Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,636,070	A→T	I419I (ATT→ATA)	SAUSA300_RS08090 ←	cell division protein FtsK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,636,070	0	A	T	100.0%	47.4 / NA	18	I419I (ATT→ATA)	SAUSA300_RS08090	cell division protein FtsK
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (9/9);  total (9/9)

TTGTTTCACATCTTCCACAAAATCATATTGTTCCATTAAAGGTGCATCAAATAAAATTGGTTCATTATATTGTCCAAGTATAGAAATATAACCTCGGCCGATATCACTATCATGAATCGTTTGAAATTCTTTATCAGTTTGACCAAAGGTCATACGATAACCATCATTTGACAAATTACCT  >  NC_007793/1635986‑1636166                                                                                     |                                                                                                 gctcttccgaTCTTCCACGAAATCATATTGTTCCATTAAAGGTGCATCAAATAAAATTGGTTCATTATATTGTCCAAGTATAGATATGTAACCTCGGCCg                                                                                   <  1:3484619/91‑1 (MQ=255) gctcttccgaTCTTCCACAAAATCATATTGTTCCATTAAAGGTGTATCAAATAAAATTGGTTCATTATATTGTCCAAGTATAGATATATAACCTCGGCCg                                                                                   <  1:1995156/91‑1 (MQ=255)  gtcttccgaTCTTCCACGAAATCATATTGTTCCATTAAAGGTGCATCAAATAAAATTGGTTCATTATATTGTCCAAGTATAGATATATAACCTCGGcctg                                                                                  <  1:5082096/92‑3 (MQ=255)   tcttccgaTCTTCCACAAAATCATATTGTTCCATTAAAGGTGCATCAAATAAAATTGGTTCATTATATTGTCCAAGTATAGATATATAACCTCGGCCGat                                                                                 <  1:428350/93‑1 (MQ=255)              tCCACAAAATCATATTGTTCCATTAAAGGTGCATCAAATAAAATTGGTTCATTATATTGTCCAAGTATAGATATATAACCTCGGCCGATATCACTATCAt                                                                      >  1:480386/1‑100 (MQ=255)                gacaAAATCATATTGTTCCATTAAAGGTGCATCAAATAAAATTGGTTCATTATATTGTCCAAGTATAGATATATAACCTCGGCCGATATCACTATCATGa                                                                    >  1:362230/2‑100 (MQ=255)                cactAAATCATATTGTTCCATTAAAGGTGCATCAAATAAAATTGGTTCATTATATTGTCCAAGTATAGATATATAACCTCGGCCGATATCACTATCATgc                                                                    <  1:3312110/100‑2 (MQ=255)                                       taagTGCATCAAATAAAATTGGTTCATTATATTGTCCAAGTATAGATATATAACCTCGGCCGATATCACTATCATGAATCGTTTGAAATTCTTTATCAGt                                             >  1:920374/4‑100 (MQ=255)                                          gTGCATCAAATAAAATTGGTTCATTATATTGTCCAAGTATAGATATATAACCTCGGCCGATATCACTATCATGAATCGTTTGAAATTCTTTATCAGTTTg                                          <  1:2852900/100‑1 (MQ=255)                                             cATCAAATAAAATTGGTTCATTATATTGTCCAAGTATAGATATATAACCTCGGCCGATATCACTATCATGAATCGTTTGAAATTCTTTATCAGTTTGAcc                                       <  1:2832790/100‑1 (MQ=255)                                                   aTAAAATTGGTTCATTATATTGTCCAAGTATAGATATATAACCTCGGCCGATATCACTATCATGAATCGTTTGAAATTCTTTATCAGTTTGACCAAAGGt                                 <  1:697874/100‑1 (MQ=255)                                                              tCATTGTATTGTCCAAGTATAGATATATAACCTCGGCCGATATCACTATCATGAATCGTTTGAAATTCTTTATCAGTTTGACCAAAGGTCATACGATAAc                      >  1:209174/1‑100 (MQ=255)                                                                          gcAAGTATAGATATATAACCTCGGCCGATATCACTATCATGAATCGTTTGAAATTCTTTATCAGTTTGACCAAAGGTCATACGATAACCATCATTTGACa          >  1:3086381/2‑100 (MQ=255)                                                                          ccAAGTATAGATATATAACCTCGGCCGATATCACTATCATGAATCGTTTGAAATTCTTTATCAGTTTGACCAAAGGTCATACGATAACCATCATTTGACa          >  1:3759734/1‑100 (MQ=255)                                                                          ccAAGTATAGATATATAACCTCGGCCGATATCACTATCATGAATCGTTTGAAATTCTTTATCAGTTTGACCAAAGGTCATACGATAACCATCATTTGACa          >  1:2955843/1‑100 (MQ=255)                                                                           cAAGTATAGATATATAACCTCGGCCGATATCACTATCATGAATCGTTTGAAATTCTTTATCAGTTTGACCAAAGGTCATACGATAACCATCATTTGACaa         >  1:3335194/1‑100 (MQ=255)                                                                                ataGATATATAACCTCGGCCGATATCACTATCCTGAATCGTTTGAAATTCTTTATCAGTTTGACCAAAGGTCATACGATAACCATCATTTGACAAATTAc    >  1:5489849/1‑100 (MQ=255)                                                                                  aGATATATAACCTCGGCCGATATCACTATCATGAATCGTTTGAAATTCTTTATCAGTTTGACCAAAGGTCATACGATAACCATCATTTGACAAATTACCt  <  1:3159742/100‑1 (MQ=255)                                                                                     |                                                                                                 TTGTTTCACATCTTCCACAAAATCATATTGTTCCATTAAAGGTGCATCAAATAAAATTGGTTCATTATATTGTCCAAGTATAGAAATATAACCTCGGCCGATATCACTATCATGAATCGTTTGAAATTCTTTATCAGTTTGACCAAAGGTCATACGATAACCATCATTTGACAAATTACCT  >  NC_007793/1635986‑1636166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑