Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,634,467	G→A	N499N (AAC→AAT)	SAUSA300_RS08085 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,634,467	0	G	A	100.0%	51.8 / NA	20	N499N (AAC→AAT)	SAUSA300_RS08085	membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/16);  total (4/16)

CGTACTTGATGTTCTTGCTGTGATGCTTTTTGTGGTTGTTTAGAATAATTATTTGCATTTGATTCAACAGAAGCAGATTTTACATCGTTACCATTCATTGACTCTTGTTGTTCTCTGCGCTTATTACCATTTTTTTGATTATCTTTATTTATAAATAAAGGCATTGGTTTATCCTGTTGTCT  >  NC_007793/1634381‑1634562                                                                                       |                                                                                                cGTACTTGATGCTCTTGCTGTGATGCTTTTTGTGGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTCACATCATTACCATTCATTg                                                                                    <  1:5601980/100‑1 (MQ=255)         aTGCTCTTGCTGTGATGCTTTTTGTGGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCttgg                                                                            <  1:1288535/100‑2 (MQ=255)           gctCTTGCTGTGATGCTTTTTGTGGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCttgttc                                                                          <  1:4391742/98‑2 (MQ=255)            ctCTTGCTGTGATGCTTTTTGTGGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCTtgttgt                                                                         <  1:2298706/99‑1 (MQ=255)            ctCTTGCTGTGATGCTTTTTGTGGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCTtgttgt                                                                         <  1:2555726/99‑1 (MQ=255)                tGCTGTGATGCTTTTTGTGGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCTTGTTGTtctt                                                                     <  1:2707439/100‑2 (MQ=255)                tGCTGTGATGCTTTTTGTGGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCTTGTTGTtctc                                                                     >  1:2100382/1‑100 (MQ=255)                   cgtgATGCTTTTTGTGGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCTTGTTGTTctcttc                                                                  >  1:4123479/2‑98 (MQ=255)                    gtgATGCTTTTTGTGGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCTTGTTGTTCTCTtcg                                                                 >  1:963361/1‑100 (MQ=255)                       aTGCTTTTTGTGGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCTTGTTGTTCTCTTCGCtt                                                              <  1:5342878/100‑1 (MQ=255)                        tGCTTTTTGTGGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCTTGTTGTTCTCTTCGCtag                                                             <  1:731035/100‑3 (MQ=255)                          cTTTTTGTGGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCTTGTTGTTCTCTTCGCttatt                                                           <  1:2150312/100‑1 (MQ=255)                          cTTTTTGTGGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCTTGTTGTTCTCTTCGCttatt                                                           <  1:1205545/100‑1 (MQ=255)                             tttGTGGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCTTGTTGTTCTCTTCGCTTATTAcc                                                        <  1:1561637/100‑1 (MQ=255)                               tgtgGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCTTGTTGTTCTCTTCGCTTATTACCAt                                                      <  1:5052398/100‑1 (MQ=255)                                         tAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCTTGTTGTTCTCTTCGCTTATTACCATTTTTTTGAtt                                            <  1:86374/100‑1 (MQ=255)                                          aGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCTTGTTGTTCTCTTCGCTTATTACCAttttttgcacac                                           <  1:5443803/100‑7 (MQ=255)                                                                 cAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCTTGTTGTTCTCTTCGCTTATTACCATTTTTTTGATTATCTTTATTTATAAATAAAGGCAt                    >  1:4708625/1‑100 (MQ=255)                                                                      gAAGCAGATTTTACATCATTACCATTCATTGACTCTTGTTGTTCTCTTCGCTTATTACCATTTTTTTGATTATCTTTATTTATAAATAAAGGCATTGGtc               <  1:4390476/100‑2 (MQ=255)                                                                                  aCATCATTACCATTCATTGACTCTTGTTGTTCTCTTCGCTTATTACCATTTTTTTGATTATCTTTATTTATAAATAAAGGCATTGGTTTATCCtgctgta   <  1:1140850/100‑2 (MQ=255)                                                                                   catcatTACCATTCATTGACTCTTGTTGTTCTCTTCGCTTATTACCATTTTTTTGATTATCTTTATTTATAAATAAAGGCATTGGTTTATCCTGCTGtca  <  1:1788472/100‑2 (MQ=255)                                                                                       |                                                                                                CGTACTTGATGTTCTTGCTGTGATGCTTTTTGTGGTTGTTTAGAATAATTATTTGCATTTGATTCAACAGAAGCAGATTTTACATCGTTACCATTCATTGACTCTTGTTGTTCTCTGCGCTTATTACCATTTTTTTGATTATCTTTATTTATAAATAAAGGCATTGGTTTATCCTGTTGTCT  >  NC_007793/1634381‑1634562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑