Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007793	1,634,430	T→C	N512D (AAT→GAT)	SAUSA300_RS08085 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007793	1,634,430	0	T	C	100.0%	48.2 / NA	18	N512D (AAT→GAT)	SAUSA300_RS08085	membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (3/15);  total (3/15)

CCACTTGTGGCGACCTTTGTATATCTTTTCGCTGTCTTGTTTCTCGTACTTGATGTTCTTGCTGTGATGCTTTTTGTGGTTGTTTAGAATAATTATTTGCATTTGATTCAACAGAAGCAGATTTTACATCGTTACCATTCATTGACTCTTGTTGTTCTCTGCGCTTATTACCATTTTTTTGATTA  >  NC_007793/1634337‑1634521                                                                                              |                                                                                            ccACTTGTGGCGACCTTTGTATATCTTTTCGCTGTCTTGTTTTTCGTACTTGATGCTCTTGCTGTGATGCTTTTTGTGGTTGTTTAGAATAATCATTTGc                                                                                       <  1:3854490/100‑1 (MQ=255)                                             cGTACTTGATGCTCTTGCTGTGATGCTTTTTGTGGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTCACATCATTACCATTCATTg                                           <  1:5601980/100‑1 (MQ=255)                                                     aTGCTCTTGCTGTGATGCTTTTTGTGGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCttgg                                   <  1:1288535/100‑2 (MQ=255)                                                       gctCTTGCTGTGATGCTTTTTGTGGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCttgttc                                 <  1:4391742/98‑2 (MQ=255)                                                        ctCTTGCTGTGATGCTTTTTGTGGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCTtgttgt                                <  1:2298706/99‑1 (MQ=255)                                                        ctCTTGCTGTGATGCTTTTTGTGGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCTtgttgt                                <  1:2555726/99‑1 (MQ=255)                                                            tGCTGTGATGCTTTTTGTGGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCTTGTTGTtctt                            <  1:2707439/100‑2 (MQ=255)                                                            tGCTGTGATGCTTTTTGTGGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCTTGTTGTtctc                            >  1:2100382/1‑100 (MQ=255)                                                               cgtgATGCTTTTTGTGGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCTTGTTGTTctcttc                         >  1:4123479/2‑98 (MQ=255)                                                                gtgATGCTTTTTGTGGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCTTGTTGTTCTCTtcg                        >  1:963361/1‑100 (MQ=255)                                                                   aTGCTTTTTGTGGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCTTGTTGTTCTCTTCGCtt                     <  1:5342878/100‑1 (MQ=255)                                                                    tGCTTTTTGTGGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCTTGTTGTTCTCTTCGCtag                    <  1:731035/100‑3 (MQ=255)                                                                      cTTTTTGTGGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCTTGTTGTTCTCTTCGCttatt                  <  1:1205545/100‑1 (MQ=255)                                                                      cTTTTTGTGGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCTTGTTGTTCTCTTCGCttatt                  <  1:2150312/100‑1 (MQ=255)                                                                         tttGTGGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCTTGTTGTTCTCTTCGCTTATTAcc               <  1:1561637/100‑1 (MQ=255)                                                                           tgtgGTTGTTTAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCTTGTTGTTCTCTTCGCTTATTACCAt             <  1:5052398/100‑1 (MQ=255)                                                                                     tAGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCTTGTTGTTCTCTTCGCTTATTACCATTTTTTTGAtt   <  1:86374/100‑1 (MQ=255)                                                                                      aGAATAATCATTTGCATTTGATTCAACAGAAGCAGATTTTACATCATTACCATTCATTGACTCTTGTTGTTCTCTTCGCTTATTACCAttttttgcacac  <  1:5443803/100‑7 (MQ=255)                                                                                              |                                                                                            CCACTTGTGGCGACCTTTGTATATCTTTTCGCTGTCTTGTTTCTCGTACTTGATGTTCTTGCTGTGATGCTTTTTGTGGTTGTTTAGAATAATTATTTGCATTTGATTCAACAGAAGCAGATTTTACATCGTTACCATTCATTGACTCTTGTTGTTCTCTGCGCTTATTACCATTTTTTTGATTA  >  NC_007793/1634337‑1634521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑