Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,663,728	T→C	I30V (ATA→GTA)	SAUSA300_RS08255 ←	penicillin‑binding protein 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,663,728	0	T	C	100.0%	167.4 / NA	59	I30V (ATA→GTA)	SAUSA300_RS08255	penicillin‑binding protein 3
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/59);  total (0/59)

TAATGTTTTCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATATAATCACACCAAATATAAAGTTAATTCTCTTGTTAATTGTATTTTGAACGATTTCATCATTTGATTTTTCTTTTAGTCTTTTTAACAAAACTACCT  >  NC_007793/1663632‑1663823                                                                                                 |                                                                                                tAATGTTTTCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAAt                                                                                              <  1:3589148/100‑1 (MQ=255)  aaTGTTTTCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTGCTGCAAAAATAAATACAATc                                                                                             <  1:2984112/100‑1 (MQ=255)  aaTGTTTTCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAAtt                                                                                             <  1:2513730/100‑2 (MQ=255)  aaTGTTTTCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATc                                                                                             <  1:5294366/100‑1 (MQ=255)  aaTGTTTTCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATc                                                                                             <  1:1964079/100‑1 (MQ=255)    tGTTTTCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATcac                                                                                           <  1:1136774/100‑1 (MQ=255)    tGTTTTCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATcac                                                                                           <  1:206069/100‑1 (MQ=255)    tGTTTTCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATcac                                                                                           <  1:4997102/100‑1 (MQ=255)    tGTTTTCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATcac                                                                                           <  1:3662470/100‑1 (MQ=255)     gTTTTCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATcacc                                                                                          <  1:1340403/100‑2 (MQ=255)     gTTTTCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATcaca                                                                                          <  1:1437711/100‑1 (MQ=255)      ttttCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATcacat                                                                                         <  1:4370662/100‑2 (MQ=255)      ttttCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATcacag                                                                                         <  1:3612406/100‑2 (MQ=255)       tttCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACTTAGTACTAGTACTGCAAAAATAAATACAATCACAcc                                                                                        <  1:1829985/100‑1 (MQ=255)       tttCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATcacata                                                                                        <  1:587094/100‑3 (MQ=255)       tttCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACAcc                                                                                        <  1:2908690/100‑1 (MQ=255)       tttCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACAcc                                                                                        <  1:1579933/100‑1 (MQ=255)        ttCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCa                                                                                       <  1:3638729/100‑1 (MQ=255)        ttCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCa                                                                                       <  1:5568068/100‑1 (MQ=255)        ttCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCa                                                                                       <  1:2952277/100‑1 (MQ=255)        ttCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCa                                                                                       <  1:821035/100‑1 (MQ=255)        ttCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCa                                                                                       <  1:1067151/100‑1 (MQ=255)        ttCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCa                                                                                       <  1:1845680/100‑1 (MQ=255)        ttCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCa                                                                                       <  1:1807377/100‑1 (MQ=255)        ttCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCa                                                                                       <  1:1324719/100‑1 (MQ=255)        ttCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAACCACACCa                                                                                       <  1:3429046/100‑1 (MQ=255)        ttCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGC‑AAAATAAATACAATCACACCaa                                                                                      <  1:5158632/100‑1 (MQ=255)         tcatcaTTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCag                                                                                      <  1:5186101/100‑2 (MQ=255)         tcatcaTTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCaa                                                                                      <  1:5283411/100‑1 (MQ=255)         tcatcaTTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCaa                                                                                      <  1:4918967/100‑1 (MQ=255)          catcatTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCaag                                                                                     <  1:1353407/100‑2 (MQ=255)           atcatTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCaacg                                                                                    <  1:1993661/100‑3 (MQ=255)             catTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCAAatat                                                                                  <  1:5373211/100‑1 (MQ=255)               ttttttATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCAAATATaa                                                                                <  1:5293915/100‑1 (MQ=255)                tttttATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAGCGTAGTACTAGTACTGCAAAAATAAATACAATCACACCAAATATaac                                                                               <  1:4289215/100‑2 (MQ=255)                 ttttATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCAAATATAAAg                                                                              <  1:311501/100‑1 (MQ=255)                   ttATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCAAATATAAAGtt                                                                            <  1:413527/100‑1 (MQ=255)                    tataATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCAAATATAAAGTTa                                                                           <  1:3390330/100‑1 (MQ=255)                     ataATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCAAATATAAAGTTaa                                                                          <  1:1771326/100‑1 (MQ=255)                     ataATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCAAATATAAAGTTaa                                                                          <  1:3472262/100‑1 (MQ=255)                      taATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCAAATATAAAGTTAAt                                                                         <  1:62939/100‑1 (MQ=255)                           tGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCAAATATAAAGTTaggaatct                                                                    <  1:496732/100‑8 (MQ=255)                                ataATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCAAATATAAAGTTAATTCTCTTGTTa                                                               <  1:788256/100‑1 (MQ=255)                                    tgtgAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCAAATATAAAGTTAATTCTCTTGTTAATTg                                                           <  1:4481942/100‑1 (MQ=255)                                                            tAACCTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCAAATATAAAGTTAATTCTCTTGTTAATTGTATTTTGAACGATTTCATCATTTg                                   <  1:4612519/100‑1 (MQ=255)                                                               ccTAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCAAATATAAAGTTAATTCTCTTGTTAATTGTATTTTGAACGATTTcatcatgtcacc                                <  1:1840517/100‑7 (MQ=255)                                                                 tAAACGTAGTACTAGTACTGCAAAAATAAATACAATCACACCAAATATAAAGTTAATTCTCTTGTTAATTGTATTTTGAACGATTTCATCATTTGAtttc                              <  1:1036489/100‑2 (MQ=255)                                                                       tagtactagtacTGCAAAAATAAATACAATCACACCAAATATAAAGTTAATTCTCTTGTTAATTGTATTTTGAACGATTTCATCATTTGATTTTTCtttt                        <  1:1084732/100‑1 (MQ=255)                                                                            ctagtactGCAAAAATAAATACAATCACACCAAATATAAAGTTAATTCTCTTGTTAATTGTATTTTGAACGATTTCATCATTTGATTTTTCTTTTAGTCt                   <  1:2726390/100‑1 (MQ=255)                                                                             tagtactGCAAAAATAAATACAATCACACCAAATATAAAGTTAATTCTCTTGTTAATTGTATTTTGAACGATTTCATCATTTGATTTTTCTTTTAGTCtt                  <  1:1293442/100‑1 (MQ=255)                                                                             tagtactGCAAAAATAAATACAATCACACCAAATATAAAGTTAATTCTCTTGTTAATTGTATTTTGAACGATTTCATCATTTGATTTTTCTTTTAGTCtt                  <  1:2221988/100‑1 (MQ=255)                                                                                tactGCAAAAATAAATACAATCACACCAAATATAAAGTTAATTCTCTTGTTAATTGTATTTTGAACGATTTCATCATTTGATTTTTCTTTTAGGCtttgg               <  1:5498186/100‑3 (MQ=255)                                                                                            aaataCAATCACACCAAATATAAAGTTAATTCTCTTGTTAATTGTATTTTGAACGATTTCATCATTTGATTTTTCTTTTAGTCTTTTTAACAAAActacc   <  1:2632487/100‑1 (MQ=255)                                                                                             aataCAATCACACCAAATATAAAGTTAATTCTCTTGTTAATTGTATTTTGAACGATTTCATCATTTGATTTTTCTTTTAGTCTTTTTAACAAAActacct  <  1:5170934/100‑1 (MQ=255)                                                                                             aataCAATCACACCAAATATAAAGTTAATTCTCTTGTTAATTGTATTTTGAACGATTTCATCATTTGATTTTTCTTTTAGTCTTTTTAACAAAActacct  <  1:5072440/100‑1 (MQ=255)                                                                                             aataCAATCACACCAAATATAAAGTTAATTCTCTTGTTAATTGTATTTTGAACGATTTCATCATTTGATTTTTCTTTTAGTCTTTTTAACAAAActacct  <  1:46009/100‑1 (MQ=255)                                                                                             aataCAATCACACCAAATATAAAGTTAATTCTCTTGTTAATTGTATTTTGAACGATTTCATCATTTGATTTTTCTTTTAGTCTTTTTAACAAAActacct  <  1:4406438/100‑1 (MQ=255)                                                                                             aataCAATCACACCAAATATAAAGTTAATTCTCTTGTTAATTGTATTTTGAACGATTTCATCATTTGATTTTTCTTTTAGTCTTTTTAACAAAActacct  <  1:1699641/100‑1 (MQ=255)                                                                                             aataCAATCACACCAAATATAAAGTTAATTCTCTTGTTAATTGTATTTTGAACGATTTCATCATTTGATTTTTCTTTTAGTCTTTTTAACAAAActacct  <  1:596100/100‑1 (MQ=255)                                                                                                 |                                                                                                TAATGTTTTCATCATTTTTTATAATTTGTTTATAATGTGAGCCTTGTGCGATTTGTAAATAACCTAAACGTAGTACTAGTACTGCAAAAATAAATATAATCACACCAAATATAAAGTTAATTCTCTTGTTAATTGTATTTTGAACGATTTCATCATTTGATTTTTCTTTTAGTCTTTTTAACAAAACTACCT  >  NC_007793/1663632‑1663823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑