| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_007793 | 1,712,124 | T→C | E238G (GAA→GGA) | SAUSA300_RS08520 ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_007793 | 1,712,124 | 0 | T | C | 94.4% | 39.8 / ‑4.3 | 18 | E238G (GAA→GGA) | SAUSA300_RS08520 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (12/5); total (13/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.90e-01 | |||||||||||
GTCACTTTCTTTTTTAAGCTTTAAATTTCTCCCCATTTTTTTAGCCCCCTATAAGGATTGAATATCAATGCCTTCTTTCATTAAAATTTCTCTAATTTTCGAAACAAATAATAATGCATGTTCTCCATCACCATGCACACAAATTGTATCTGCTTGTAACGTTACTTCCTTATTGTTTTTT > NC_007793/1712036‑1712216 | gTCACTTTCTTTTTTAAGCTTTAAATTTCTCCCCATTTTTTTAGCCCCCTATAAGGATTGAATATCAATGCCTTCTTTCATTAAAATTTCTCTAATTTTc > 1:4205103/1‑100 (MQ=255) gCTTTAAATTTCTCCCCATTTTTTTAGCCCCCTATAAGGATTGAATATCAATGCCTTCTTTCATTAAAATTCCTCTAATTTTCGAAACAAATAATAATGc < 1:2108818/100‑1 (MQ=255) ccccATTTTTTTAGCCCCCTATAAGGATTGAATATCAATGCCTTCTTTCATTAAAATTCCTCTAATTTTCGAAACAAATAATAATGCATGTTCTCCATCa > 1:1619218/1‑100 (MQ=255) cccATTTTTTTAGCCCCCTATAAGGATTGAATATCAATGCCTTCTTTCATTAAAATTCCTCTAATTTTCGAAACAAATAATAATGCATGTTCTCCATCAc > 1:3949963/1‑100 (MQ=255) ctttttttttAGCCCCCTATAAGGATTGAATATCAATGCCTTCTTTCATTAAAATTCCTCTAATTTTCGAAACAAATAATAATGCATGTTCTCCATCAcc < 1:4316803/97‑1 (MQ=255) cttccgatctgCCCCCTATAAGGATTGAATATCAATGCCTTCTTTCATTAAAATTCCTCTAATTTTCGAAACAAATAATAATGCATGTTCTCCATCACCa < 1:4585113/90‑1 (MQ=255) tctgCCCCCTATAAGGATTGAATATCAATGCCTTCTTTCATTAAAATTCCTCTAATTTTCGAAACAAATAATAATGCATGTTCTCCATCACCATGcacag < 1:939984/97‑2 (MQ=255) tAGCCCCCTATAAGGATTGAATATCAATGCCTTCTTTCATTAAAATTCCTCTAATTTTCGAAACAAATAATAATGCATGTTCTCCATCACCATGcacaca > 1:1239966/1‑100 (MQ=255) tAGCCCCCTATAAGGATTGAATATCAATGCCTTCTTTCATTAAAATTCCTCTAATTTTCGAAACAAATAATAATGCATGTTCTCCATCACCATGcacaca > 1:5109301/1‑100 (MQ=255) tAGCCCCCTATAAGGATTGAATATCAATGCCTTCTTTCATTAAAATTCCTCTAATTTTCGAAACAAATAATAATGCATGTTCTCCATCACCATGcacaca > 1:4853737/1‑100 (MQ=255) tAGCCCCCTATAAGGATTGAATATCAATGCCTTCTTTCATTAAAATTCCTCTAATTTTCGAAACAAATAATAATGCATGTTCTCCATCACCATGcacaca > 1:4413548/1‑100 (MQ=255) aGCCCCCTATAAGGATTGAATATCAATGCCTTCTTTCATTAAAATTCCTCTAATTTTCGAAACAAATAATAATGCATGTTCTCCATCACCaagatcggaa > 1:1190378/1‑91 (MQ=255) gCCCCCTATAAGGATTGAATATCAATGCCTTCTTTCATTAAAATTCCTCTAATTTTCGAAACAAATAATAATGCATGTTCTCCATCACCATGCACACaaa > 1:3290822/1‑100 (MQ=255) acccccTATAAGGATTGAATATCAATGCCTTCTTTCATTAAAATTCCTCTAATTTTCGAAACAAATAATAATGCATGTTCTCCATCACCATGCACACaaa > 1:7557/2‑100 (MQ=255) cccccTATAAGGATTGAATATCAATGCCTTCTTTCATTAAAATTCCTCTAATTTTCGAAACAAATAATAATGCATGTTCTCCATCACCATGCACACAAAt > 1:4249263/1‑100 (MQ=255) cAATGCCTTCTTTCATTAAAATTCCTCTAATTTTCGAAACAAATAATAATGCATGTTCTCCATCACCATGCACACAAATTGTATCTGCTTGTAACGTTAc > 1:1358535/1‑100 (MQ=255) cAATGCCTTCTTTCATTAAAATTCCTCTAATTTTCGAAACAAATAATAATGCATGTTCTCCATCACCATGCACACAAATTGTATCTGCTTGTAACGTTAc > 1:631765/1‑100 (MQ=255) tAAAATTCCTCTAATTTTCGAAACAAATAATAATGCATGTTCTCCATCACCATGCACACAAATTGTATCTGCTTGTAACGTTACTTCCTTATTGtttttt < 1:3445545/100‑1 (MQ=255) | GTCACTTTCTTTTTTAAGCTTTAAATTTCTCCCCATTTTTTTAGCCCCCTATAAGGATTGAATATCAATGCCTTCTTTCATTAAAATTTCTCTAATTTTCGAAACAAATAATAATGCATGTTCTCCATCACCATGCACACAAATTGTATCTGCTTGTAACGTTACTTCCTTATTGTTTTTT > NC_007793/1712036‑1712216 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |