Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,638,046	C→T	E610E (GAG→GAA)	SAUSA300_RS08095 ←	ATPase AAA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,638,046	0	C	T	100.0%	18.4 / NA	9	E610E (GAG→GAA)	SAUSA300_RS08095	ATPase AAA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (1/8);  total (1/8)

TTCGTACCATCACTAAAAACAAGTTTTAAAATATTGTTTTGCGTTTTTTCAAATAATAAATCGCCTGCATTTTTTACATGCTCATCTTCATTATTCTGTAATTTTTCAATTACATTTAATGAACCAACTTTTTTACCATTTTCACGTTCTTCAACCGTATCA  >  NC_007793/1637966‑1638127                                                                                 |                                                                                  tctgTACCATCACTAAAAACAAGTTTTAAAATATTGTTTTGCGTTTTTTCAAATAATAAATCGCCTGCATTTTTTACATGTTCATCTTCATTATTCTGTa                                                                <  1:1482121/97‑1 (MQ=255)                                                          cgaTCTCCTGCATTTTTTACATGTTCATCTTCATTATTCTGTAATTTTTCAATTACATTTAATGAACCAACTTTTTTACCATTTTCACGTTCTTCAACCg       <  1:2754526/98‑1 (MQ=255)                                                           taTCGCCTGCATTTTTTACATGTTCATCTTCATTATTCTGTAATTTTTCAATTACATTTAATGAACCAACTTTTTTACCATTTTCACGTTCTTCAACCGt      <  1:5050765/99‑1 (MQ=255)                                                           atctgCCTGCATTTTTTACATGTTCATCTTCATTATTCTGTAATTTTTCAATTACATTTAATGAACCAACTTTTTTACCATTTTCACGTTCTTCAACCGt      <  1:2681160/96‑1 (MQ=255)                                                              tgCCTGCATTTTTTACATGTTCATCTTCATTATTCTGTAATTTTTCAATTACATTTAATGAACCAACTTTTTTACCATTTTCACGTTCTTCAACCGTATc   <  1:3135403/99‑1 (MQ=255)                                                              tgCCTGCATTTTTTACATGTTCATCTTCATTATTCTGTAATTTTTCAATTACATTTAATAAACCAACTTTTTTACCATTTTCACGTTCTTCAACCGTATc   <  1:3473210/99‑1 (MQ=255)                                                              gatcTGCATTTTTTACATGTTCATCTTCATTATTCTGTAATTTTTCAATTACATTTAATGAACCAACTTTTTTACCATTTTCACGTTCTTCAACCGTATc   <  1:4568298/97‑1 (MQ=255)                                                              cGCCTGCATTTTTTACATGTTCATCTTCATTATTCTGTAATTTTTCAATTACATTTAATGAACCAACTTTTTTACCATTTTCACGTTCTTCAACCGTATc   <  1:4371296/100‑1 (MQ=255)                                                               gCCTGCATTTTTTACATGTTCATCTTCATTATTCTGTAATTTTTCAATTACATTTAATGAACCAACTTTTTTACCATTTTCACGTTCTACAAccagatcg  >  1:3765904/1‑94 (MQ=255)                                                                                 |                                                                                  TTCGTACCATCACTAAAAACAAGTTTTAAAATATTGTTTTGCGTTTTTTCAAATAATAAATCGCCTGCATTTTTTACATGCTCATCTTCATTATTCTGTAATTTTTCAATTACATTTAATGAACCAACTTTTTTACCATTTTCACGTTCTTCAACCGTATCA  >  NC_007793/1637966‑1638127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑