Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,798,849	A→G	D237D (GAT→GAC)	SAUSA300_RS08950 ←	isocitrate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,798,849	0	A	G	100.0%	179.5 / NA	67	D237D (GAT→GAC)	SAUSA300_RS08950	isocitrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (1/66);  total (1/66)

TGAGCAGCATTAGCAGCATCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAATCGTAACCCCACTGCTTAAATGAGCCTTCTGTAAATTTCATAATATTACCTTTATGAACTAAAGTAACTG  >  NC_007793/1798754‑1798919                                                                                                |                                                                       tGAGCAGCATTAGCAGCATCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGt                                                                    <  1:3761136/100‑1 (MQ=255)  gAGCAGCATTAGCAGCATCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTa                                                                   <  1:2593643/100‑1 (MQ=255)  gAGCAGCATTAGCAGCATCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTa                                                                   <  1:4454216/100‑1 (MQ=255)       gcaTTAGCAGCATCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAAcccc                                                              <  1:753720/100‑1 (MQ=255)        tgctcttccgaTCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCa                                                             <  1:2827919/90‑1 (MQ=255)         aTTAGCAGCATCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCat                                                            <  1:1341775/100‑2 (MQ=255)         aTTAGCAGCATCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCAc                                                            <  1:1088625/100‑1 (MQ=255)         aTTAGCAGCATCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCAc                                                            <  1:1552428/100‑1 (MQ=255)          ttAGCAGCATCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCaag                                                           <  1:1551766/100‑3 (MQ=255)          ttAGCAGCATCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCAca                                                           <  1:2057764/100‑2 (MQ=255)          ttAGCAGCATCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACt                                                           <  1:1520322/100‑1 (MQ=255)          ttAGCAGCATCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACt                                                           <  1:2103777/100‑1 (MQ=255)          ttAGCAGCATCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACt                                                           <  1:1532050/100‑1 (MQ=255)          ttAGCAGCATCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACt                                                           <  1:4799559/100‑1 (MQ=255)          ttAGCAGCATCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACt                                                           <  1:5663886/100‑1 (MQ=255)          ttAGCAGCATCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACt                                                           <  1:957842/100‑1 (MQ=255)           tcttccgaTCTCTGCCCTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTg                                                          <  1:1443437/93‑1 (MQ=255)           tAGCAGCATCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTg                                                          <  1:1450880/100‑1 (MQ=255)           tAGCAGCATCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTg                                                          <  1:162785/100‑1 (MQ=255)           tAGCAGCATCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTg                                                          <  1:3022270/100‑1 (MQ=255)           tAGCAGCATCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTg                                                          <  1:1186954/100‑1 (MQ=255)           tAGCAGCATCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTg                                                          <  1:2107997/100‑1 (MQ=255)            agcagcATCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTga                                                         <  1:1087645/100‑2 (MQ=255)            agcagcATCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGc                                                         <  1:2349382/100‑1 (MQ=255)            agcagcATCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGc                                                         <  1:2950605/100‑1 (MQ=255)             gcagcaTCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTgag                                                        <  1:4387791/100‑3 (MQ=255)             gcagcaTCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGca                                                        <  1:818885/100‑2 (MQ=255)              cggcaTCTCTGGCTTCATTTTCAACAATGTCGGCATATTGTTGCCAAGAGAATACTTGATCACCTGATTCAGATAATGCGAAGTCGTAACCCCTCTGCtt                                                       <  1:2871982/98‑1 (MQ=255)              cagcaTCTCTTCCTTCATTTTCAACCATTTCGTCATATTGTTGCCAAGTGAATACTTGATCTCCAAATTCAGATAATGCTAAGTCGTAACCCCACTGCtt                                                       <  1:4073808/100‑1 (MQ=255)              cagcaTCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGCtt                                                       <  1:4453114/100‑1 (MQ=255)              cagcaTCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGCtt                                                       <  1:4105844/100‑1 (MQ=255)              cagcaTCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGCtt                                                       <  1:4750450/100‑1 (MQ=255)              cagcaTCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGCtt                                                       <  1:1179080/100‑1 (MQ=255)              cagcaTCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGCtt                                                       <  1:5014656/100‑1 (MQ=255)              cagcaTCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGCtt                                                       <  1:5161973/100‑1 (MQ=255)              cagcaTCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGCtt                                                       <  1:5266859/100‑1 (MQ=255)              cagcaTCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGCtt                                                       <  1:5461628/100‑1 (MQ=255)              cagcaTCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGCtt                                                       <  1:22055/100‑1 (MQ=255)              cagcaTCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGCtt                                                       <  1:662418/100‑1 (MQ=255)              cagcaTCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGCtt                                                       <  1:2250448/100‑1 (MQ=255)               ccgaTCTCTGCCCTCATTCTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGCttg                                                      <  1:5770808/97‑2 (MQ=255)               agcaTCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGCTTa                                                      <  1:3501682/100‑1 (MQ=255)               agcaTCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGCTTa                                                      <  1:3269348/100‑1 (MQ=255)               agcaTCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGCTTa                                                      <  1:4284475/100‑1 (MQ=255)                tccgatctGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGCTTaa                                                     <  1:2395379/95‑1 (MQ=255)                gcaTCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGCTTag                                                     <  1:3497822/100‑2 (MQ=255)                 caTCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGCttgct                                                    <  1:4599221/100‑4 (MQ=255)                 caTCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGCTTaag                                                    <  1:5488997/100‑2 (MQ=255)                   tctcTGCTTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGCTTAAATg                                                  <  1:2050639/100‑1 (MQ=255)                   tctcTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGCTTAAATg                                                  <  1:2396336/100‑1 (MQ=255)                   tctcTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGCTTAAATg                                                  <  1:470484/100‑1 (MQ=255)                   tctcTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGCTTAAATg                                                  <  1:2647650/100‑1 (MQ=255)                   tctcTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGCTTAAATg                                                  <  1:1176348/100‑1 (MQ=255)                   tctcTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGCTTAAATg                                                  <  1:3388428/100‑1 (MQ=255)                   tctcTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAACACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGCTTAAATg                                                  <  1:285873/100‑1 (MQ=255)                     tctGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATGCTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGCTTAAATGAg                                                <  1:4412609/100‑1 (MQ=255)                      ctGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGCTTAAATGAGc                                               <  1:5433325/100‑1 (MQ=255)                         ccTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGCTTAAATGAGCCtt                                            <  1:1506862/100‑1 (MQ=255)                         ccTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGCTTAAATGAGCCtt                                            <  1:3602198/100‑1 (MQ=255)                             cATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGCTTAAATGAGCCTTCTgc                                        <  1:1126318/100‑2 (MQ=255)                             cATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGCTTAAATGAGCCTTCTGt                                        <  1:5301921/100‑1 (MQ=255)                             cATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGCTTAAATGAGCCTTCTGt                                        <  1:3724133/100‑1 (MQ=255)                              aTTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGCTTAAATGAGCCTTCTGTa                                       <  1:4547723/100‑1 (MQ=255)                                       aaTTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGCTTAAATGAGCCTTCTGTAAATTTCata                              <  1:5135303/100‑1 (MQ=255)                                                 tatTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGCTTAAATGAGCCTTCTGTAAATTTCATAATATTACCtt                    <  1:4886853/100‑1 (MQ=255)                                                                attACTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGCTTAAATGAGCCTTCTGTAAATTTCATAATATTACCTTTATGAACTAAAGTaa     >  1:1058239/3‑100 (MQ=255)                                                                   aCTTGATCACCAAATTCAGATAATGCTAAGTCGTAACCCCACTGCTTAAATGAGCCTTCTGTAAATTTCATAATATTACCTTTATGAACTAAAGTAACTg  <  1:169654/100‑1 (MQ=255)                                                                                                |                                                                       TGAGCAGCATTAGCAGCATCTCTGCCTTCATTTTCAACAATTTCGTCATATTGTTGCCAAGTGAATACTTGATCACCAAATTCAGATAATGCTAAATCGTAACCCCACTGCTTAAATGAGCCTTCTGTAAATTTCATAATATTACCTTTATGAACTAAAGTAACTG  >  NC_007793/1798754‑1798919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑