Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,635,367	A→G	N199N (AAT→AAC)	SAUSA300_RS08085 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,635,367	0	A	G	100.0%	15.7 / NA	8	N199N (AAT→AAC)	SAUSA300_RS08085	membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (2/6);  total (2/6)

AATTCATTACTAAATATGTTCTATATATCGTCATATCAAATAATTGATTACGTATATGATTCATACCTGAATTCTCATGAATATAATCAATATTACCATCAATAGAAGATGTACTATTCATAATTATATTATTTAAGTCCTTACCAATATTGTTTGT  >  NC_007793/1635276‑1635432                                                                                            |                                                                  aaTTCATTACTAAATATGTTCTATATATCGTCATATCAAATAATTGATTACGTATATGATTCATACCTGAATTCTCATGAATATAATCAATGTTCCCATc                                                           <  1:4574183/100‑1 (MQ=255)    tgaccaCTAAATATGTTCTATATATCGTCATATCAAATAATTGATTACGTATATGACTCATACCTGAATTCTCATGAACATAATCAATGTTCCCATCAAt                                                        >  1:4215409/6‑100 (MQ=255)    tCATTACTAAATATGTTCTATATATCGTCATATCAAATAATTGATTACGTATATGATTCATACCTGAATGCTCATGAATATAATCAATGTTCCCATCAAt                                                        <  1:5489755/100‑1 (MQ=255)       ttACTAAATATGTTCTATATATCGTCATATCAAATAATTGATTACGTATATGATTCATACCTGAATTCTCATGAATATAATCAATGTTCCCATCGATaga                                                     <  1:3089215/100‑1 (MQ=255)       ttACTAAATATGTTCTATATATCGTCATATCAAATAATTGATTACGTATATGATTCATACCTGAATTCTCATGAATATAATCAATGTTCCCATCAATaga                                                     <  1:4485110/100‑1 (MQ=255)                                              gATTACGTATATGATTCATACCTGAATTCTCATGAATATAATCAATGTTCCCATTAATAGAAGATGTACTATTCATAATTATATTATTCAAATCCTTAcc              <  1:4855518/100‑1 (MQ=255)                                                    gTATATGATTCATACCTGAATTCTCATGAATATAATCAATGTTCCCATCAATAGAAGATGTACTATTCATAATTATATTATTCAAATCCTTACCAATAtt        <  1:3717989/100‑1 (MQ=255)                                                          gATTCATACCTGAATTCTCATGAATATAATCAATGTTCCCATCAATAGAAGATGTACTATTCATAATTATATTATTCAAATCCTTACCAATAttgtttgt  >  1:2611153/1‑100 (MQ=255)                                                                                            |                                                                  AATTCATTACTAAATATGTTCTATATATCGTCATATCAAATAATTGATTACGTATATGATTCATACCTGAATTCTCATGAATATAATCAATATTACCATCAATAGAAGATGTACTATTCATAATTATATTATTTAAGTCCTTACCAATATTGTTTGT  >  NC_007793/1635276‑1635432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑