‑1 (MQ=255)      tcttggTATTATATGTTTCTTCATAATCTTTAAGGTTATGTTTACCTTGCGCTCCTTTATAAACTTCGACTTTCATTTGAAACTCTCCCTTTGTTAtgtg                                                                          <  1:1350091/95‑1 (MQ=255)      ctaaaGTATTATATGTTTCTTCATAATCTTTAAGGTTATGTTTACCTTGCGCTCCTTTATAAACTTCGACTTTCATTTGAAACTCTCCCTTTGTTAtgtg                                                                          <  1:4596826/98‑1 (MQ=255)      cAAAAGTATTATATGTTTCTTCATAATCTTTAAGGTTATGTTTACCTTGCGCTCCTTTATAAACTTCGACTTTCATTTGAAACTCTCCCTTTGTTAtgtg                                                                          <  1:5631083/100‑1 (MQ=255)      cAAAAGTATTATATGTTTCTTCATAATCTTTAAGGTTATGTTTACCTTGCGCTCCTTTATAAACTTCGACTTTCATTTGAAACTCTCCCTTTGTTAtgtg                                                                          <  1:3915505/100‑1 (MQ=255)        aaaGTATTATATGTTTCTTCATAATCTTTAAGGTTATGTTTACCTTGCGCTCCTTTATAAACTTCGACTTTCATTTGAAACTCTCCCTTTGTTATGTGaa                                                                        <  1:3981460/100‑1 (MQ=255)        aaaGTATTATATGTTTCTTCATAATCTTTAAGGTTATGTTTACCTTGCGCTCCTTTATAAACTTCGACTTTCATTTGAAACTCTCCCTTTGTTATGTGaa                                                                        <  1:3461989/100‑1 (MQ=255)                   tGTTTCTTCATAATCTTTAAGGTTATGTTTACCTTGCGCTCCTTTATAAACTTCGACTTTCATTTGAAACTCTCCCTTTGTTATGTGAAAACGctttctt                                                             <  1:2059899/100‑1 (MQ=255)                                 cTTTAAGGTTATGTTTACCTTGCGCTCCTTTATAAACTTCGACTTTCATTCGAAACTCTCCCTTTGTTATGTGAAAACGCTTTCTTTCTCAATATTATAc                                               <  1:2626703/100‑1 (MQ=255)                                   ttAAGGTTATGTTTACCTTGCGCTCCTTTATAAACTTCGACTTTCATTTGAAACTCTCCCTTTGTTATGTGAAAACGCTTTCTTTCTCAATATTATACAt                                             >  1:1910477/1‑100 (MQ=255)                                                                              tCATTTGAAACTCTCCCTTTGTTATGTGAAAACGCTTTCTTTCTCAATATTATACATAACATTTCTAATTATTCAAAATTTTTACTTTTATCCTTTTTCt  <  1:5550184/100‑1 (MQ=255)                                                                                           |                                                                                       CCAATCAAAAGTATTATATGTTTCTTCATAATCTTTAAGGTTATGTTTACCTTGCGCTCCTTTATAAACTTCGACTTTCATTTGAAACTCCCCCTTTGTTATGTGAAAACGCTTTCTTTCTCAATATTATACATAACATTTCTAATTATTCAAAATTTTTACTTTTATCCTTTTTCT  >  NC_007793/1850931‑1851107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑