Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,642,361	C→A	V139V (GTG→GTT)	SAUSA300_RS08115 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,642,361	0	C	A	100.0%	15.0 / NA	8	V139V (GTG→GTT)	SAUSA300_RS08115	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (1/7);  total (1/7)

TTATTGCGCTTCCAGCTCTAAAACGTGAAACACACTCAGCTTTTTCAGCCTTTTTAATTAATAAAGGCACTTTTAAATATGTTTTATAATCATCAATTGCAATATCAATTCTTGTAAAATTTGCATTAACTGATAAACATTTGTCAAAAAAATCTTGCCAAG  >  NC_007793/1642294‑1642455                                                                    |                                                                                               ttATTGCGCTTCCAGCTCTAAAACGTGAAACACACTCAGCTTTTTCAGCCTTTTTAATTAATAAAGGAACTTTTAAATATGTTTTATAATCATCAATTGc                                                                <  1:830996/100‑1 (MQ=255)  tATTGCGCTTCCAGCTCTAAAACGTGAAACACACTCAGCTTTTTCAGCCTTTTTAATTAATAAAGGAACTTTTAAATATGTTTTATAATCATCAATTGCa                                                               <  1:383051/100‑1 (MQ=255)    ttGCGCTTCCAGCTCTAAAACGTGAAACACACTCAGCTTTTTCAGCCTTTTTAATTAATAAAGGAACTTTTAAATATGTTTTATAATCATCAATTGCAat                                                             <  1:4453014/100‑1 (MQ=255)                    aaaaCGTGAAACACACTCAGCTTTTTCAGCCTTTTTAATTAATAAAGGAACTTTTAAATATGTTTTATAATCATCAATTGCAATATCAATTCTTGTaaaa                                             >  1:3190030/1‑100 (MQ=255)                         gTGAAACACACTCAGCTTTTTCAGCCTTTTTAATTAATAAAGGAACTTTTAAATATGTTTTATAATCATCAATTGCAATATCAATTCTTGTAAAATTTGc                                        <  1:5540177/100‑1 (MQ=255)                                acacTCAGCTTTTTCAGCCTTTTTAATTAATAAAGGAACTTTTAAATATGTTTTATAATCATCAATTGCAATATCAATTCTTGTAAAATTTGCATTAACt                                 <  1:5654343/100‑1 (MQ=255)                                                             ataaAGGAACTTTTAAATATGTTTTATAATCATCAATTGCAATATCAATTCTTGTAAAATTTGCATTAACTGATAAACATTTGTCAAAAAAATCTTGCCa    <  1:4744192/100‑1 (MQ=255)                                                               aaaGGAACGTTTAAATATGTTTTATAATCATCAATTGCAATATCAAGGAGGGTAAAATTTGCAGGAAAGGAGAAACATTTGGAAAAAAAAGCTTGCCaaa  <  1:5424087/100‑2 (MQ=255)                                                                    |                                                                                               TTATTGCGCTTCCAGCTCTAAAACGTGAAACACACTCAGCTTTTTCAGCCTTTTTAATTAATAAAGGCACTTTTAAATATGTTTTATAATCATCAATTGCAATATCAATTCTTGTAAAATTTGCATTAACTGATAAACATTTGTCAAAAAAATCTTGCCAAG  >  NC_007793/1642294‑1642455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑