Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,699,824	A→C	V203G (GTT→GGT)	SAUSA300_RS08435 ←	competence protein ComEC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,699,824	0	A	C	100.0%	16.0 / NA	8	V203G (GTT→GGT)	SAUSA300_RS08435	competence protein ComEC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (3/5);  total (3/5)

GACAAATAAAGGTAAATTTAATCGTTTTAAAGGTTGGTAAATTAAAAATACAATTGCAGCTATATGCGAGCCACTAACTGCCAGCAAATGATATATAACTATCTCTTTAACACGTTCCTTAAATTGCTCATTAACTTCTTTTACGTCACCAGTAATCAATGCCATAATACGATCCGGAAACTTAATACC  >  NC_007793/1699727‑1699915                                                                                                  |                                                                                            gACAAATAAAGGTAAATTTAATCGTTTTAAAGGTTGGTAAATTAAAAATACAATTGCAGCTATATGCGAGCCACTAACTGCCAGCAAATGATATATACCt                                                                                           >  1:3606386/1‑100 (MQ=255)                      tCGTTTTAAAGGTTGGTAAATTAAAAATACAATTGCAGCTATATGCGAGCCACTAACTGCCAGCAAATGATATATACCTATCTCTTTAACACGTTCCTTa                                                                      <  1:4270083/100‑1 (MQ=255)                             aaaGGTTGGTAAATTAAAAATACAATTGCAGCTATATGCGAGCCACTAACTGCCAGCAAATGATATATACCTATCTCTTTAACACGTTCCTTAAATTGCt                                                               >  1:5250977/1‑100 (MQ=255)                             aaaGGTTGGTAAATTAAAAATACAATTGCAGCTAGATGCGAGTCACTAACTGCCAGCAAATGATATATACCTATCTCTTTAACACGTTCCTTAAATTGCt                                                               <  1:2771159/100‑1 (MQ=255)                                                                  gCGAGCCACTAACTGCCAGCAAATGATATATACCTATCTCTTTAACACGTTCCTTAAATTGCTCATTAACTTCTTTTACGTCACCAGTAATCAATGCCat                          <  1:3806797/100‑1 (MQ=255)                                                                    ctcttccgatcTGCCAGCAAATGATATATACCTATCTCTTTAACACGTTCCTTAAATTGCTCATTAACTTCTTTTACGTCACCAGTAATCAATGCCataa                        <  1:3402468/90‑1 (MQ=255)                                                                      tccACTAACTGCCAGCAAATGATATATACCTATCTCTTTAACACGTTCCTTAAATTGCTCATTAACTTCTTTTACGTCACCAGTAATCAATGCCataata                      >  1:2974716/2‑100 (MQ=255)                                                                                          gATATATACCTATCTCTTTAACACGTTCCTTAAATTGCTCATTAACTTCTTTTACGTCACCAGTAATCAATGCCATAATACGATCCGGAAACTTAATAcc  <  1:2345849/100‑1 (MQ=255)                                                                                                  |                                                                                            GACAAATAAAGGTAAATTTAATCGTTTTAAAGGTTGGTAAATTAAAAATACAATTGCAGCTATATGCGAGCCACTAACTGCCAGCAAATGATATATAACTATCTCTTTAACACGTTCCTTAAATTGCTCATTAACTTCTTTTACGTCACCAGTAATCAATGCCATAATACGATCCGGAAACTTAATACC  >  NC_007793/1699727‑1699915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑