Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,846,346	A→T	R186R (CGT→CGA)	SAUSA300_RS09160 ←	iron‑regulated surface determinant protein H

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,846,346	0	A	T	100.0%	46.0 / NA	18	R186R (CGT→CGA)	SAUSA300_RS09160	iron‑regulated surface determinant protein H
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (0/18);  total (0/18)

AATTTAGTATAATCATAATTTGTTTCTTCTCCATCATCAATTTGAGTCGAAGAAACAATTTTCAATTCTTGTGTGCCATCTGAAACTGGGAAACGAATATAGGCATGGTCTTCTGGTACAGGACTATATGATACAAGTCTCACTGGCAATTTTTGATTGTTTTCATAGACTTCAAACTTCTTCCATGTTG  >  NC_007793/1846254‑1846443                                                                                             |                                                                                                  aaTTTAGTATAATCATAATTTGTTTCTTCTCCATCATCAATTTGAGTCGAAGAAACAATTTTCAATTCTTGTGTGCCATCTGAAACTGGGAATCGAatat                                                                                            <  1:1029895/100‑1 (MQ=255)     tAGTATAATCATAATTTGTTTCTTCTCCATCATCAATTTGAGTCGAAGAAACAATTTTCAATTCTTGTGTGCCATCTGAAACTGGGAATCGAATATAGGc                                                                                        <  1:2703567/100‑1 (MQ=255)       gTATAATCATAATTTGTTTCTTCTCCATCATCAATTTGAGTCGAAGAAACAATTTTCAATCCTTGTGTGCCATCTGAAACTGGGAATCGAATATAGGCag                                                                                      <  1:540468/100‑2 (MQ=255)        tataATCATAATTTGTTTCTTCTCCATCATCAATTTGAGTCGAAGAAACAATTTTCAATTCTTGTGTGCCATCTGAAACTGGGAATCGAATATAGGCATg                                                                                     <  1:2578181/100‑1 (MQ=255)          taATCATAATTTGTTTCTTCTCCATCATCAATTTGAGTCGAAGAAACAATTTTCAATTCTTGTGTGCCATCTGAAACTGGGAATCGAATATAGGCATGGt                                                                                   <  1:1148099/100‑1 (MQ=255)                 aaTTTGTTTCTTCTCCATCATCAATTTGAGTCGAAGAAACAATTTTCAATTCTTGTGTGCCATCTGAAACTGGGAATCGAATATAGGCATGGTCTTCTgg                                                                            <  1:2727904/100‑1 (MQ=255)                  aTTTGTTTCTTCTCCATCATCAATTTGAGTCGAAGAAACAATTTTCAATTCTTGTGTGCCATCTGAAACTGGGAATCGAATATAGGCATGGTCTTCTGGt                                                                           <  1:138778/100‑1 (MQ=255)                   tttGTTTCTTCTCCATCATCAATTTGAGTCGAAGAAACAATTTTCAATTCTTGTGTGCCATCTAAAACTGGGAATCGAATATAGGCATGGTCTTCTGGtg                                                                          <  1:487963/100‑2 (MQ=255)                    ttGTTTCTTCTCCATCATCAATTTGAGTCGAAGAAACAATTTTCAATTCTTGTGTGCCATCTGAAACTGGGAATCGAATATAGGCATGGTCTTCTGGTAc                                                                         <  1:5749922/100‑1 (MQ=255)                    ttGTTTCTTCTCCATCATCAATTTGAGTCGAAGAAACAATTTTCAATTCTTGTGTGCCATCTGAAACTGGGAATCGAATATAGGCATGGTCTTCTGGTAc                                                                         <  1:466662/100‑1 (MQ=255)                    ttGTTTCTTCTCCATCATCAATTTGAGTCGAAGAAACAATTTTCAATTCTTGTGTGCCATCTGAAACTGGGAATCGAATATAGGCATGGTCTTCTGGTAc                                                                         <  1:2839932/100‑1 (MQ=255)                    ttGTTTCTTCTCCATCATCAATTTGAGTCGAAGAAACAATTTTCAATTCTTGTGTGCCATCTGAAACTGGGAATCGAATATAGGCATGGTCTTCTGGTAc                                                                         <  1:1661153/100‑1 (MQ=255)                       tttCTTCTCCATCATCAATTTGAGTCGAAGAAACAATTTTCAATTCTTGTGTGCCATCTGAAACTGGGAATCGAATATAGGCATGGTCTTCTGGTACAgg                                                                      <  1:2959184/100‑1 (MQ=255)                                               tCGAAGAAACAATTTTCAATTCTTGTGTGCCATCTGAAACTGGGAATCGAATATAGGCATGGTCTTCTGGTACAGGACTATATGATACAAGTCTCACTgg                                              <  1:334874/100‑1 (MQ=255)                                                cGAAGAAACAATTTTCAATTCTTGTGTGCCATCTGAAACTGGGAATCGAATATAGGCATAGTCTTCTGGTACAGGACTATATGATACAAGTCTCACTGGc                                             <  1:2516032/100‑1 (MQ=255)                                                 gaagaaACAATTTTCAATTCTTGTGTGCCATCTGAAACTGGGAATCGAATATAGGCATGGTCTTCTGGTACAGGACTATATGATACAAGTCTCACTGGCa                                            <  1:2916776/100‑1 (MQ=255)                                                 gaagaaACAATTTTCAATTCTTGTGTGCCATCTGAAACTGGGAATCGAATATAGGCATGGTCTTCTGGTACAGGACTATATGATACAAGTCTCACTGGCa                                            <  1:481330/100‑1 (MQ=255)                                                                 aTTCTTGTGTGCCATCTGAAACTGGGAATCGAATATAGGCATGGTCTTCTGGTACAGGACTATATGATACAAGTCTCACTGGCAATTTTTGATTGTTTTc                            <  1:2688180/100‑1 (MQ=255)                                                                                           ggaCGAATATAGGCATGGTCTTCTGGTACAGGACTATATGATACAAGTCTCACTGGCAATTTTTGATTGTTTTCATAGACTTCAAACTTCTTCCATGTTg  <  1:3435934/98‑1 (MQ=255)                                                                                             |                                                                                                  AATTTAGTATAATCATAATTTGTTTCTTCTCCATCATCAATTTGAGTCGAAGAAACAATTTTCAATTCTTGTGTGCCATCTGAAACTGGGAAACGAATATAGGCATGGTCTTCTGGTACAGGACTATATGATACAAGTCTCACTGGCAATTTTTGATTGTTTTCATAGACTTCAAACTTCTTCCATGTTG  >  NC_007793/1846254‑1846443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑