Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,693,739	T→C	T145A (ACA→GCA)	SAUSA300_RS08410 ←	coproporphyrinogen III oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,693,739	0	T	C	100.0%	16.2 / NA	8	T145A (ACA→GCA)	SAUSA300_RS08410	coproporphyrinogen III oxidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/8);  total (0/8)

CCATATCTAAAGCTAGATCTAAACTTTGTTCAAAATCTTCAATCGTCTGTTTCGGTAAATGATACATTAAATCTAAACTGATTGATTTAATACCTGTGTTTTTAGCATTTAACACCGAAGTGTAAATATCTTCAGTATTGTGCGTTCTACCTAAAACAGACAATAACTCCGGCTTGA  >  NC_007793/1693643‑1693819                                                                                                 |                                                                                 ccATATCTAAAGCTAGATCTAAACTTTGTTCAAAATCTTCAATCGTCTGTTTCGGTAAATGATACATTAAATCTAAACTGATTGATTTAATACCTGCGtt                                                                               <  1:3920626/100‑1 (MQ=255)                                                  tttCGGTAAATGATACATTAAATCTAAACTGATTGATTTAATACCTGCGTTTTTAGCATTTAACACCGAAGTGTAAATATCTTCAGTATTGTGCGTTCTa                              <  1:5107094/100‑1 (MQ=255)                                                     cGGTAAATGATACATTAAATCTAAACTGATTGATTTAATACCTGCGTTTTTAGCATTTAACACCGAAGTGTAAATATCTTCAGTATTGTGCGTTCTACCt                           <  1:2057199/100‑1 (MQ=255)                                                      ggTAAATGATACATTAAATCTAAACTGATTGATTTAATACCTGCGTTTTTAGCATTTAACACCGAAGTGTAAATATCTTCAGTATTGTGCGTTCTACCTa                          <  1:1747147/100‑1 (MQ=255)                                                      ggTAAATGATACATTAAATCTAAACTGATTGATTTAATACCTGCGTTTTTAGCATTTAACACCGAAGTGTAAATATCTTCAGTATTGTGCGTTCTACCTa                          <  1:634975/100‑1 (MQ=255)                                                                           aaaCTGATTGATTTAATACCTGCGTTTTTAGCATTTAACACCGAAGTGTAAATATCTTCAGTATTGTGCGTTCTACCTAAAACAGACAATAACTCCGGCt     <  1:3314823/100‑1 (MQ=255)                                                                           aaaCTGATTGATTTAATACCTGCGTTTTTAGCATTTAACACCGAAGTGTAAATATCTTCAGTATTGTGCGTTCTACCTAAAACAGACAATAACTCCGGCt     <  1:5329597/100‑1 (MQ=255)                                                                              cTGATTGATTTAATACCTGCGTTTTTAGCATTTAACACCGAAGTGTAAATATCTTCAGTATTGTGCGTTCTACCTAAAACAGACAATAACTCCGGCTTGa  <  1:1755361/100‑1 (MQ=255)                                                                                                 |                                                                                 CCATATCTAAAGCTAGATCTAAACTTTGTTCAAAATCTTCAATCGTCTGTTTCGGTAAATGATACATTAAATCTAAACTGATTGATTTAATACCTGTGTTTTTAGCATTTAACACCGAAGTGTAAATATCTTCAGTATTGTGCGTTCTACCTAAAACAGACAATAACTCCGGCTTGA  >  NC_007793/1693643‑1693819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑