Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,838,956	A→G	V75A (GTA→GCA)	SAUSA300_RS09130 ←	PTS glucose transporter subunit IIBC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,838,956	0	A	G	100.0%	175.8 / NA	66	V75A (GTA→GCA)	SAUSA300_RS09130	PTS glucose transporter subunit IIBC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (1/65);  total (1/65)

TATTTCCGAAGCTTTCATTCCTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTACACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTAAAATCGTAGTACCTACCGTAGTAAAGAAC  >  NC_007793/1838859‑1839051                                                                                                  |                                                                                                tatttCCGAAGCTTTCATTCCTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTgca                                                                                               <  1:3890881/100‑1 (MQ=255)  ctcttccgctcTTTCAATCCTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTgcac                                                                                              <  1:4789874/90‑1 (MQ=255)   tttCCGAAGCTTTCATTCCTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTGACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCAcc                                                                                             <  1:1995093/100‑1 (MQ=255)   tttCCGAAGCTTTCATTCCTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCAcc                                                                                             <  1:4740762/100‑1 (MQ=255)   tcttccgatcTTTCATTCCTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCAcc                                                                                             <  1:288545/91‑1 (MQ=255)     ttccgatcTTTCACTCCTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACcat                                                                                           <  1:4901244/93‑1 (MQ=255)     tCCGAAGCTTTCATTCCTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTgcacagg                                                                                           <  1:1053822/100‑4 (MQ=255)     tCCGAAGCTTTCATTCCTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACcag                                                                                           <  1:4159725/100‑2 (MQ=255)      tccgatcTTTCATTCCTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACcatc                                                                                          <  1:5161964/94‑1 (MQ=255)      ccGAAGCTTTCATTCCTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACcaag                                                                                          <  1:5037796/100‑3 (MQ=255)                   tCCTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGcc                                                                             <  1:3400220/100‑1 (MQ=255)                   tCCTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGcc                                                                             <  1:591505/100‑1 (MQ=255)                   tCCTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGcc                                                                             <  1:3231062/100‑1 (MQ=255)                     cTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCAt                                                                           <  1:3235743/100‑1 (MQ=255)                      taataaAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCAtt                                                                          <  1:3538673/100‑1 (MQ=255)                      taataaAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCAta                                                                          <  1:3135533/100‑2 (MQ=255)                      gatctgaGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCAtt                                                                          <  1:2979236/94‑1 (MQ=255)                        ataaAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGcccacac                                                                        <  1:338444/100‑6 (MQ=255)                           aaGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATTTCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCtat                                                                     <  1:4723267/100‑1 (MQ=255)                           aaGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCTCCATCATTTTTCTTTGCCATTCCtat                                                                     <  1:5647603/100‑1 (MQ=255)                           aaGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCtat                                                                     <  1:4377269/100‑1 (MQ=255)                           aaGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCtat                                                                     <  1:2003712/100‑1 (MQ=255)                           aaGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCtat                                                                     <  1:5600249/100‑1 (MQ=255)                           aaGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCtat                                                                     <  1:2700066/100‑1 (MQ=255)                           aaGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCtat                                                                     <  1:4943990/100‑1 (MQ=255)                           aaGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATCCCtat                                                                     <  1:344717/100‑1 (MQ=255)                            aGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCtatt                                                                    <  1:2216792/100‑2 (MQ=255)                            aGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCtata                                                                    <  1:2419493/100‑1 (MQ=255)                             ggCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAg                                                                   <  1:3497112/100‑1 (MQ=255)                               cgcTAATTTCATTGGTGATAGTACAACTGTAACTAAAAAATATCCTAACGTGGCCGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCt                                                                 <  1:4499353/100‑1 (MQ=255)                               cgcTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCt                                                                 <  1:4938379/100‑1 (MQ=255)                               cgcTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCt                                                                 <  1:3872433/100‑1 (MQ=255)                               cgcTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCt                                                                 <  1:4311967/100‑1 (MQ=255)                               cgcTAATTTCATTGGTGATAACACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGca                                                                 <  1:3932134/100‑2 (MQ=255)                                gcTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTa                                                                <  1:4354908/100‑1 (MQ=255)                                gcTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTCTCTTTGCCATTCCTATAGCTa                                                                <  1:1074021/100‑1 (MQ=255)                                 cTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTac                                                               <  1:630881/100‑1 (MQ=255)                                 cTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTac                                                               <  1:4856833/100‑1 (MQ=255)                                   aaTTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTagat                                                             <  1:1232485/100‑4 (MQ=255)                                     tttCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCa                                                           <  1:4990328/100‑1 (MQ=255)                                       tCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTacatgtag                                                         <  1:274832/100‑6 (MQ=255)                                       tCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAAt                                                         <  1:3207112/100‑1 (MQ=255)                                          ttGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGc                                                      <  1:2794464/100‑1 (MQ=255)                                          ttGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGc                                                      <  1:4542394/100‑1 (MQ=255)                                                                tAAAAAATATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGctct                                <  1:4146783/100‑1 (MQ=255)                                                                 aaaaaaTATCCTAACTTAGCTGCAAGCGCGACTGCACCATCATATTTCTTTGCCATACCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTa                               <  1:933918/100‑1 (MQ=255)                                                                 aaaaaaTATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTa                               <  1:4052106/100‑1 (MQ=255)                                                                 aaaaaaTATCCTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTa                               <  1:3025503/100‑1 (MQ=255)                                                                           cTAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTAAAATCgtaca                     <  1:1648745/100‑3 (MQ=255)                                                                            tAACGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTAAAATCgtagta                    <  1:2516476/100‑1 (MQ=255)                                                                             aaCGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAACTAAAATACCTAATTGCTCTAAAATCGTAGtac                   >  1:1774499/1‑100 (MQ=255)                                                                              aCGTAGCTGCAAGCGCGACTGCACCAACATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATAGCTCTAAAATCGTAGtacc                  <  1:737028/100‑1 (MQ=255)                                                                               cGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTAAAATCGTAGtacct                 <  1:5455114/100‑1 (MQ=255)                                                                               cGTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTAAAATCGTAGtacct                 <  1:1889642/100‑1 (MQ=255)                                                                               cGTAGCCGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTAAAATCGTAGtacct                 <  1:3845231/100‑1 (MQ=255)                                                                                gTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTAAAATCGTAGtacctt                <  1:5017089/100‑2 (MQ=255)                                                                                gTAGCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTAAAATCGTAGtaccta                <  1:3638447/100‑1 (MQ=255)                                                                                   gCTGCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTAAAATCGTAGTACCTACCg             <  1:4520652/100‑1 (MQ=255)                                                                                      gCAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTAAAATCGTAGTACCTACCgtag          <  1:2984808/100‑1 (MQ=255)                                                                                       cAAGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTAAAATCGTAGTACCTACCgtagt         <  1:1954390/100‑1 (MQ=255)                                                                                        aaGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTAAAATCGTAGTACCTACCgtagta        <  1:212517/100‑1 (MQ=255)                                                                                        aaGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTAAAATCGTAGTACCTACCgtagta        <  1:2316356/100‑1 (MQ=255)                                                                                        aaGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTAAAATCGTAGTACCTACCgtagta        <  1:2659322/100‑1 (MQ=255)                                                                                        aaGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTAAAATCGTAGTACCTACCgtagta        <  1:5795648/100‑1 (MQ=255)                                                                                         aGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTAAAATCGTAGTACCTACCGTAGTaa       <  1:4504936/100‑1 (MQ=255)                                                                                         aGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTAAAATCGTAGTACCTACCGTAGTaa       <  1:42059/100‑1 (MQ=255)                                                                                         aGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTAAAATCGTAGTACCTACCGTAGTaa       <  1:5255747/100‑1 (MQ=255)                                                                                         aGCGCGACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTAAAATCGTAGTACCTACCGCAGTaa       <  1:3865310/100‑1 (MQ=255)                                                                                              gACTGCACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATGAAATACCTAATAGCTCTAAAATCGTAGTGCCTACCGTAGTAAAGAAc  <  1:4865953/100‑1 (MQ=255)                                                                                                  |                                                                                                TATTTCCGAAGCTTTCATTCCTAATAAAGGCGCTAATTTCATTGGTGATAATACAACTGTAACTAAAAAATATCCTAACGTAGCTGCAAGCGCGACTACACCATCATTTTTCTTTGCCATTCCTATAGCTACACCAATTGCAAATAAAATACCTAATTGCTCTAAAATCGTAGTACCTACCGTAGTAAAGAAC  >  NC_007793/1838859‑1839051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑